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Biomedical subjects

D J Lee

Publications and source records attributed to D J Lee.

At least 19 recordsLinked to original sources
PubMed2005

Steady-state humic-acid-containing blanket in upflow suspended bed.

We investigated the effects of turbidity and concentration of humic acid on the steady-state behavior of the blanket, which was coagulated using polyaluminum chloride (PACl) as coagulant. The three-dimensional solid-flux plot was constructed. Based on fixed PACl dosage, the iso-humic-acid solid-flux surfaces stacked that enveloped the feasible regime for the blanket bed. The steady-state point moved toward low solid flux and low solid fraction regime with decreasing initial raw water turbidity and/or increasing humic-acid concentration. Low water turbidity and high humic-acid concentration yielded a bulky blanket, with the former producing clean, and the latter turbid effluent. The presence of humic acid was thereby harmful to blanket strength, except for the case of low raw water turbidity. An optimal range of humic acid for blanket strength and clarification efficiency existed at 1 mg l(-1). Low level of humic acid is beneficial to blanket development with low-turbidity raw water.

Aluminum Chloride↗
PubMed2005

Comparison of dewaterability and floc structure of synthetic sludge with activated sludge.

This work compared the appearance, dewaterability, floc size, fractal dimensions derived from free-settling test and light-scattering test, and detailed structural information of floc interior including porosity, Sierpinski carpet fractal dimension, and the fractal dimension of the pore boundaries of a synthetic sludge and an activated sludge. The present synthetic sludge could probe the change in filterability and in fractal dimensions extracted from small angle light scattering test and from free-settling test after conditioning, however, failed to reproduce the detailed structural information of sludge flocs. The use of specific synthetic sludge depends on the field of interest. For instance, the present synthetic sludge is not appropriate to simulate the intrafloc transport of solute owing to its incapability to reproduce the detailed floc structure or to its change after conditioning.

Flocculation↗
PubMed2005

Statistical mechanics of columnar DNA assemblies.

Many physical systems can be mapped onto solved or "solvable" models of magnetism. In this work, we have mapped the statistical mechanics of columnar phases of ideally helical rigid DNA--subject to the earlier found unusual, frustrated pair potential (A.A. Kornyshev, S. Leikin, J. Chem. Phys. 107, 3656 (1997))--onto an exotic, unknown variant of the XY model on a fixed or restructurable lattice. Here, the role of the "spin" is played by the azimuthal orientation of the molecules. We have solved this model using a Hartree-Fock approximation, ground-state calculations, and finite-temperature Monte Carlo simulations. We have found peculiar spin order transitions, which may also be accompanied by positional restructuring, from hexagonal to rhombohedric lattices. Some of these have been experimentally observed in dense columnar aggregates. Note that DNA columnar phases are of great interest in biophysical research, not only because they are a useful in vitro tool for the study of DNA condensation, but also since these structures have been detected in living matter. Within the approximations made, our study provides insight into the statistical mechanics of these systems.

Computer Simulation↗
PubMed2004

Force of a gas bubble on a foreign particle in front of a freezing interface.

We monitored the formation and development of a single gas bubble on the surface of a spherical particle of size 1.676 mm under unidirectional freezing and thawing (4.6-5.0 microm/s) and for the first time quantitatively estimated the force exerted on this particle by measuring the deformation of an attached elastic stick. The bubble would nucleate and grow on the particle surface closest to the ice front, while the force curve for a freezing-thawing cycle presented a hysteresis characteristic. This force was much greater than in the case without a bubble, and hence it dominated the engulfment process in the present freezing tests. The bubble force increased with increasing bubble size and was shown to be mainly attributable to the elastic force by the deformed bubble shape. Comments were made on the need to incorporate the role of bubbles in predicting the critical velocity to freeze a suspension with high dissolved gas content.

Journal Article↗
PubMed2004

DNA-DNA interaction beyond the ground state.

The electrostatic interaction potential between DNA duplexes in solution is a basis for the statistical mechanics of columnar DNA assemblies. It may also play an important role in recombination of homologous genes. We develop a theory of this interaction that includes thermal torsional fluctuations of DNA using field-theoretical methods and Monte Carlo simulations. The theory extends and rationalizes the earlier suggested variational approach which was developed in the context of a ground state theory of interaction of nonhomologous duplexes. It shows that the heuristic variational theory is equivalent to the Hartree self-consistent field approximation. By comparison of the Hartree approximation with an exact solution based on the QM analogy of path integrals, as well as Monte Carlo simulations, we show that this easily analytically-tractable approximation works very well in most cases. Thermal fluctuations do not remove the ability of DNA molecules to attract each other at favorable azimuthal conformations, neither do they wash out the possibility of electrostatic "snap-shot" recognition of homologous sequences, considered earlier on the basis of ground state calculations. At short distances DNA molecules undergo a "torsional alignment transition," which is first order for nonhomologous DNA and weaker order for homologous sequences.

