D Kipiotis | PubMed Health

PubMed1990

Canine capillary formation in vitro.

Microvascular endothelial cells derived from canine subcutaneous adipose tissue formed knob-like and tube-like structures in vitro without tumor-conditioned medium or special substrate. The knob-like structures consisted of acidic and basic glycosaminoglycans arranged in order. Knob-like structures were built from cell extrudates and were responsible for capillary lumen formation in vitro. Transmission electron microscopy confirmed the endothelial nature of the cells which expressed extensive phagolysosomal activity.

Animals↗

PubMed1989

Early human trophoblast cell cultures. A morphological and immunocytochemical study.

Rapidly growing cytotrophoblasts were isolated from early human chorionic villi and the Papanicolaou method was used to characterize their cytology and transformation into syncytiotrophoblasts. Cytotrophoblasts fused and formed binucleated cells or mononucleated intermediate cells. Syncytial cells were formed by fusion of small cytotrophoblasts or intermediate cells and cytotrophoblasts. Glycosaminoglycans were produced in cytotrophoblasts and released extracellularly. Here they were accumulated and/or diffused into a continuous layer covering the cells. Glycosaminoglycans in syncytial cells were contained in well defined membranous sacs. Cytotrophoblasts only grown beyond confluence differentiated into villi with a villus-like histology.

Cells, Cultured↗