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Biomedical subjects

D J Nalebuff

Publications and source records attributed to D J Nalebuff.

7 recordsLinked to original sources
PubMed1992

In vitro testing methodologies. Evolution and current status.

Almost 25 years after the development of the radioallergosorbent test, in vitro technology has become firmly established as an outstanding diagnostic tool for physicians treating the allergic patient. The American Academy of Otolaryngic Allergy (AAO), in its most current position statement, endorses the modified RAST as reproducible, sensitive, and specific. A new organization called the American In Vitro Allergy and Immunology Society (AIVAIS) has been formed to promote and monitor the use of these tests. Both societies suggest that in vitro tests should be part of a complete clinical evaluation by a well informed physician knowledgeable about the contraindications and limitations of in vitro technology. Such physicians should be capable of incorporating this information into responsible, judicious, and effective management of allergic patients. Only after the correct diagnosis has been made can appropriate treatment be started. Whichever route you choose, you and your patients can benefit from the use of in vitro allergy testing.

Allergens↗
PubMed1979

Localization of IgE in adenoids and tonsils: an immunoperoxidase study.

An immunoperoxidase technique was applied to the localization of IgE in formaldehyde-solution-fixed, paraffin-embedded tissue. The content and distribution of IgE in tonsil and adenoid tissues from ten patients with histories of inhalant allergies and elevated serum levels of total and allergen-specific IgE were investigated. Compared with tissues from five nonatopic subjects, the tissues from atopic individuals were observed to harbor a much larger population of IgE-formating plasma cells. The profusion of IgE plasma cells in nasopharyngeal lymphoid tissue, observed in this study, furnishes strong anatomic evidence for a pathogenetic role of IgE in inhalant allergies. For cellular and tissue localization of IgE, the immunoperoxidase technique offers clear advantages over previously used immunoflorescence procedures.

Adenoids↗
PubMed1979

Localization of IgE in tissues by an immunoperoxidase technique.

An indirect immunoperoxidase technique was used to study the content and distribution of IgE in formaldehyde solution-fixed, paraffin-embedded adenoid and nasal tissues of atopic and nonatopic persons. Adenoid tissue from 15 patients, and nasal tissue from five patients with symptoms of inhalant allergies and augmented serum levels of total and allergen-specific IgE were examined with this method. Adenoid tissues from five patients without allergic symptoms were studied for comparison. A rich content of IgE, largely within the cytoplasm of the plasma cells, was observed in tissues of atopic subjects. The sections from nonallergic persons contained few weakly staining IgE-positive cells. These observations provide anatomic support for the role of IgE in hypersensitivity reaction of immediate type. This immunoperoxidase technique, circumventing the principal shortcomings of the immunofluorescence procedures, affords a highly sensitive and practical approach to cellular and tissue localization of IgE.

Adenoids↗
PubMed1979

An immunoperoxidase assay for serum ragweed-specific IgE.

A solid-phase enzyme immunoassay for allergen-specific IgE antibodies in serum is descirbed. In this technique these antibodies are allowed to bind to the allergen previously adsorbed to the wells of polystyrene microtiter plates. After a washing step the tubes are incubated with rabbit antihuman IgE labelled with horseradish peroxidase. Following a second washing step, the enzyme bound to the tubes is assayed spectrophotometrically using O-phenylene diamine as the substrate. The standard curves obtained with this method and with the RAST technique are illustrated. For the assay of serum antiragweed IgE antibodies, concordance between the results obtained with the RAST test and this immunoperoxidase assay was observed in 85 or 95 (90%) patients with symptoms of ragweed hayfever. The coefficients of variation ranged from 4.4% (2SD +/- .010) TO 14% (2SD +/- .008). The advantages of using peroxidase as the enzymatic marker for the assay of allergen-specific IgE are discussed.

Allergens↗
PubMedDate not supplied

An immunoperoxidase method for the demonstration of allergen-specific IgE in serum.

A solid-phase immunoenzymatic technique for the detection of allergen-specific IgE antibodies in serum is described. The binding of such antibodies to allergen insolubilized on cyanogen bromide-activated paper discs was detected by a subsequent two-step procedure involving the use of rabbit antihuman IgE and goat antirabbit IgG coupled with peroxidase. A solution of 3-3' diaminobenzidine and hydrogen peroxide, employed as the color indicator system, turned the discs dark brown in positive cases. Discs carrying 11 different inhalant allergens were tested with sera containing allergen-specific IgE antibodies. Agreement between the results of this technique and the RAST was seen in 85% of 310 tests performed. In the clinical practice of otorhinolaryngologic allergy, this test may prove to be an important laboratory adjunct ot clinical history and diagnostic skin test in the identification of the incriminated inhalant allergens.

Allergens↗