Biomedical subjects

D Jakubowska

Publications and source records attributed to D Jakubowska.

16 recordsLinked to original sources

PubMed1992

Seasonal variations of antipyrine pharmacokinetics parameters in calves.

In the experiment the values of parameters of antipyrine kinetics were defined (Vd - volume of distribution, t0.5 - half-life, CA - metabolic clearance) in calves in January, April, July and October 1986 and in January and July 1987. Statistically significant increase of distribution volume and increase of hepatic antipyrine clearance were recorded, as well as significant shortening of half-life of this substance in the organism of tested animals in summer 1986 and 1987, compared with the remaining seasons of the year. Antipyrine pharmacokinetics in winter, spring, autumn 1986 and winter 1986/87 did not differ significantly.

Animals↗

PubMed1992

Pharmacokinetics of antipyrine in calves during first 35 days of life.

The experiment was carried out on 10 bull-calves of black-white breed, aged 2-35 days. The following parameters of antipyrine pharmacokinetics were determined: Vd - volume of distribution, t0.5 - biological half-life, CA - metabolic clearance. It has been shown statistically that the volume of antipyrine distribution (expressed in 1/kg) was significantly decreasing with age of examined animals. The antipyrine half-life proved to be the longest on the 20th day of calves' life and the shortest on the 10th and 30th day. The maximum values of metabolic clearance of antipyrine were observed in calves aged 10 days and the minimum ones in animals aged 20 days. In the cases of t0.5 and CA a tendency to changes of values of these parameters of antipyrine pharmacokinetics in 10 days periods was observed.

Animals↗

PubMed1992

Monthly variations in the pharmacokinetics of antipyrine in calves.

In the experiment (on the basis of values of antipyrine (phenazone) pharmacokinetics parameter), biotransformation activity of calves liver during a year was determined. The experiment was carried out on calves aged 28-30 days. Volume of distribution (Vd), half-life (t0.5) and metabolic clearance (CA) of antipyrine were from month to month determined. Not significant changes between values of Vd, t0.5 and CA in several months were observed. Results of experiment indicated that calves' liver is characterized by the relatively stabile biotransformational activity during the whole year.

Animals↗

PubMed1991

[The influence of ACTH and hydrocortisone on drug kinetics in calves in the example of the antipyrine test].

The aim of the experiment carried out on 2 groups (to comprise 8 specimens) bull calves, of ncb. breed, at the age of 60 to 65 days and of an average body weight 90 +/- 5 kg, was to define the effect of intramuscular ACTH (0.2 j.m./kg b.w.) and hydrocortisone (0.2 mg/kg b.w.) injections on the hepatic biotransformation of antipyrine. No changes were noticed in the quantity of antipyrine distribution space Vd under the influence of the hormonal preparations applied. Shorter time of antipyrine halflife from the body T/2 was observed, yet the differences stated have not confirmed statistically. ACTH and hydrocortisone injections caused the increase in constant elimination value of antipyrine from the organism k as well as the increase in the quantity of hepatic antipyrine clearance ClA. The differences observed have not turned out to be statistically significant.

Adrenocorticotropic Hormone↗

PubMed1991

[Twenty-four hour changes in the value of the liver clearance of antipyrine in calves during the neonatal period].

Twenty-four-hour changes in the value of the liver clearance coefficient of antipyrine in 10 calves of ncb. race, aged 28-30 days were examined. The findings made it possible to separate 2 subgroups among the examined animals: 6 calves eliminated antipyrine from plasma more quickly during the day than at night and 4 calves - more quickly at night than in the day. To verify the observed differences 3 days after the first examination another antipyrine test was performed on 6 calves (3 from the "night" group and 3 from the "day" group). It has been found that animals which had the tendency to quicker elimination (metabolism) of the antipyrine during the day confirmed it in the second examination. The same regularity has been observed for the calves which quicker eliminate antipyrine from plasma at night.

Animals↗

PubMed1991

[An attempt at assessment of biotransformation activity in calves' liver on the basis of the rate of antipyrine elimination from saliva].

In the test carried out on 10 calves-bulls of c.b. breed, aged 20-23 days, the usefulness of the rate of antipyrine elimination from saliva to the assessment of pharmacokinetics of this substance, and consequently to the assessment of the rate of liver biotransformation in these animals, was defined. It was stated that volume of antipyrine (phenazone) distribution, half-life period and the clearance of liver defined on the basis of determination of the changes of phenazone concentration in plasma and saliva are not significantly different. It was proved that in the neonatal period in calves the antipyrine fraction connected with plasma protein does not exceed 3 percent of general phenazone concentration in the plasma. On the grounds of the results of the antipyrine test carried out a second time on 5 calves, the frequent recurrence of the parameters of antipyrine kinetics in the tested animals was proved. The results of the experimental show that on the basis of determination of the phenazone concentration in saliva it is possible to define the biotransforming liver activity of the microsomal monoxygenase connected with cytochrome P-450 in calves and total water content in the organism.

Animals↗

PubMed1979

[Health-resort therapy and its importance for geriatric prophylaxis (author's transl)].

The authors report on the influence of a single or repeated health-resort therapy in geriatric sanatoria on the subjective condition, the state of health, and the biochemical parameters.

Aged↗

PubMed1978

Aspartate carbamoyltransferase activity in rat liver at different stages of its development.

Aspartate carbamoyltransferase (ACTase) activity has been measured in rat livers at different stages of their development and in intact and regenerating livers. The level of enzyme activity was found to be the highest in the earliest fetal livers (about 7 times higher than in the adult rat liver). Deproteinized by heating, supernatants of sheep liver homogenates were purified on a Sephadex G-25 column. The influence of deproteinized supernatant on ACTase activity in rat liver was measured. This influence depended on the amount of activating factor added to fresh homogenates. A different scope of activation of enzyme activity in rat liver caused by deproteinized supernatant was noticed. The greatest increase of enzyme activity under the influence of activating factor was obtained in the liver of young animals, mothers and old female rats, the smallest in fetuses and very young animals. A relatively smaller increase of enzyme activity was observed in regenerating livers under the same experimental conditions. In this article the role of natural ACTase activator is discussed.

Aging↗

PubMed1977