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Biomedical subjects

D Krüger

Publications and source records attributed to D Krüger.

7 recordsLinked to original sources
PubMed1992

Gastrointestinal transit and digestibility of maltitol, sucrose and sorbitol in rats: a multicompartmental model and recovery study.

Using data obtained with a dye marker and the gavage technique, the kinetics of gastrointestinal transit of different loads of sugar substitutes (maltitol, sorbitol) and sugar (sucrose) in the rat were analysed using a linear multicompartmental model over a range from the realistic to the non-physiologic high, of carbohydrate intake levels and using only a few experimental time points. The model gave detailed insight into intestinal propulsion and gastrocecal transit time. Rate constants of transport between the compartments investigated were determined; they showed characteristics which could be related to the substance and the dosage administered. Analyses of the gastrointestinal content and calculations of the intestinal net water movement showed that the digestibility and absorption of the disaccharide sugar alcohol, maltitol, in the small gut depended inversely on the dose ingested. For all substances tested, caloric availability in the small intestine was calculated. At a physiological low level of maltitol intake, the results also indicated an insignificant calorie-saving effect in comparison to sucrose, an effect based mainly on the slow absorption rate of the maltitol cleavage product sorbitol.

Animals↗
PubMed1992

Carbonic anhydrase in skeletal and cardiac muscle from rabbit and rat.

We have studied the distribution of carbonic anhydrases (CA) in several skeletal muscles of the hindlimb of rabbits and rats and in cardiac muscle of the rabbit. To remove erythrocyte CA, hindlimbs and hearts were thoroughly perfused with dextran solution, and the effectiveness of the perfusion was in most cases assessed by determining the contamination of the muscles with radioisotopes that had been used to label the erythrocytes before the perfusion was started. We observed three forms of CA: (1) cytosolic (sulphonamide-resistant) CA III; (2) a cytosolic sulphonamide-sensitive CA, probably isoenzyme II; (3) a membrane-bound form that was extracted from the particulate fraction using Triton X-100. These CA isoforms were distributed as follows. (1) CA III is located in the cytoplasm of slow, oxidative skeletal muscles and is absent from or low in fast skeletal and cardiac muscle; this holds for rabbits and rats and is identical with the pattern previously described for several other species. (2) The cytosolic sulphonamide-sensitive CA is present in fast rabbit muscles and absent from slow muscles of this species. In contrast, all skeletal muscles of the rat studied here lack, or possess only very low, activity of this isoenzyme. (3) The membrane-bound form of CA is present in all rabbit muscles studied; its activity appears somewhat higher in fast than in slow skeletal muscles. (4) Cardiac muscle constitutes an exception among all striated muscles of the rabbit as it possesses no form of cytosolic CA but a high activity of the membrane-bound form.

Animals↗
PubMed1976

[Results of anti-Rh-immunoprophylaxis in the Berlin-Friedrichshain hospital].

At the beginning the essential facts with a basic signification for the insight into the anti-Rh-immunoprophylaxis (IP) are pointed aut. Then we inform about our own results. During the period--we report on--we carried through the IP nearly 500 times. A sensibilization took place in no case. More than 50 women became pregnant anew, their children were clinically and serologically healthy, no mother proved to have anti-D-antibodies. Herewith our results confirm a full efficiency of the IP. The problem of an immunoprophylactic therapy in already existing anti-D-antibodies is point aut.

Erythroblastosis, Fetal↗
PubMed1975

[Pyrogen testing in vitro using the Limulus test].

The applicability of the Limulus test for the pyrogen test was checked in comparison to the pyrogen test in rabbits. In 6 out of 24 lots of raw materials and drugs pyrogens could be detected by means of the pyrogen test in rabbits. 2 of these 6 lots showed positive reaction in the Limulus test, there were no false positive results. Testing 7 bacterial strains in modified quantity of germs the Limulus test turned out to be more sensitive than the pyrogen test in rabbits. The application of this in vitro test as a complement to the pyrogen test in rabbits for a certain kind of problems is discussed.

Animals↗
PubMed1975

[Immunopreventive management of Rh-positive maltransfusions].

We report on a case of a Rh-positive mis-transfusion in a Rh-negative pregnant woman treated by human-immunoglobulin-G-anti-D (IgG-anti-D). A sensibilization that without a therapy was to be expected with a probability of 75 per cent did not take place. We discuss our observation in connection with information existing hitherto on similar events. Hereby a distinct dependence on an anti-D-dosis becomes evident in order to achieve a reliable immunosuppression in an appointed quantity of erythrocytes. A summarizing of literature at hand results in the fact that a reliable prevention of sensibilization may be achieved by a dosage of 20 microgramme IgG-anti-D each ml of a concentrate of erythrocytes respectively 10 microgramme each ml full blood. This means for a large foetomaternal as well as for false transfusions of adult blood. The dosage in the case, described by us, therefore by 1,9: 1 related to full blood was too low. We acted perfectly consciously as up to date there are no informations on a treatment by anti-D in this way during pregnancy and because an eventually Rh-positive child should not be injured at all. The clinical course after the anti-D-treatment has been examined by numerous authors. It showed even in a therapy by high doses of great quantities of erythrocytes but slight reactions. Severe disorders have not been described hitherto. We therefore ought to treat by sufficiently high doses and as soon as possible in order to allow the organism to contact with the intact antigen as brief as possible. Finally we present a proposition on diagnostics and therapy for the case of a Rh-mis-transfusion respectively a large glut in of foetal blood cells in order to avoid an immunization within the Rh-system.

Adult↗