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Biomedical subjects

D Krikorian

Publications and source records attributed to D Krikorian.

4 recordsLinked to original sources
PubMed2001

Structure of epitopes recognized by the antibodies to alpha(181-192) peptides of neuronal nicotinic acetylcholine receptors: extrapolation to the structure of acetylcholine-binding domain.

Using the alpha(181-192) peptides of neuronal nicotinic acetylcholine receptor (nAChR) and Ala-substituted peptide analogues, amino acid residues critical for specific monoclonal antibody (mAb) binding were identified. By means of 2D nuclear magnetic resonance (2D-NMR) analysis followed by molecular modeling, it was found that mAb binding resulted in stabilization of the free alpha3(181-192) peptide flexible conformation yielding an extended structure with residues 6-11 of the peptide being in direct contact with the Ab. Since the Ab binds the native AChR as well, it is suggested that the corresponding fragment of AChR alpha3 subunit is exposed to solution and also appears in extended conformation.

Alanine↗
PubMed1989

Effect of L-lysine monohydrochloride on cutaneous herpes simplex virus in the guinea pig.

The effect of topical applications of crystalline lysine therapy on cutaneous herpes simplex virus (HSV) inoculations and subsequent dorsal root ganglia (DRG) infection was studied in male Hartley guinea pigs. Although HSV-I was recovered from the inoculated sites from all animals, the L-lysine-treated skin remained clinically normal, whereas untreated controls manifested clinical symptoms up to 3 days postinoculation (p.i.). However, cocultivation of DRG (C1-S1) indicated a selective tropism of infective particles to specific DRG in the groups treated with amino acids. In lysine-treated animals, HSV was recovered from a few DRG (T-12, T-13, and L-1) at 3 days p.i. and from DRG T-10 in leucine-treated controls; yet no HSV was recovered from DRG of untreated controls. These results suggest an immunomodulatory effect of L-lysine on inoculation site infections and the possible potentiation of subsequent DRG manifestation in amino-acid-treated animals.

Administration, Topical↗
PubMed1982

Purified mouse Schwann cells: mitogenic effects of fetal calf serum and fibroblast growth factor.

We present an in vitro bioassay protocol for the detailed examination of mitogenic influences on purified mouse Schwann cells. This involves measurements of (i) increases in Schwann cell number per culture, (ii) incorporation of radiolabeled thymidine per culture, and (iii) the proportion of total Schwann cells that exhibit a labeled nucleus by autoradiography. Using this standard protocol we show that the mitogenic efforts of fetal calf serum must be distinguished from its effects on cell retention to the culture substratum, and preparations of mouse brain or pituitary fibroblast growth factor but not mouse epidermal growth factor are very potent mouse Schwann cell mitogens. These mitogenic effects of serum and fibroblast growth factor have not been reported for rat Schwann cells.

Animals↗