Biomedical subjects

D Ioannides

Publications and source records attributed to D Ioannides.

21 records · Page 2Linked to original sources

PubMed1993

Association of tissue-fixed cytoplasmic deposits of immunoglobulin in epidermal keratinocytes with lupus erythematosus.

BACKGROUND AND DESIGN: We have observed that tissue-fixed deposits of immunoglobulin (Ig) are occasionally present in the cytoplasm (CYT) of epidermal keratinocytes. This study was conducted to examine the frequency, significance, and possible cause of this finding. RESULTS: Direct immunofluorescence studies were performed on 302 sequential skin specimens obtained from patients with lupus erythematosus (n = 98), bullous diseases (n = 114), or other dermatoses (n = 90). Abnormal epidermal CYT deposits were observed in 22 of the patients with lupus erythematosus (22.5%), four of those with bullous diseases (3.5%), and two of those with other dermatoses (2.2%). The deposits were more common in systemic than in discoid lupus erythematosus, and in skin lesions than in normal skin. By indirect immunofluorescence, circulating antibodies to epidermal upper CYT antigens were present in 100% of 12 patients with tissue-fixed CYT deposits (average antibody titer, 300) and in 21% of 19 individuals without CYT deposits (average titer, 43). By Western immunoblot analysis these antibodies were directed to proteins in keratin extracts of skin. CONCLUSIONS: These findings indicate that deposits of Ig in the cytoplasm of keratinocytes in human skin are associated with high levels of serum antibodies to upper CYT antigens and with lupus erythematosus.

Autoantibodies↗

PubMed1992

Immunofluorescence abnormalities in lichen planopilaris.

BACKGROUND: Lichen planopilaris is believed to be a variant of lichen planus because both diseases have similar histologic features. However, as the clinical features of the two diseases differ, we conducted immunofluorescence studies to examine the relation between the two conditions more closely. OBSERVATIONS: Direct immunofluorescence was performed on scalp lesions of seven patients with lichen planopilaris. All had abnormal linear deposits of Ig restricted to the basement membrane zone of hair follicles. The deposits consisted of only IgG or IgA in five patients (70%) and of IgG in combination with other Igs in two patients (30%). Basement membrane zone deposits of fibrin were present in only one patient (14%) and were linear in appearance. There was no staining of ovoid bodies. These immunofluorescence abnormalities differ from those associated with lichen planus where basement membrane zone deposits of fibrin are present in almost all patients, where the basement membrane zone deposits are fibrillar in appearance, and where the deposits of Ig over ovoid bodies are common. CONCLUSIONS: The different appearance and composition of abnormal deposits of immunoreactants in lichen planopilaris and lichen planus suggest that the two conditions are different diseases.

Adult↗

PubMed1991

Regional variation in the expression of pemphigus foliaceus, pemphigus erythematosus, and pemphigus vulgaris antigens in human skin.

The expression of the pemphigus foliaceus (PF), pemphigus erythematosus (PE), and pemphigus vulgaris (PV) antigens in 16 different regions of normal human skin was evaluated by indirect immunofluorescence by using sera with a high titer of PF, PE, and PV antibodies. Regional variations were observed in the expression of all these antigens. The expression of the PF and PE antigens, as measured by endpoint titer of antibody reactivity, was highest in skin specimens obtained from the upper torso, and lowest in those from the buccal mucosa, lower torso, and scalp. This distribution pattern differed from that of PV antigen, whose expression was highest in buccal mucosa and scalp. These patterns correlate with, and may provide a partial explanation for, the different distribution of skin lesions in these different forms of pemphigus.

Antigens, Surface↗