[Acetylcholine receptor in skeletal muscle (author's transl)].
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Biomedical subjects
Publications and source records attributed to D Kosk-Kosicka.
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A number of equilibrium and kinetic measurements are presented to characterize the partial reactions of the ATPase and transport cycle in sarcoplasmic reticulum vesicles. The cycle begins with calcium and nucleotide binding on sites available on the outer surface of the vesicles. A phosphorylated enzyme intermediate is then formed, and the calcium sites are subjected to a change in their orientation and their affinity for calcium. It is shown that steps involved in calcium release on the inner side of the vesicles are rate limiting for the cycle, and are followed by hydrolytic cleavage of the intermediate with release of inorganic phosphate and recycling of the enzyme.
An analytical method was elaborated for determination of blood zinc and magnesium based on previously known methods. Blood zinc and magnesium were determined in whole stored blood, plasma and erythrocyte mass. The results obtained by this method were compared with the results of spectrophotometry of atomic absorption (SAA). They showed a rise in the plasma zinc and magnesium levels in blood stored with ACD at +4 degrees -- +6 degrees C. during three weeks.