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D Ionescu

Publications and source records attributed to D Ionescu.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

Mental stress enhances the sympathetic fraction of QT variability in an RR-independent way.

In 10 healthy male volunteers aged 19-20, spectral power of the beat-by-beat QT interval was measured at the Traube-Herring-Mayer (THM) band (0.05 to 0.15 Hz) when the subject was at rest and during atrial pacing. After resting in dorsal decubitus for 10 minutes, right atrial pacing was performed at a slight elevation above sinus rhythm as well as at 100 or at 110 beats per minute for 7 minutes each. In addition, during pacing at 100 or 110 bpm, the subject was required to perform a Kraepelin Arithmetic test. There was a statistically significant increase in QT spectral power at the THM band, while heart rate was maintained unchanged during the periods of mental stress versus rest. This experiment confirms the potential independence of the QT interval from heart rate and suggests that THM fraction of QT spectral power has a double subordination: during relaxed rest it follows mostly RR-fluctuation; during stress an RR-independent contribution is added, which presumably reflects the supra-normal sympathetic drive on the ventricles.

Adult↗
PubMedDate not supplied

[Fertility in prolactinomas--considerations concerning some clinical cases].

Prolactinoma is a frequent endocrine cause of infertility in both man and women. The aim of this study was to evaluate female fertility in association with hyperprolactinemia. The study is a retrospective one, based on the cases monitored in the Endocrinology Department of Iaşi. From the 113 cases of prolactinoma in women we have selected 83 women aged between 18-45 years (fertile age). The diagnosis was suspected on clinical bases: endocrine (amenorrhea-galactorrhea, associated or isolated, spaniomenorrhea, infertility), and tumoral (headache, visual disturbances) syndromes and confirmed by biological (PRL dosage) and morphological (pituitary CT) tests. We classified the found associations between prolactinoma and fertility in: no correlation (previous pregnancies with no desire for another child--76 patients = 75.2%); prolactinoma diagnosed immediately after a pregnancy by persistency of galactorrhea, lack of menses reapparition, headache (16 cases = 15.8%); prolactinoma diagnosed before pregnancy, which was obtained thanks to the hyperprolactinemia treatment (9 patients = 8.9%). All 9 patients of the last group were in remission at the moment of pregnancy apparition and had a normal pregnancy, giving birth to normal children. Infertility associated with prolactinoma is reversible with treatment. Lowering of prolactin levels to normal is often necessary to permit ovulation. Bromocriptine used by pregnant women appears to be safe for the developing fetus, at least in our study where the treatment was interrupted in most cases in the first trimester of pregnancy.

Adolescent↗
PubMedDate not supplied

[Hyperprolactinemia and pregnancy].

Prolactin is a polipeptidic hormone secreted by the anterior pituitary gland, whose main role consists in the stimulation of lactation in the postpartum period. The increasing of prolactin secretion can be physiological (pregnancy and lactation) or pathological (hypothalamic and pituitary diseases, iatrogenic, etc.). The suppression of the hypothalamic-pituitary-gonadal axis and the resistivity of the ovary and gonadotropin action are induced in hyperprolactinemia, the amenorrhea and lack of ovulation being the result. Infertility associated with hyperprolactinemia is reversible with treatment, irrespectively to the type of treatment (radical or medical). Lowering of prolactin levels to normal or near normal is often necessary to permit ovulation. Dopamine-like drogs, as bromocriptine, appear to be safe for the developing fetus, at lest when the treatment is interrupted in the first trimester of pregnancy.

Bromocriptine↗
PubMedDate not supplied

[A possible protocol for postoperative management in laparoscopic cholecystectomy].

The goal of the "Prospect" programme (sponsored by Pfizer) is to create possible evidence-based protocols related to the management of postoperative pain after certain type of surgical intervention (e.g. hernia repairs, hysterectomies, etc.). This article is introducing the protocol for laparoscopic cholecystectomy for both day-case and longer hospital admission cases. The protocol is designed for preoperative, intra and postoperative period, choosing only those measures which were effective for postoperative pain, published in the literature. We are also presenting an analyze of our 13,000 laparoscopic cholecystectomies, from "Prospect" protocol point of view, and what we should do to improve the management of postoperative pain.

Analgesics↗
PubMedDate not supplied

[New bacterial microorganisms in the etiology of human infections. The genus Gardnerella].

Haemophilus vaginalis first affiliated in the bacterial systems in the genus Haemophilus, the Corynebacterium due to its morphophysiological characteristics--forms a new genus Gardnerella with only one species G. vaginalis. The bacterium is important for man's pathology, as it is responsible for the most numerous vaginitis, considered nonspecific. The paper reports synthetically the present knowledge in biology, pathogenicity, diagnosis and treatment.

