Biomedical subjects

D L Carnes

Publications and source records attributed to D L Carnes.

At least 37 records · Page 2Linked to original sources

PubMed1999

Evaluation of apical seal in straight canals after obturation using the Lightspeed sectional method.

This study evaluated the sectional gutta-percha obturation technique advocated by Lightspeed Technology, Inc. Single relatively straight-canaled teeth were prepared with rotary instruments and divided into three groups. Groups A and B served as controls and were obturated using laterally condensed gutta-percha and either Roth's 801 or Ketac-Endo sealer. Group C was obturated using the Lightspeed technique that included placement of a 5-mm apical section of gutta-percha, followed by backfill with Ketac-Endo sealer and a single gutta-percha cone. All teeth were suspended in India ink for 14 days then cleared. Four additional teeth, which were obturated as in group C then sectioned, revealed a tightly adapted apical section of gutta-percha with a very thin layer of sealer. However, voids were noted in the middle and coronal areas. There was no significant difference in apical microleakage among the three groups. The sectional method was significantly faster than lateral condensation and seems to offer promise as an effective obturation method.

Chi-Square Distribution↗

PubMed1999

Longitudinal microleakage evaluation of Super-EBA as a root-end sealing material.

This study evaluated the sealing ability of fast and regular set Super-EBA with different powder-to-liquid ratios as a root-end filling material. Fifty extracted maxillary central incisors were uniformly cleaned and shaped using a crown-down technique with Gates-Glidden drills and Profile series 29.04 taper rotary instruments. After root-end resection and ultrasonic preparation, 48 root sections were randomly assigned to 4 groups of 12 teeth each and filled with a thick or thin mix of fast or regular set Super-EBA. Fast and regular set Super-EBA were mixed to a thick (1 scoop powder:1 drop liquid) or a thin (1 scoop powder:2 drops liquid) consistency. Positive and negative controls were used. Microleakage was assessed at 24 h, 72 h, 1 wk, 2 wk, 4 wk, 6 wk, and 8 wk using a fluid filtration system. There were no significant differences in the microleakage measured for any group at any of the time points evaluated. These results suggest that either fast or regular set Super-EBA mixed to various consistencies may be acceptable for root-end filling.

Analysis of Variance↗

PubMed1999

BMP stimulation of bone response adjacent to titanium implants in vivo.

The objective of this study was to evaluate the effect of bone morphogenetic protein (BMP) on the bond strength of titanium (Ti) implants at the bone-implant interface. Thirty-six Brånemark screw implants (3.75 mm diameter by 15 mm long) were implanted for 3 and 12 weeks. At 3 weeks after implantation, the mean reverse torque value for implants stimulated with BMP delivered using an atelopeptide type-I collagen carrier (74.2 +/- 5.2 N cm) was observed to be statistically greater (P < 0.000016) than the mean reverse torque value for control Ti implants (32.8 +/- 1.1 N cm). Similarly, at 12 weeks after implantation, the mean reverse torque value for implants stimulated with BMP (89.2 +/- 2.7 N cm) was statistically greater (P < 0.0042) than the mean reverse torque value for control Ti implants (75.8 +/- 2.4 N cm). In addition, histomorphometric evaluations indicated more bone contact with the BMP stimulated implant surfaces compared to the controls after 3 weeks implantation. It was concluded from this study that the use of BMP-atelopeptide type-I collagen mixture is an effective means of obtaining greater bond strength at the bone implant interface within a shorter time period than Ti implants without BMP.

Animals↗

PubMed1999

Evaluation of 2 novel approaches for assessing the ability of demineralized freeze-dried bone allograft to induce new bone formation.