Base Pairing↗
PubMed2004

Advective flow in a sludge floc.

The interior of sludge floc is highly heterogeneous, while the large pores in the floc control the advective flow. This work for the first time numerically details fluid flow and mass transfer processes in pores of activated sludge floc. The dimensionless permeabilities and mass dispersion coefficients were contoured against pore size ratio and the floc Reynolds number. With a pore size less than 20% of the floc size, the commonly adopted homogeneous model overestimates the floc permeability, and pore velocity is less than 2% of the bulk velocity. This is particularly true for flocs with low porosity. Although the convective flux is low, the dispersive mass transfer rate can be much higher than the diffusional rate, attributable to the strong Taylor dispersion effect. The three-dimensional pore structures in waste activated-sludge floc were identified using confocal laser scanning microscope (CLSM) images. Large pores were used to numerically estimate the permeability and dispersion coefficient for these pores. The permeability and the dispersion coefficient of the tortuous pores can be one order of magnitude lower than those for the equivalent straight pores. Besides the dispersion effect, the pore tortuosity appeared as the most important geometrical factor retarding the advective flow in the sludge pores. In addition, the small side pores connected to the large pore had only a mild effect on the flow process, and can be neglected in analysis.

Journal Article↗
PubMed2004

Bilevel thresholding of floc images.

Frequently encountered problems on bilevel thresholding of floc images include information loss during the image conversion stage, sampling bias owing to spatially inhomogeneous luminous flux over an optical microscope image or among a series of images, thresholding error adopting various algorithms, and resampling errors during three-dimensional reconstruction. Among these steps the choice of thresholding algorithm could yield most of the processing error. Ways to reduce the potential processing errors are discussed.

Journal Article↗
PubMed2004

SEM/EDS evaluation of porcelain adherence to gold-coated cast titanium.

The adhesion between titanium and dental porcelain is related to the diffusion of oxygen to the reaction layer formed on cast-titanium surfaces during porcelain firing. The diffusion of oxygen could be suppressed by coating the titanium surface with a thin gold layer. This study characterized the effects of gold coating on titanium-ceramic adhesion. ASTM grade II CP titanium was cast into a MgO-based investment (Selevest CB, Selec). The specimen surfaces were air abraded with 110-microm Al(2)O(3) particles. Gold coating was applied on titanium surfaces by three methods: gold-paste (Deck Gold NF, Degussa-Ney) coating and firing at 800 degrees C for three times, single gold-paste coating and firing followed by sputter coating (40 mA, 500 s), and sputter coating (40 mA, 1000 s). Surfaces only air abraded with Al(2)O(3) particles were used as controls. An ultra-low-fusing dental porcelain (Vita Titankeramik, Vident) was fused on titanium surfaces. Specimen surfaces were characterized by SEM/EDS and XRD. The titanium-ceramic adhesion was evaluated by a biaxial flexure test (N = 8), and area fraction of adherent porcelain (AFAP) was determined by EDS. Numerical results were statistically analyzed by one-way ANOVA and the Student-Newman-Keuls test at alpha = 0.05. SEM fractography showed a substantial amount of porcelains remaining on the gold-sputter-coated titanium surfaces. A new Au(2)Ti phase was found on gold-coated titanium surface after the firing. Significantly higher (p <.05) AFAP values were determined for the gold-sputter-coated specimens compared to the others. No significant differences were found among the other groups and the control. Results suggested that gold coatings used in this study are not effective barriers to completely protect titanium from oxidation during the porcelain firing, and porcelain adherence to cast titanium can be improved by gold-sputter coating used in the present study.

Dental Porcelain↗
PubMed2004

Bilevel thresholding of sliced image of sludge floc.

This work examined the feasibility of employing various thresholding algorithms to determining the optimal bilevel thresholding value for estimating the geometric parameters of sludge flocs from the microtome sliced images and from the confocal laser scanning microscope images. Morphological information extracted from images depends on the bilevel thresholding value. According to the evaluation on the luminescence-inverted images and fractal curves (quadric Koch curve and Sierpinski carpet), Otsu's method yields more stable performance than other histogram-based algorithms and is chosen to obtain the porosity. The maximum convex perimeter method, however, can probe the shapes and spatial distribution of the pores among the biomass granules in real sludge flocs. A combined algorithm is recommended for probing the sludge floc structure.

