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Biomedical subjects

D J Bush

Publications and source records attributed to D J Bush.

13 recordsLinked to original sources
PubMed2006

Effect of testing temperature on internal egg quality measurements.

The objective of this study was to determine the effect of egg testing temperature on quality measurements of shell eggs. The quality measurements compared included 3 Haugh unit (HU) devices (electronic Haugh, tripod Haugh, and Haugh meter), egg weight, albumen height, albumen width, albumen index, yolk width, yolk height, yolk index, percentage of thin albumen, and vitelline membrane strength at 3 temperatures of 5, 13, and 23 degrees C from 2 strains of laying hens (Hyline W36 and Bovans White) at 2 storage times. The HU measurements averaged 72.44 at time zero and 59.99 at 7 wk. At 7 wk for all devices, HU values decreased 6 units with increased temperature (P < 0.05). The electronic Haugh and tripod Haugh devices gave equal measurements for all testing conditions. The Haugh meter gave equal values at 5 degrees C for fresh eggs but lower HU at higher temperatures and 7 wk storage. Thus, it is recommended that egg testing temperature be reported when HU are measured. Coefficient of variation generally increased for all HU methods with increasing temperature. Although there was a proportionately different amount of thin albumen detected between the strains of laying hens, no significant difference was seen in HU. From the evaluated methods for measuring quality, the electronic Haugh, which electronically measures albumen height and calculates HU, provided the lowest coefficient of variation, was sensitive to quality loss, and gave the highest quality measurement (5 degrees C).

Albumins↗
PubMed2001

A comparison of 1% prilocaine with 0.5% ropivacaine for outpatient-based surgery under axillary brachial plexus block.

UNLABELLED: We compared the use of 1% prilocaine with 0.5% ropivacaine for axillary brachial plexus anesthesia in a double-blinded manner in day-stay patients to determine the better of the two local anesthetics in terms of onset time and duration of motor block. Sixty patients scheduled for outpatient upper-limb surgery were allocated randomly to receive either prilocaine (28 patients) or ropivacaine (32 patients) at a volume of 0.7 mL/kg. The brachial plexus was located with a plexus needle and nerve stimulator. By 20 min after injection of prilocaine or ropivacaine, there was no difference in analgesic effect. By this time, it was apparent whether or not a block was going to be adequate for surgery. Pain returned after a mean of 278 min (SD 111 min; range, 160-630 min) with prilocaine as compared with 636 min (SD 284 min; range, 210-1440 min) with ropivacaine. Analgesia use was similar in both groups. Duration of motor block with prilocaine was a mean of 254 min (SD 62 min; range, 130-385 min), as compared with 642 min (SD 199 min; range, 350-1080 min) with ropivacaine. We conclude that there is no clinically important difference between 1% prilocaine and 0.5% ropivacaine in time to onset of axillary brachial plexus block when they are injected in equal volumes. There is a significantly longer duration of action with ropivacaine, which may make it less suitable for day-stay upper-limb surgery because of the handicap from reduced muscle power. IMPLICATIONS: This study compares two local anesthetics to determine which is most suitable for day-stay upper-limb surgery under axillary brachial plexus block. Prilocaine 1% is more suitable than ropivacaine 0.5% because of a more prolonged duration of action of ropivacaine, although this could be useful in other circumstances.

Adult↗
PubMed1995

A comparison of informed consent for obstetric anaesthesia in the USA and the UK.

The practice of 75 UK and 75 US obstetric anaesthetists in obtaining informed consent for obstetric anaesthesia (for caesarean section) and obstetric analgesia (for labour) was compared using a postal questionnaire. The response rate was approximately 60% for each group. Of the US anaesthetists 47% obtained separate written consent for obstetric anaesthesia compared to 22% of the UK group (P=0.012). Corresponding percentages for epidural analgesia were 52% for the US, and 15% for the UK (P < 0.001). Significantly more of the listed risks and benefits relating to general anaesthesia were discussed by the US anaesthetists compared to the UK group, median (interquartile range), 6 (4-7) and 3 (1-4), P < 0.001. There was no significant difference in discussion before regional anaesthesia but the US group discussed more information before epidural analgesia for labouring mothers obtunded by pain or drugs. These results suggest that discussion and documentation of informed consent for obstetric anaesthesia and analgesia could be improved in both countries, especially the UK.