BACKGROUND: Because of the wide variation in the ability of human demineralized freeze-dried bone allograft (DFDBA) to reproducibly induce new bone formation, there is a need for a reliable measure of bone induction activity. In this study we examined an immature osteoprogenitor cell line for its potential utility in measuring the activity of DFDBA in vitro. METHODS: We characterized the response of 2T9 cells, an immature osteoprogenitor cell line derived from the calvariae of transgenic mice containing the SV40 T-antigen driven by the mouse bone morphogenetic protein (BMP)-2 promoter, to recombinant human BMP-2 by measuring alkaline phosphatase specific activity, osteocalcin production, and matrix mineralization. Responses were compared to those obtained with 1,25-(OH)2D3. In addition, 2T9 cells were cultured with active or inactive human DFDBA in the presence or absence of BMP-2. We also tested the hypothesis that radio-opacity of tissue following implantation of DFDBA in vivo correlates with the ability of human DFDBA to induce new bone. DFDBA from 9 different donors, stratified by age, were implanted subcutaneously in the thorax of 18 nude (nu/nu) mice. Tissue was harvested at 36 days postoperatively and examined histologically and biochemically for calcium and phosphorus uptake. RESULTS: 2T9 cells exhibited a dose- and time-dependent response to soluble BMP-2. Proliferation was decreased and alkaline phosphatase activity, osteocalcin production, and mineralized nodule formation were increased. The effects were dose- and time-dependent. Peak effects on alkaline phosphatase and osteocalcin were noted on day 8, whereas mineral deposition did not begin to occur until day 12. 1,25-(OH)2D3 did not regulate these effects unless used with BMP-2. When the cells were exposed to active or inactive DFDBA in the presence or absence of BMP-2, no effect on 2T9 cell differentiation was observed. This indicated that DFDBA released no soluble factors with bone inductive ability and that if any active factors were adsorbed to the DFDBA, they were inactivated. When DFDBA was implanted subcutaneously in the thorax of nude mice, there was no histologic evidence of new bone formation. However, there was a donor age-dependent decrease in Ca and P uptake of the implanted tissue, reflecting a donor age-dependent decrease in remineralization of DFDBA. CONCLUSIONS: These data indicate that cell culture assays like the one used in this study may not be appropriate indicators of bone induction ability by DFDBA since soluble factors may not be responsible for bone induction in vivo. Nonetheless, in vitro assays are still needed. While Ca and P uptake by DFDBA-implanted tissue in the present study correlated with the age-dependent decrease in bone induction at intramuscular sites in a previously reported study, these data show that early x-rays may actually detect remineralization and not new bone formation. Thus, assessment of bone induction ability may still depend on histologic analysis of animal models.

Age Factors↗

PubMed1998

Osteoblast precursor cell activity on HA surfaces of different treatments.

The clinical success of dental implants is governed by implant surfaces and bone cell responses that promote rapid osseointegration and long-term stability. The specific objective of this study was to investigate osteoblast precursor cell responses to hydroxyapatite (HA) surfaces of different treatments. Since the nature of bone cell responses in vitro is influenced by the properties of HA ceramics, this study was divided into two components: a chemical and crystallographic characterization of the HA ceramics and an in vitro cell culture study. The sintered HA samples were observed to have the highest crystallite size as compared to the as-received HA and calcined HA samples. No differences in the surface roughness and chemical composition were observed among the sintered, calcined, and as-received HA surfaces. In concurrence with the X-ray diffraction, high resolution XPS resolution of Ca 2p also indicated a higher crystallinity on sintered HA samples as compared to the calcined and as-received HA samples. As indicated by increased alkaline phosphatase-specific activity, increased cell-surface and matrix-associated protein, and 1.25 (OH2) vitamin D3-stimulated osteocalcin production, a more differentiated osteoblast-like phenotype was observed on the sintered HA surfaces compared to the as-received HA and calcined HA surfaces. An increased osteoblast-like cell activity on the sintered HA surfaces suggested that the crystallite size of HA surfaces may play an important role in governing cellular response.

Alkaline Phosphatase↗

PubMed1998

Inflammation-associated lysyl oxidase protein expression in vivo, and modulation by FGF-2 plus IGF-1.

