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Biomedical subjects

D J Stechschulte

Publications and source records attributed to D J Stechschulte.

62 records · Page 4Linked to original sources
PubMed1967

Antibodies involved in antigen-induced release of slow reacting substance of anaphylaxis (SRS-A) in the guinea pig and rat.

The antigen-induced release of SRS-A and histamine was studied in the guinea pig and rat using whole and fractionated antiserum preparations. Guinea pig 7Sgamma(1)-antibody sensitized sliced guinea pig lung tissue for antigen-induced release of both SRS-A and histamine; neither substance was released from lung tissue prepared with 7Sgamma(1)-antibody. Rats injected intraperitoneally with hyperimmune rabbit or rat antiserum released only SRS-A in significant amounts when challenged with antigen by the same route. A definite time interval between the injection of antiserum and challenge with antigen was required for optimal release of SRS-A. Fractionation of rat antiserum demonstrated that the immunoglobulin responsible for most of the SRS-A release from rat peritoneal tissue was a gammaG-antibody or fraction thereof. Acting in this capacity, the gammaG-antibody or its subfraction may be considered a second type of homocytotropic antibody. Fractions of rat antisera containing the first type of homocytotropic antibody, i.e. antibody mediating release of histamine and serotonin, prepared peritoneal tissues for the release of large amounts of these pharmacological agents and only small amounts of SRS-A. Two different mechanisms for the production of PCA lesions in the rat were considered. One of these involves the antigen-induced release of histamine and serotonin from mast cells sensitized by homocytotropic antibody. This reaction has an optimal latent period of 24-72 hr. The second mechanism involves the local combination of antigen with "hyperimmune" heterologous or homologous antisera. This reaction can be elicited after a latent period of 4 but not 24 hr; host complement and leukocyte lysosomal enzymes, as well as SRS-A, may be involved.

Anaphylaxis↗
PubMedDate not supplied

Potential pitfall of the EndoButton.

A clinical and cadaveric example show the EndoButton (Acufex Microsurgical Inc, Mansfield, MA), used for anterior cruciate ligament endoscopic fixation, flipping outside the extensor mechanism or vastus lateralis rather than flipping directly outside the lateral femoral cortex. This pitfall was caused by overdrilling the femoral socket beyond the recommended 6 mm and overadvancing the EndoButton beyond the required depth to flip the EndoButton. Overdrilling the femoral socket to a depth of 10 mm still allows the EndoButton to rest properly on the cortex without soft tissue interposition. Increasing angles of knee flexion at the time of Endobutton placement decrease the safe distance beyond the lateral femoral cortex for flipping without soft tissue interposition. There is also potential to flip the EndoButton within the substance of the vastus lateralis, but the flipping action is blunted and not discrete.

Anterior Cruciate Ligament↗