Hospital maintenance.
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Hospital Design and Construction↗
Biomedical subjects
D Jay
Publications and source records attributed to D Jay.
An improved microsurgical clamp and stay suture retractor for use in vascular surgery in rats.
Microsurgical procedures require the use of a variety of expensive precision instruments. This expense can be a problem for surgeons working in surgical research laboratories. To help combat rising expenses in our laboratory, we developed a new microsurgical vascular clamp and a new stay suture retractor for use in experimental surgery in rats. These instruments are inexpensive, can be manufactured in any medical machine shop, and have proved to be efficient and effective in surgical procedures.
Animals↗
Superoxide dismutase activity of the salicylate-iron complex.
BACKGROUND: Scavenging of superoxide radical by salicylate-iron complex was studied to determine whether or not the salicylate-iron complex was able to catalyze the dismutation of superoxide radicals, the result perhaps yielding an explanation of the antioxidant and anti-inflammatory properties of the drug. METHODS: The scavenging was studied with an assay that generates O2.- without the intervention of metal ions. RESULTS: Results indicated that, in the presence of iron, salicylate was able to bring about the catalytic dismutation of the superoxide radical. The rate of superoxide removal was dependent on both the concentration of iron and the salicylate:iron molar ratio. CONCLUSIONS: These results may help to explain the interaction of nonsteroidal anti-inflammatory drugs with free radicals and the anti-inflammatory properties of these agents, inasmuch as accumulating evidence indicates that much of the injury observed during inflammatory disorders may be mediated by oxidative stress frequently induced by iron-dependent reactions.
Aerobiosis↗
Captopril and glutathione inhibit the superoxide dismutase activity of Hg (II).
The purpose of this study was to determine if captopril, an angiotensin-converting enzyme inhibitor, and glutathione could interact with mercuric ions and so modify the catalytic dismutation of superoxide carried out by this metal. With an assay that generates superoxide anion radicals without the intervention of metal ions increasing concentrations of both reagents progressively inhibited the breakdown of superoxide brought about by mercury. Maximum inhibition was attained with a molar ratio of captopril (glutathione): Hg (II) = 1. These results may help to explain the protective and/or antioxidative effect of thiol compounds during mercury intoxication.
Angiotensin-Converting Enzyme Inhibitors↗
[Cardiology review. Molecular and genetic aspects of cardiopathies].
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Animals↗
[An in vitro study of the mechanism by which captopril attenuates myocardial reperfusion damage].
The purpose of this study was to establish the molecular mechanism whereby captopril prevents the formation of active oxygen species, since many studies have provided evidence that postischemic myocardial dysfunction is mediated, at least in part, by the generation of these agents. Results indicate that captopril is a potent inhibitor of certain reactions mediated by O2-. However, this interference of captopril was not understood as an scavenging activity against the free radicals generated. The data could be explained by considering a direct interaction of captopril with the metal ions present that catalyse the formation of O2-. This mechanism could be of biological relevance.
Animals↗
Electron paramagnetic resonance of the mitochondrial respiratory chain in the presence of phenazine methosulfate.
The EPR spectra of phenazine methosulfate (PMS) generated under different conditions, as reduction or excitation with light were studied. In addition, results show the EPR spectra of reduced-submitochondrial particles and reduced-submitochondrial particles in the presence of PMS. Combined systems of this last type have been repeatedly utilized in EPR studies. This work proves that such systems give rise to characteristic signals around g = 2, which behaviour reflects the presence of particular prosthetic groups of the respiratory chain combined with the reduced dye. The consequences of these findings are discussed.
Animals↗
[Molecular bases of arterial hypertension induced by cadmium].
In order to establish at subcellular level the biochemical role of cadmium in the etiology of arterial hypertension, we studied the effect of this metal on some mitochondrial functions. The results showed that cadmium inhibits calcium uptake as well as stimulates calcium release in kidney mitochondria. By the same token Cd2+ inhibits the ADP and ATP exchange-reaction and also inhibits succinate oxidation. From these results it is proposed that C d2+ interacts with--SH groups that are important for energy--linked reactions and calcium transport; the above produces metabolic modifications that may result in hypertensive disease.
Adenosine Diphosphate↗