Lysyl oxidase is the extracellular enzyme that catalyzes oxidative deamination of peptidyl-lysine residues in elastin precursors, and lysine and hydroxylysine residues in collagen precursors to form peptidyl-aldehydes. These aldehydes then spontaneously condense to crosslink collagen and elastin and thereby allow the formation of a mature and functional extracellular matrix. In the present study, cryosections made from aseptic immune-induced periapical lesions experimentally generated in laboratory rats were examined by immunohistochemistry to investigate whether lysyl oxidase protein expression is altered in inflamed oral tissues. Periapical lesions are experimentally induced endodontic lesions of tooth roots. In addition, the effect of administration of a mixture of fibroblast growth factor (FGF)-2 and insulin-like growth factor (IGF)-1 into these lesions on lysyl oxidase expression was determined. Lysyl oxidase expression was found to be increased in non-mineralized connective tissue adjacent to inflamed lesions. Morphometric analyses indicated that maximum lysyl oxidase expression occurred at a discrete distance from the lesion not exceeding 350 microm from the inflammatory cells. Staining was associated with mesenchymal cells with a fibroblastic morphology. No lysyl oxidase staining was found near teeth where no lesion was induced. Application of a mixture of FGF-2 and IGF-1 resulted in a further twofold increase in lysyl oxidase expression. These results provide a new in vivo model to study lysyl oxidase regulation, and suggest that inflammatory cells may control lysyl oxidase expression in oral tissues, possibly by a mechanism involving secretion of cytokines and other factors, probably contributing to the regulation of extracellular matrix accumulation.

Animals↗

PubMed1998

Effect of sterilization on cyclic fatigue of rotary nickel-titanium endodontic instruments.

The ability of heat treatment as a result of autoclave sterilization to extend the life of nickel-titanium rotary endodontic instruments by reducing the effect of cyclic fatigue was evaluated using 280 size 40 Lightspeed instruments. Instruments were cycled in artificial canals with angles of curvature of 30 degrees and either 2 or 5 mm radii of curvature. In a pilot study, instruments were sterilized or not sterilized and cycled to failure to obtain mean cycles-to-failure values for each group. In the first experimental protocol, instruments were cycled to either 25%, 50%, or 75% of the mean cycles-to-failure limit determined in the pilot study, then sterilized or not sterilized before being cycled to failure. In the second experimental protocol, instruments were cycled to 25% of the mean cycles-to-failure determined in the pilot study, and sterilized or not sterilized. The sequence of cycling to 25% of the predetermined cycles-to-failure limit followed by sterilization was repeated until the instruments failed. No significant increases in cycles to failure were observed between groups for either experimental protocol when instruments were evaluated at a similar radius. Significant differences in cycles to failure were only observed when instruments cycled to failure in the artificial canal with 2 mm radius were compared with instruments cycled to failure in the artificial canal of 5 mm radius. Scanning electron microscopic photos showed crack initiation and propagation in all instruments that were cycled to a percentage of the predetermined cycles-to-failure limit. It is concluded that heat treatment as a result of autoclave sterilization does not extend the useful life of nickel-titanium instruments.

Analysis of Variance↗

PubMed1998

Microleakage of TERM used with sodium perborate/water and sodium perborate/superoxol in the "walking bleach" technique.

This study compared the sealing ability of TERM as an interim restoration for the "walking bleach" technique when using either sodium perborate/water or sodium perborate/superoxol. Thirty-three extracted maxillary premolars were restored with TERM after placing either a cotton pellet (control), a paste of sodium perborate and water, or a paste of sodium perborate and superoxol in the chamber. Microleakage was assessed at 24 h, 8 days, and 15 days using a fluid filtration method. Statistical analysis revealed both "walking bleach" groups had significantly higher numbers of teeth demonstrating microleakage than the control group. There was no statistically significant difference in microleakage between the two "walking bleach" groups. The results of this study indicated that the temporary restorative material, TERM, provided an unsatisfactory seal when used with either walking bleach technique. When used over a cotton pellet, TERM provided an excellent seal.

