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D Klimek

Publications and source records attributed to D Klimek.

20 records · Page 2Linked to original sources

[Effect of anticoagulants on lymphocyte isolation by density gradient centrifugation].

The authors compared the efficiency of isolation and the purity of lymphocytes obtained by the method of centrifugation in a gradient of ficoll and uropoline from blood obtained on ACD fluid and on heparin. Isolation of lymphocytes from the blood obtained on heparin was more efficient (60%) and useful. For removal of platelets it was necessary to use additional centrifugation in a saccharose gradient or application of aggregating agents during isolation of lymphocytes obtained on heparin or ACD fluid.

Anticoagulants↗