Biomedical subjects
Publications and source records attributed to D Kisiel.
To obtain the apomucin as a substrate for glycosyltransferases activity testing, the pig gastric mucin deglycosylation by chemical method was carried out. Resulted apomucin, rich in Ser, Thr and Pro residues, was as good carbohydrate acceptor in enzymatic O-glycosylation in vitro, as synthetic peptide, analogue of MUC2 mucin tandem repeats sequence.
PURPOSE: Sugars and polyols content assessment in cataractous nucleus in diabetics and non-diabetics. MATERIAL AND METHODS: Cataractous nucleus was obtained during extracapsular cataract extraction derived from 52 diabetic and 58 non-diabetic patients. The content of glucose, fructose, sorbitol and mioinositol in examined material was determined using iquid gas chromatography. RESULTS: Average contents of sorbitol, glucose and fructose in cataractous nucleus diabetic patients (ZSC) are significantly higher than in non-diabetics (ZS). No changes were observed in mioinositol content in both studied groups. CONCLUSION: The role of the polyol pathway in the development of diabetic cataract is considerable.
O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.
This paper reports our clinical experiences with the electrical high frequency audiometer (EHFA)-Tonndorf, used in conjunction with conventional audiologic site of lesion testing. The EHFA is routinely used in the site of lesion hearing evaluation battery. We are reporting on the first 100 subjects, aged 6-85 years, seen in otologic/neurotologic consultation for complaint(s) of hearing loss, tinnitus, vertigo, ear blockage alone and/or in combination from 1983 to 1985. The significance of the data obtained suggests that: (1) Patient responses with EHFA are influenced by age, degree of hearing loss, degree of ear disease, site of lesion and disease, noise exposure and ototoxicity. (2) The degree of hearing loss as well as symmetry (asymmetry) of the loss affect the EHFA responses. (3) Responses to EHFA can be correlated with subject group classification. Five groups were well defined based upon degree and symmetry (asymmetry) of the hearing loss. (4) Electrical high-frequency audiograms (EHFAs) can be classified into five different patterns of response. (5) EHFA should be part of a site-of-lesion audiometry test battery.