Borates↗

PubMed1998

Addition of human recombinant bone morphogenetic protein-2 to inactive commercial human demineralized freeze-dried bone allograft makes an effective composite bone inductive implant material.

COMMERCIAL PREPARATIONS OF HUMAN DEMINERALIZED freeze-dried bone allograft (DFDBA) vary in their ability to induce new bone formation. This study tested the hypothesis that inactive DFDBA can be used as an effective carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2). Two batches of active DFDBA were used as controls. Two batches of DFDBA, previously shown to be inactive, were treated with vehicle or with 5 or 20 microg rhBMP-2 and implanted into the calf muscle of male Nu/Nu (nude) mice. Each mouse received one implant in each hind limb, both of which were of the same formulation, resulting in 8 groups of 4 mice per group: active DFDBA batch A, active DFDBA batch B, inactive DFDBA batch A, inactive DFDBA batch B, inactive DFDBA batch A plus 5 microg rhBMP-2, inactive DFDBA batch A plus 20 microg rhBMP-2, inactive DFDBA batch B plus 5 microg rhBMP-2, and inactive DFDBA batch B plus 20 microg rhBMP-2. After 56 days, the implants were removed and histologically examined. A semiquantitative bone induction index was calculated based on the amount of new bone covering each histological section. Histomorphometry was also used to evaluate the area of new bone formed and the area of residual implant material. The results showed that active DFDBA induces new bone formation, whereas inactive DFDBA does not. Addition of rhBMP-2 to inactive DFDBA results in new bone formation with a bone induction index comparable to that of active DFDBA. Histomorphometric analysis, however, revealed that the rhBMP-2-containing implants caused a dose-dependent increase in new bone area that exceeded that induced by active DFDBA. At the highest concentration of rhBMP-2, bone formation was exuberant. rhBMP-2 also caused the resorption of residual implant material to levels comparable to that seen in sites treated with active DFDBA, suggesting that this growth factor may regulate resorptive cells either directly or indirectly. This study shows that addition of rhBMP-2 to inactive DFDBA provides reproducible, consistent bone induction, and suggests that inactive commercial preparations may contain inadequate amounts of BMP to cause bone induction compared to active preparations.

Animals↗

PubMed1998

Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation is dependent on donor age but not gender.

Demineralized freeze-dried bone allografts (DFDBA) have been used extensively in periodontal therapy. DFDBA is used because it contains bone morphogenetic protein (BMP), which induces new bone formation during the healing process. Most commercial bone banks do not verify the presence or activity of BMP in DFDBA nor the ability of DFDBA to induce new bone. Recently, we showed that different bone bank preparations of DFDBA, even from the same bank, varied considerably in their ability to induce new bone, suggesting inherent differences in the quality of the material. Therefore, we examined whether donor age or gender contributed to the variability seen with these preparations. Twenty-seven batches of DFDBA from different donors were donated by one bone bank which had been shown previously to supply DFDBA that was consistently able to induce new bone formation. Each batch was implanted bilaterally in the thigh muscle of nude mice. After 56 days, the implants were excised and examined by light microscopy and histomorphometry. Seventy percent of the preparations tested induced new bone formation. Most of these preparations produced ossicles containing cortical bone surrounding bone marrow-like tissue. The ability to induce bone appears to be age-dependent, with DFDBA from older donors being less likely to have strong bone-inducing activity. By contrast, no difference in ability to induce new bone was noticed between male or female donors. The results of this study confirm that commercial preparations of DFDBA differ in their ability to induce new bone formation. In fact, some of the batches had no activity at all. The ability of DFDBA to induce new bone formation is suggested to be age-dependent, but not gender-dependent by our study. These results indicate that commercial bone banks need to verify the ability of DFDBA to induce new bone formation and should reconsider the advisability of using bone from older donors.

