Rapid protein matrix reduction by a hydrophobic interaction-based solid phase extraction method.
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Animals↗
Biomedical subjects
D J Gisch
Publications and source records attributed to D J Gisch.
Shielded hydrophobic phase: a new concept for direct injection analysis of biological fluids by high-performance liquid chromatography.
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Carbamazepine↗
Determination of monosaccharides in glycoproteins by reversed-phase high-performance liquid chromatography on 2.1-mm narrowbore columns.
The monosaccharide composition of glycoproteins was determined by using a Supelcosil LC-18 150 x 2.1 mm narrowbore column under reversed-phase conditions. Methodology was developed for sample hydrolysis, N-acetylation, benzoylation, and cleanup prior to high-performance liquid chromatography. Monosaccharides were identified and quantified at picomole levels. Various glycoproteins were then analyzed, including ribonucleases A and B, Mucin, and fetuin. In addition, the monosaccharide content of Chinese hamster ovary cell-expressed recombinant human renin was determined.
Chemical Phenomena↗
Pretreatment of urine samples for the analysis of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid using solid-phase extraction.
An extraction and concentration procedure using a unique solid-phase extraction tube was developed for the analysis of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid in urine. Absolute and relative recoveries of the drug metabolite were found to be greater than 85% and 92%, respectively. The extraction procedure allows quantitation of the drug metabolite by GC/MS, HPLC with UV detection, or GC with flame ionization detection. The extraction procedure was verified with urine samples obtained from marijuana users as well as spiked urine samples.
Chromatography, Gas↗