Animals↗
PubMedDate not supplied

[Non-culturable Koch bacilli during antitubercular chemotherapy].

The authors have performed 58435 microscopic examinations, of which 3813 were positive, and found 209 (5,48%) cases of non-cultivable Koch bacilli. The major bacteriological, clinico-evolutive and therapeutical aspects are examined. Most of the cases showing a syndrome of positive homogenate with a negative culture were rather recent ones (an evolution of less than 2 years in 73,68% of the total) and the highest frequency was found in those in which the treatment was in its first year. No direct relationship could be demonstrated between the number of germs (the Gaffky scale) and the frequency of the syndrome. The factors influencing the occurrence and the development of the syndrome are: the structure of the lesions, the method of bacteriologic examination, the therapy applied, and a certain immunologic reactivity. According to the authors the significance of this syndrome is positive in most of the cases, indicating a regression that has not been completed. The most reasonable attitude is a differentiated one, depending on each case, integrating the data obtained by the bacteriological examination in the therapeutic and evolutive clinico-radiologic context.

Antitubercular Agents↗
PubMedDate not supplied

Separation of a glycoprotein antigen for the diagnosis of bovine leukemia and some of its biochemical properties.

Data are presented on the separation and on some biochemical properties of an antigen to be used in the immunodiffusion (ID) test for detection of bovine leukemia virus. The antigen obtained proved to have the same efficiency in the ID test as a commercially available standard antigen. Experimental results suggest that the antigen is released into the culture fluid in the form of a glycoprotein molecule consisting of two subunits with a molecular weight of 60,000 linked by--SH bonds.

Animals↗
PubMedDate not supplied

Isolation of anti-carcinoembryonic antigen (CEA), specific immunoglobulins and their use in immunocytochemical and enzyme-immunoassays (ELISA).

Anti-carcinoembryonic (CEA) polyclonal antibodies in sheep and rabbits were raised using purified CEA from acid extracts of human colon adenocarcinoma. CEA was purified by gel filtration on Sepharose 4B CL and chromatography on DEAE-Sephadex A50. The antiserum was adsorbed with human serum and perchloric acid extract from normal colon. Anti-CEA IgG was purified from monospecific antiserum by ion-exchange chromatography and its specificity was tested on cryostat sections from colon adenocarcinoma by the indirect immunoperoxidase technique. The specific reaction was compared with that obtained by using a similar technique and two CEA specific monoclonal antibodies. An anti-CEA IgG peroxidase conjugate was obtained allowing to establish a "sandwich" ELISA-CEA system with two antibodies. CEA determinations were made in a group of 15 normal controls (mean value 4.8 +/- 0.4 ng/ml) and in 30 colorectal tumor patients (mean value 26.6 +/- 2.15 ng/ml). The anti-CEA antibodies are proven useful in immunocytochemical and ELISA techniques and may be further used in radioimaging of tumors.

Adenocarcinoma↗
PubMedDate not supplied

[The isolation of anti-carcinoembryonic antigen (CEA) immunoglobulins and their use in immunocytochemical and immunoenzyme (ELISA) technics].

Carcinoembryonic anti-antigen (CEA) polyclonal antibodies were obtained in ram and rabbit using as antigen source the extracts with perchloric acid from the human colon adenocarcinomas. CEA was purified by gel filtration on Sepharose 4BCL and ion-exchange chromatography (DEAE-Sephadex A50). The total antiserum was absorbed with human serum and perchloric acid extract from the normal colon. IgG anti-CEA was purified by chromatography of the monospecific antiserum, then tested for bonding specificity, at cryostat on sections of colon adenocarcinoma, by indirect immunoperoxidase. The specific reaction was compared with that obtained by the same technique, using two monoclonal antibodies specific to the CEA molecule (MAb-26/3/13 and MAb-26/5/1 respectively). IgG anti-CEA was also used for obtaining some IgG-peroxidase conjugates, with an immunoenzymatic system, ELISA type, with two antibodies according to the model of the ELISA kits produced by the Cantacuzino Institute (ELISA-AFP). The ELISA-CEA kit was standardized using an international CEA standard. CEA was quantitatively determined with this immunoenzymatic system (ELISA-CEA) on a group of 15 healthy subjects (average: 4.8 +/- 0.12 ng/ml) and on 30 patients with colorectal tumours (average: 26.6 +/- 0.15 ng/ml). ELISA-CEA kit, sensitive and reproducible, allow the usual quantitative determination of CEA, a useful marker in diagnosing and monitoring tumour evolution.

Antibody Specificity↗