Adolescent↗

PubMed1998

Immortalized mouse odontoblast cell line MO6-G3 application for in vitro biocompatibility testing.

PURPOSE: This study was designed to determine the usefulness of an established stable immortalized mouse odontoblast cell line (MO6-G3) for dental material biocompatibility testing. Using a standard toxicity assay based on cell respiratory activity, the response to MO6-G3 cells was compared to the mouse fibroblastic cell line, L929, presently used for dental materials testing. The dental resin monomer TEGDMA was used as the dental material for the assay. MATERIALS AND METHODS: Cell lines (1 x 10(3)/well) were plated in 96 well culture plates and grown in DMEM supplemented with 10% FCS, 100 units/ml each of penicillin and streptomycin, and 50 micrograms/ml ascorbic acid in an atmosphere of 95% air and 5% CO2. Cells were exposed to TEGDMA resin monomer covering a dose range of 1 x 10(-6) to 0.5 x 10(-3) M. Unexposed control cells, as well as cells exposed to the DMSO vehicle in which the TEGDMA was dissolved, were included in all assays. Cytotoxicity was evaluated by determining cell respiratory activity spectrophotometrically using the tetrazolium compound WST-1. RESULTS: Statistical analysis by ANOVA using Tukey's method for pair wise comparisons as the post hoc test indicated toxic effects of TEGDMA at 1 x 10(-5) M in the odontoblast cell line MO6-G3. By contrast, the monomer produced no toxic effects on the L929 fibroblast cell line after 24 hours of exposure, over the entire concentration range tested. Furthermore, MO6-G3 cells exposed to a concentration of 0.5 x 10(-3) M were unable to recover from the effects of the exposure 48 hours after removal of the resin. MO6-G3 cells exposed to 1 x 10(-4) and 0.5 x 10(-4) TEGDMA recovered 40-50% and 75-80% of control respiratory activity respectively, 48 hours after removal of the resin. Respiratory activity by L929 cells exposed to all TEGDMA concentrations tested was not different from the vehicle control 48 hours after removal of the resin.

Analysis of Variance↗

PubMed1997

Cyclic fatigue testing of nickel-titanium endodontic instruments.

Cyclic fatigue of nickel-titanium, engine-driven instruments was studied by determining the effect of canal curvature and operating speed on the breakage of Lightspeed instruments. A new method of canal curvature evaluation that addressed both angle and abruptness of curvature was introduced. Canal curvature was simulated by constructing six curved stainless-steel guide tubes with angles of curvature of 30, 45, or 60 degrees, and radii of curvature of 2 or 5 mm. Size #30 and #40 Light-speed instruments were placed through the guide tubes and the heads secured in the collet of a Mangtrol Dynamometer. A simulated operating load of 10 g-cm was applied. Instruments were able to rotate freely in the test apparatus at speeds of 750, 1300, or 2000 rpm until separation occurred. Cycles to failure were determined. Cycles to failure were not affected by rpm. Instruments did not separate at the head, but rather at the point of maximum flexure of the shaft, corresponding to the midpoint of curvature within the guide tube. The instruments with larger diameter shafts, #40, failed after significantly fewer cycles than did #30 instruments under identical test conditions. Multivariable analysis of variance indicated that cycles to failure significantly decreased as the radius of curvature decreased from 5 mm to 2 mm and as the angle of curvature increased greater than 30 degrees (p < 0.05, power = 0.9). Scanning electron microscopic evaluation revealed ductile fracture as the fatigue failure mode. These results indicate that, for nickel-titanium, engine-driven rotary instruments, the radius of curvature, angle of curvature, and instrument size are more important than operating speed for predicting separation. This study supports engineering concepts of cyclic fatigue failure and suggests that standardized fatigue tests of nickel-titanium rotary instruments should include dynamic operation in a flexed state. The results also suggest that the effect of the radius of curvature as an independent variable should be considered when evaluating studies of root canal instrumentation.