Journal Article↗
PubMed1994

Cervical carcinoma in women less than 35 years of age.

We retrospectively studied 80 patients, less than 35 years old, who were being treated for invasive cervical carcinoma at Charity Hospital of Louisiana in New Orleans. The period covered by the study was March 1980 to November 1989. The study group represented 9.3% (80/862) of the total patients seen with the disease. Disease stage was IB in 50 patients, IIA in 4, IIB in 14, IIIA in 5, IIIB in 6, and IVA in 1 patient. Histopathologic classes included 74 squamous cell carcinomas, 3 adenosquamous carcinomas, 2 adenocarcinomas, and 1 anaplastic carcinoma. Treatment used was either radical hysterectomy, irradiation alone, or irradiation followed by hysterectomy. Five-year actuarial survival rates were as follows: stage IB, 81.6%; stage IIA, 25.0%; stage IIB, 29.8%; stage IIIA, 20.0%; and stage IIIB, 33.4%. The only patient with stage IV cancer died of disease. Our findings do not reveal a relationship between age and survival in stage IB carcinoma of the cervix, and the numbers in the other stages are too small to comment on.

Adult↗
PubMed1994

Feline serum antibody responses to Toxoplasma gondii and characterization of target antigens.

The Toxoplasma gondii-specific target antigens for feline immunoglobulin M (IgM) and immunoglobulin G (IgG) immune responses were studied longitudinally using western blot immunoassay in 8 cats experimentally inoculated with T. gondii strain ME49. Multiple antigens were recognized by IgM and IgG during the course of infection. Dense bands were associated with 12 antigens in the IgM western blot immunoassay and 30 antigens in the IgG western blot immunoassay. Immunoglobulin M responses were maximal on week 4 postinoculation (PI) and were greatly diminished by week 20 PI. Immunoglobulin G responses were maximal on week 12 PI. On week 20 PI, the 19-kDa (6/8 samples), 26-kDa (8/8 samples), 28-kDa (8/8 samples), 31-kDa (7/8 samples), 35-kDa (6/8 samples), 51-kDa (6/8 samples), 55-kDa (7/8 samples), and 65-kDa (7/8 samples) antigens were recognized most commonly in the IgG western blot immunoassay. When the western blot immunoassay results were compared to enzyme-linked immunosorbent assay (ELISA) results, there was no clear advantage to the development of IgM-ELISA, IgG-ELISA, IgM western blot immunoassay, or IgG western blot immunoassay using a single antigen instead of multiple antigens as the detection system for the diagnosis of recent infection.

Animals↗
PubMed1992

Diclofenac for analgesia after caesarean section.

The analgesic efficacy of a single intramuscular dose of 75 mg diclofenac given after elective Caesarean section was studied in 50 women in a double-blind randomised manner using a patient-controlled analgesia system. The mean 18 h papavaretum consumption of the placebo group was significantly greater (91.4 mg compared to 61.4 mg). Subjective experience of pain and observed sedation were significantly greater in the control group up to 6 h after operation.

Adult↗
PubMed1976

Appraising current therapy for breast cancer. 2. Irradiation.

The best combination of methods for managing primary breast cancer may not yet be known. Radiation therapy has been proved effective in decreasing the incidence of local and regional recurrence following mastectomy. Thus, until a superior regimen is established, I recommend local and regional irradiation for patients at high risk for recurrence. Local irradiation is also an effective means of achieving palliation of symptoms associated with metastatic disease.

Breast Neoplasms↗
PubMed1973

Microtubules: evidence for 13 protofilaments.

When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed: those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented.

Animals↗