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D Kozáková

Publications and source records attributed to D Kozáková.

7 recordsLinked to original sources
PubMed2000

Prolactin levels and autoantibodies in female patients with systemic lupus erythematosus.

We investigated the relationships between prolactin (PRL) levels and antibody occurrence in systemic lupus erythematosus (SLE). No significant association between PRL levels and the majority of the autoantibodies studied (anti-U1 RNP, anti-rRNP, anti-Sm, anti-dsDNA, anti-DNP, auto-LCA, anti-EACA) could be confirmed (P > 0.05), anti-Ro/SSA antibodies being an exception. Our results showed significantly increased frequencies of these antibodies in the group of female SLE patients with normal PRL levels (< 20 micrograms/L): anti Ro/SSA in 53% (P < 0.025, chi 2 = 5.80, RR = 4.0) and anti-Ro/SSA + anti-Ro/La in 60% (P < 0.05, chi 2 = 4.05) compared with female SLE patients with hyperprolactinemia.

Adult↗
PubMed1997

Identification of Borrelia burgdorferi sensu lato tick isolates from Slovakia by PCR typing with 16S rRNA primers.

The first four strains of Borrelia burgdorferi isolated in Slovakia from ticks and mice were studied using monoclonal antibodies, the polymerase chain reaction (PCR) with 16S rRNA specific primers and plasmid profiles. Two tick isolates were typed as Borrelia garinii, one strain isolated from Apodemus flavicollis was found to be B. afzelii and the fourth tick isolate reacted as a mixed culture of B. garinii and B. afzelii. All four strains harboured several plasmids ranging from 6-50 kbp including a plasmid with a size of approximately 41 kbp.

Animals↗
PubMed1997

[Preliminary identification of Borrelia burgdorferi isolated from Ixodes ricinus ticks in eastern Slovakia].

The first finding of spirochetes Borrelia burgdorferi sensu lato in eastern Slovakia pertains to ticks Ixodes ricinus collected in the Kosice park forest in September 1995. The authors tested 7 strains using immunoblotting with 4 monoclonal antibodies (Mab): H9724--specific to the genus Borrelia, identifying 41 kDa flagellar protein, H5332--specific to OspA protein of B. burgdorferi, J 8.3 and I 17.3 specific to OspA and OspB proteins of B. afzelii. All the strains belong to the genus Borrelia (reacted with Mab H9724), 6 of 7 strains designated Ir103, Ir105, Ir107, Ir108, Ir112 and Ir113 were identified as B. burgdorferi sensu lato (reacted with Mab H5332). None of the strains reacted with Mab specific for B. afzelii.

Animals↗
PubMed1990

[Detection of antibodies to submitochondrial particles, F1-ATPase complex and liver specific lipoprotein in liver diseases using ELISA].

ELISA was elaborated for the determination of antibodies against liver antigen complexes--submitochondrial particles, F1-ATPase and liver specific lipoprotein. The parameters achieved so far allow to use the assay as an undemanding complementary laboratory technique in diagnosing and monitoring hepatopathies of autoimmune origin. Cross reactivity between individual antigen complexes was recorded in the majority of sera from positively reacting patients. The preliminary results show that individual antigen complexes have similar antigen structures bound to high-molecular membrane complexes, some of which, however, can be solubilized on maintaining antigen activity in ELISA.

Animals↗
PubMed1989

[Antibodies to Klebsiella pneumoniae in ankylosing spondylitis].

The authors investigated the incidence of antibodies against Klebsiella pneumoniae and E. coli in a group of patients with ankylosing spondylitis and in a group of healthy controls in all main immunoglobulin classes. The results revealed that in patients with ankylosing spondylitis there is a significantly higher incidence of specific antibodies of class IgA against Klebsiella pneumoniae and E. coli. This supports the thesis on the aetiopathogenetic interrelationship between enteric bacteria and the development of ankylosing spondylitis.

Adult↗
PubMedDate not supplied

[Systemic lupus erythematodes].

Systemic lupus erythematodes (SLE) is chronic autoimmune disease, characteristic by production of autoantibodies against different autoantigens. Etiopathogenesis in not precise determinated, but genetic, immunologic, hormonal factors or influence of environment are assumed. It manifests by various symptoms and it can affect whichever organ or system in the body. Clinical manifestation are due chronic inflammation in the tissues, which is caused first of all by deposit of immunocomplex and by cytotoxic damage. At the last decades the mortality of patients with SLE is markly lower and their live is prolong. In spite of this diagnostic, to follow up and therapy of this disease is complicated and it requires the colaboration of more branches of medicine.

Humans↗