Algorithms↗
PubMed2004

Effect of pre-hydrolysis on floc structure.

Morphological changes in flocs were elucidated by small-angle light scattering, free settling tests and microscopic observation to determine the effects of pre-hydrolysis (alkaline treatment and ultrasonication) on two sludges--primary raw sludge from the Malabar Sewage Treatment Plant, Sydney (sludge M) and biological sludge collected at the nitrogen removal unit of St Marys Sewage Treatment Plant in Sydney (sludge S). Ultrasonication or alkaline treatment released a marked amount of insoluble organic matter in a soluble form. The latter treatment was more efficient than the former. Meanwhile, the pre-hydrolyzed flocs had more compact structures than the original ones, as shown by their higher free-settling fractal dimension, lower areal porosity and smaller internal pores. In particular, alkaline treatment yielded flocs with more compact interiors than did ultrasonication. Such detailed structural information could not be elicited by monitoring the change in floc size. The greater resistance to mass transfer of the hydrolyzed flocs thus produced does not reduce the efficiency of subsequent digestion, indicating that the surface reaction rate on the solid surface might have dominated since the pre-hydrolysis steps modified the local chemical environment to promote digestion.

Bioreactors↗
PubMed2004

Blanket dynamics in upflow suspended bed.

A one-dimensional model for the spatio-temporal variation of solids fraction in the blanket considering both the hydrodynamic dispersion and the convection effects was proposed in this study. The model equation was a wave equation whose solution presented shock-wave like characteristic. The model output predicted that the distribution of the solids fraction in the blanket would, firstly, evolve to a uniform distribution at a lower solids fraction. Afterward, the blanket would compact itself to another uniform distribution at a higher solids fraction. Experimental works with synthetic raw water coagulated with PACl suspended in a lab-scale suspended bed confirmed the theoretical predictions. The solids flux plot was constructed with underdosed, optimal and overdosed suspensions. Change in coagulant dose would affect the blanket height and the response time. The solids flux curve was shifted accordingly. The "optimal' operational condition for the upflow suspended bed that produced the lowest effluent turbidity was identified and discussed.

Flocculation↗
PubMed2004

Structure of conditioned sludge flocs.

Free settling tests, small-angle light scattering, microtome-slicing techniques, and confocal laser scanning microscopy were performed to examine how the cationic flocculation or freezing and thawing affected the floc structure. The floc size, internal pore size, mass fractal dimensions determined from free-settling test or small angle light scattering test, aeral porosity, boundary fractal dimension and Sierpinski carpet fractal dimension of pore boundary from 2D slices, and the volume porosity, compactness, and the pores' box-counting fractal dimension from 3D reconstructed image, were estimated and compared. Cationic flocculation would produce large flocs with internal pores of shape resembling a long "tube" with rough surface. Freezing and thawing would produce flocs with internal pores with lower aspect ratio and a smoother boundary.

Bacteria↗
PubMed2004

Effects of rancidity and free fatty acids in choice white grease on growth performance and nutrient digestibility in weanling pigs.

Two experiments were conducted to determine the effects of rancidity and FFA in choice white grease (CWG) on growth performance and nutrient digestibility in nursery pigs. In Exp. 1,150 crossbred pigs (average initial BW of 6.8 kg and average initial age of 21 d) were used. Treatments (as-fed basis) were a corn-soybean meal-based control with no added fat, 6% CWG, and 6% CWG heated at 80 degrees C, with oxygen gas bubbled through it at 849 mL/min for 5, 7, 9, or 11 d. Peroxide value for the CWG increased as oxidative exposure was increased from 0 to 7 d (i.e., peroxide values of 1, 40, and 105 mEq/kg for d 0, 5, and 7, respectively), but decreased to 1 mEq/kg as the hydroperoxides decomposed after 9 and 11 d of oxidation. Pigs fed the control diet (no added fat) had the same (P = 0.91) overall ADG (d 0 to 35) but lower G:F (P < 0.04) than pigs fed diets with added fat. As for the effects of fat quality, ADG (linear effect, P < 0.01) and ADFI (linear effect, P < 0.001) decreased as the fat was made more rancid. However, there were no changes in digestibility of fatty acids as the rancidity of the fat was increased (P = 0.16), suggesting that the negative effects of rancidity were from decreased food intake and not decreased nutrient utilization. In Exp. 2, 125 crossbred pigs (average initial BW of 6.2 kg and average initial age of 21 d) were used to determine the effects of FFA in CWG on the growth performance and nutrient digestibility in nursery pigs. Treatments (as-fed basis) were a corn-soybean meal-based control with no added fat, 6% CWG, and 6% CWG that had been treated with 872, 1,752 or 2,248 lipase units/g of fat. The FFA concentrations in the CWG were increased from 2% with no lipase added to 18, 35, and 53% as lipase additions were increased. Pigs fed the control diet (no added fat) had the same (P = 0.30) overall ADG (d 0 to 33) but lower G:F (P < 0.01) than pigs fed diets with added fat. There were no effects of FFA concentration on ADG (P = 0.18), and ADFI increased (linear effect, P < 0.04) as FFA concentration in the CWG increased. Fatty acid digestibility was not affected (P = 0.17) by FFA in the diet. In conclusion, our data suggest that as fat is oxidized (especially to peroxide values greater than 40 mEq/kg), ADG and ADFI in nursery pigs will decrease; however, FFA concentrations of at least 53% do not adversely affect utilization of CWG in nursery pigs.