American Dental Association↗

PubMed1997

Oral bone loss is increased in ovariectomized rats.

Alveolar bone loss associated with periodontal disease occurs frequently in postmenopausal females, the same group that is predisposed to osteoporosis. To determine if the estrogen-deficient state enhances oral bone loss, we studied ovariectomized rats administered the potent bone-resorbing cytokine interleukin-1 or the periodontal pathogen Campylobacter rectus lipopolysaccharide (LPS). Distal root canals of first mandibular molars were instrumented with endodontic files, and bone resorbing factors were deposited and sealed into the root canal. Radiographs of periapical bone loss were evaluated using computer assisted image analysis to determine lesion size. Both interleukin-1 and C. rectus LPS caused a significant increase in lesion area in both ovariectomized and normal rats when compared with controls and a significant increase in ovariectomized animals compared to nonovariectomized animals receiving LPS. Using this endodontic model, we have demonstrated that estrogen deficiency results in increased oral bone loss in rats.

Alveolar Bone Loss↗

PubMed1997

Human pulp cells respond to calcitonin gene-related peptide in vitro.

Cell monolayers derived from human pulpal explants were passaged 3 to 4 times before characterization of the response of the cells to calcitonin gene-related peptide (CGRP). Northern blot analysis of mRNA revealed the presence of transcripts for bone morphogenetic protein-2 (BMP-2). Stimulation with CGRP produced a 1.8-fold increase in BMP-2 mRNA expression by the cells. Analysis of binding of CGRP to whole cells indicated that unlabeled human CGRP competed with labeled CGRP in a dose dependent fashion with a KD, estimated from the EC50, in the range of 5 x 10(-8)M. Binding of labeled CGRP was greatly reduced by the presence of 10(-6)M CGRP but was unaffected by the presence of 10(-6)M Human PTH(1-34) or 10(-6)M calcitonin. CGRP produced a 2.8-fold increase in cyclic AMP over basal levels, which was similar to the increase produced by PTH(1-34) (2.6 fold) but slightly more than the increase produced by calcitonin (1.9 fold). The pulp-derived cells displayed a high basal level of alkaline phosphatase enzyme activity, which was not altered by treatment with CGRP or either PTH(1-34) or 1,25(OH)2D3. Stimulation with 2.5 x 10(-8)M 1,25(OH)2D3 did produce a 7.6-fold increase in osteocalcin. These results indicate that pulp cells possess the cellular machinery to respond to CGRP and that stimulation of the production of BMP-2, a factor known to be associated with induction of dentin formation, is a component of the response.

Blotting, Northern↗

PubMed1997

Effect of tip design of nickel-titanium and stainless steel files on root canal preparation.

The purpose of this study was to evaluate the effect of modified and non-modified tip designs of both stainless steel and nickel-titanium endodontic hand files on root canal preparation. Root canals of mesial roots of extracted mandibular molars were prepared using a quarter-turn-pull technique. The experimental design permitted comparison of the effects of nickel-titanium alloy construction or file tip modification alone, with effects when these two factors were incorporated into a single file design. Transportation, centering ratio, and dentin removal were evaluated using a modified Bramante technique. Nickel-titanium files, regardless of tip design, remained significantly more centered and demonstrated less apical transportation than stainless steel files at size 25. When instrumentation was continued to size 40 apically with step-back, there were no significant differences in transportation in the apical or coronal sections. However, during instrumentation to size 40 with step-back, the combination of modified tip and nickel-titanium alloy produced significantly more transportation and dentin removal, as well as greater deviation from the center at the mid-root level than did other file designs. Thus, of the file design parameters evaluated in this study, construction from nickel-titanium was most important in defining canal configuration at small file sizes in the apical region. Although the combination of nickel-titanium and modified tip design resulted in more transportation at larger file sizes in the midroot region, such alterations in canal configuration may be of little importance clinically. Nonetheless, it is suggested that other factors, such as tactile sensation and instrumentation technique, should be considered as important as the type of alloy or tip design.

