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Biomedical subjects

D Klisenbauer

Publications and source records attributed to D Klisenbauer.

9 recordsLinked to original sources
PubMed2003

UV-B induced production of MMP-2 and MMP-9 in human corneal cells.

The purpose of this study was to determine the production of metalloproteinases (MMP) 2 and 9 following UV-B irradiation in human corneal epithelial cells and fibroblasts. Epithelial cells and fibroblasts were separated from human donor corneas and exposed to UV-B lamp irradiation for 20, 40, 80 and 120 s. Media samples were collected at 8, 24, 48 and 72 h and gelatinase A and B production was assayed by the ELISA test. Statistical significance of production was assessed by the paired t-test. Increased production of MMP-2 was found in human corneal fibroblasts in response to UV-B irradiation. A statistically significant production of MMP-2 was not observed in human corneal epithelial cells following UV-B exposure. We did not detect any increase in MMP-9 after irradiation in either epithelial cells or fibroblasts. MMP-2 is produced by the corneal fibroblasts in the acute phase after UV-B irradiation. MMP-9 is not released in vitro following UV-B irradiation damage and therefore does not directly participate in the pathophysiology of acute photokeratitis.

Cells, Cultured↗
PubMed2000

[The excimer laser and severe myopia--comparison of LASIK and PRK].

The aim of this study is to compare the results of photorefractive keratectomy (PRK) with method of laser-assisted keratomileusis (LASIK) in the treatment of high myopia by excimer laser. The study compares 100 eyes after PRK operated on by technique of multizonal ablation divided into group of moderately high myopia (-6.0 ... -9.0 Dsph/85 eyes) and severely high myopia (over -9.25 Dsph/15 eyes) with a group o 100 eyes operated on by LASIK similarly classified (moderately high myopia/67 eyes and severely high myopia/33 eyes). The eyes were treated by excimer laser Keracor 117 manufactured by Technolas company. Statistic analysis used one-way analysis of variance. One year after operation emmetropia within +/- 1.0 Dsph was 52% in the first and 47% in the second group after PRK. After LASIK it was 71% and 54% respectively. No statistically significant differences have been found between the groups. In spite of this, analysis of postoperative complications, line loss of the preoperative best spectacle corrected acuity and patients satistaction shows that LASIK is a better method in correction of high myopia by excimer laser.

Adult↗
PubMed1994

[Neodymium-YAG laser capsulotomy].

The results of 79 Nd: YAG capsulotomies in patients after extracapsular cataracta extraction are presented. The improvement in visual acuity of one or more Snellen lines in 94.1% eyes was observed. The complications included transitory hypertension more than 40 mmHg in 1.4% eyes, damage to the intraocular lens in 40.3% eyes, disruption of the anterior hyaloid face with forward displacement of vitreous into capsulotomy din 38.8% eyes. The others complications like retinal detachment, corneal damage, hemorrhage or macular edema were not observed.

Cataract Extraction↗
PubMed1992

[Isolation of Dirofilaria repens in vitreoretinal findings].

The authors present the case of a 18-year-old patient who reported deteriorated vision and mobile dark shadows on the left eye. Examination revealed in the vitreous body an extremely mobile filiform, worm-like formation, white in colour, of the size of an arteriole, about 9 mm long. In the course of hospitalization the formation was isolated by surgery of the pars plana vitrectomy and subjected to detailed helminthological examination. The worm was identified as Dirofilaria repens (Reillet et Henry 1911) and described in detail. The presented finding is the first finding of this parasite in this country and at the same time the first finding of this parasite in Czechoslovakia.

Adolescent↗
PubMed1992

[The argon laser in the prevention of retinal detachment].

In a group of 57 eyes in 51 patients the authors analyze seven years' experience with the use of an argon laser in the prophylaxis of detachment of the retina. The advantages of the argon laser were most marked in traumatic ruptures of the retina and in peripheral vitreoretinal degenerations. In eyes with not closed ruptures after episcleral filling argon laser coagulation proved successful in 77%.

Adolescent↗