Animal Feed↗
PubMed2004

A comparison of ultrasound treatment on primary and secondary sludges.

Ultrasound treatment of primary and secondary sludges was conducted to improve the qualities of sludges for the anaerobic digestion. The impacts of different sonication times, sonication densities and solids concentrations on ultrasonication efficiency were examined. The experimental results indicated that the significant reduction in particle size and increase in soluble organics could be achieved, implying that ultrasonication could offer a feasible treatment method to efficiently disintegrate sludge. The greater decrease in particle size and increase in soluble organics of sludge indicated that the secondary sludge has a more remarkable improvement after sonication over the primary sludge. With respects to the extent of disintegration and energy consumption, higher sonication density performed more effectively in terms of specific energy. There exists an optimal solids concentration range for both the sludges for optimum sonication. Within the optimal solids concentration range, efficient sonication can be effected and sludge would be disintegrated efficiently. The ultrasound would be attenuated by scattering and absorption if the solids concentration exceeds the optimal range. It appeared from the study that the mechanical shear forces caused by ultrasonic cavitation could be a key factor for sludge disintegration and collapse of cavitation bubbles could significantly alter the sludge characteristics.

Particle Size↗
PubMed2004

Thermal drying of wastewater sludge with crack formation.

We examined in this work the drying characteristics of wastewater sludge. The drying flux of the constant-rate period for sludge cake could be up to 40% higher than that from a sand bed. Owing to the considerable volume shrinkage of cake, cracks would form and develop on the crack surface, which yielded three-dimensional but rather than the one-dimensional cake structure assumed in conventional drying theories. The crack length was fully developed in the first 30 min of drying, while the width of crack increased linearly with time. Using these data the drying flux from the cracks was estimated. Enhanced drying flux was noticeable which depended on the crack shape and the crossflow velocity.

China↗
PubMed2004

Reuse of wastewater sludge with marine clay as a new resource of construction aggregates.

The disposal of sludge from wastewater treatment presents highly complex problems to any municipality. Most of the sludge disposal methods have varying degrees of environmental impact. Hence, it is necessary to explore potential areas of reuse in order to alleviate sludge disposal problems and to conserve natural resources. Industrial sludge and marine clay are two forms of high-volume wastes. Using these wastes as a resource of raw materials to produce construction aggregates would enable large-scale sludge reuse. The aggregates were produced at various sludge-clay combinations containing 0, 20, 50, 80 and 100% clay contents, respectively. The pelletized aggregates displayed lower particle densities ranged between 1.48 and 2.25 g/cm3, compared to the density of granite at 2.56 g/cm3. Good 28-day concrete compressive strength of 38.5 N/mm2 achieved by the 100% sludge aggregate was comparable to the value of 38.0 N/mm2 achieved of the granite control specimens. The leachate contamination levels from the aggregates after 150 days were found acceptable when used in concrete, indicating insignificant environmental contamination. The heat flow study showed increases in heat flow at the temperatures of 480 degrees C and between 660 degrees C and 900 degrees C, indicating a need for the extension of heating time around these temperatures.

Aluminum Silicates↗
PubMed2004

Production of hydrogen and methane from wastewater sludge using anaerobic fermentation.

The hydrogen and methane were produced from wastewater sludge using a Clostridium strain. The original sludge and the pre-treated (acidified, sterilized, freeze/thawed, and sonicated) sludges were tested. Some pre-treatment could enhance hydrogen yield, and the other tests could enhance methane yield. Hydrogen yield followed freeze/thawed>acidified>sterilized>original sludge>sonicated; while methane yield followed sonicated>freeze/thawed>sterilized>acidified>original sludge. The production and consumption of acetate correlated closely with the trends in both yields.

Acetic Acid↗