Analysis of Variance↗

PubMed1997

Determination of periodontal ligament cell viability in the oral rehydration fluid Gatorade and milks of varying fat content.

The purpose of this study was twofold: 1) to determine if the oral rehydration fluid Gatorade could serve as a suitable temporary storage medium for maintenance of periodontal ligament (PDL) cell viability on avulsed teeth and 2) to determine if fat content is related to maintenance of cell viability when milk is used as the temporary storage medium. PDL cells were cultured from extracted human teeth then incubated over timed intervals from 15 to 210 min in the oral rehydration fluid Gatorade, milks of varying fat content, and saliva. Dulbeco's Modified Eagles Medium served as the positive control while tap water served as the negative control. Cell viability was determined using a colorimetric assay that used Cell Proliferation Reagent WST-1. Results using Gatorade yielded cell viability data similar to the negative control, tap water, indicating that this oral rehydration fluid was not suitable as a temporary storage medium for the avulsed tooth. However, the fat content of milk was found to have an effect on cell viability, suggesting that milks with lower fat content may be more appropriate for maintaining PDL cell viability than milks with higher fat content.

Animals↗

PubMed1997

Monocyte chemoattractant protein-1 induces monocyte recruitment that is associated with an increase in numbers of osteoblasts.

Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine family of cytokines. The principal function of MCP-1 is thought to be the stimulation of monocyte recruitment. Monocyte products are potential regulators of bone cell activity. Growth factors produced by monocytes may stimulate bone formation, while cytokines such as IL-1 and IL-6 can induce bone resorption. To determine whether MCP-1 enhances recruitment of monocytes during bone healing, studies were carried out in which MCP-1 was applied to osseous sites in vivo. Changes in monocyte number were determined by immunohistochemistry using the antibody ED-1 specific for peripheral monocytic cells. The effect of MCP-1 on osteoblast number was determined by counting the number of alkaline phosphatase positive cells in close proximity to bone. For comparison, osteoblast number was also determined following stimulation with platelet-derived growth factor (PDGF)-BB plus IGF-1 in vivo. Results indicate that MCP-1 stimulated a large increase in monocyte recruitment compared to vehicle alone. An increase in monocytes induced by MCP-1 was associated with an increase in the number of osteoblasts lining the bone surface, although not to the same magnitude as a positive control, PDGF-BB, and IGF-1. These results indicate that MCP-1 induces the recruitment of monocytes to bone and suggest that the recruitment is associated with an increase in osteoblast number. This is likely to occur via indirect mechanisms, because MCP-1 did not directly enhance DNA synthesis in osteoblastic cells in vitro. Thus, activated mononuclear phagocytes may play an important role in osseous wound healing by stimulating proliferation of osteoblastic cells, presumably through the elaboration of growth factors.

Alkaline Phosphatase↗

PubMed1997

Surface roughness of titanium on bone morphogenetic protein-2 treated osteoblast cells in vitro.

Surface topography plays a critical role in the interaction of dental implants with adjacent tissues. No statistical differences in oxide composition and surface contamination were observed between 600 grit and polished titanium surfaces. The expression of osteoblast phenotype was enhanced when osteoblast progenitor cells (2T9) were stimulated with bone morphogenetic protein-2 on polished and 600 grit titanium surfaces. Bone morphogenetic protein-2 stimulated phenotypic expression on 600 grit titanium surfaces was marked by prolonged alkaline phosphatase specific activity and more rapid osteocalcin production as compared with the polished titanium surfaces. Because the surface area of the 600 grit titanium surface was shown to be 8 percent greater than that of the polished titanium surface, it is possible that increased surface area played a role in the enhanced expression of the osteoblast phenotype.

Alkaline Phosphatase↗