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D L Hopkins

Publications and source records attributed to D L Hopkins.

12 recordsLinked to original sources

Randomly amplified polymorphic DNA analysis of Xylella fastidiosa Pierce's disease and oak leaf scorch pathotypes.

Randomly amplified polymorphic DNA analysis was conducted with 14 primers to 17 strains of Xylella fastidiosa. There was a high degree of similarity among the seven Pierce's disease (PD) strains (Sxy > 0.93) and the seven oak leaf scorch (OLS) strains (Sxy > 0.96). However, the two groups were different, with a similarity index of 0.67, confirming the presence of a PD DNA cluster and suggesting the presence of a new OLS cluster. The control plum leaf scald strains (two strains) together with the periwinkle wilt strain had a much smaller similarity index (0.44) compared with the PD and OLS clusters.

Base Sequence

Birth deformities.

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Abnormalities, Drug-Induced

Production, behaviour and fertility of merino wethers, hemi-castrates with reduced testicular parenchyma and induced cryptorchids.

Greasy wool production, body weight, behaviour and fertility of male, fine wool Merino sheep were studied from 10 to 21 months of age to assess their suitability for wool production. The males were either castrated (wethers), or were hemi-castrated with a vasectomy and reduction (either partially or in full) of the parenchyma of the retained testicle, or the testicles pushed up into the inguinal canal and the scrotum shortened (induced cryptorchids). There were significant differences among all groups in body weight, with increases being associated with the larger amounts of testicular tissue present. Fertility was low in the induced cryptorchids at 19 months of age. The hemi-castrates with reduced testicular parenchyma were infertile. The hemi-castrates with reduced testicular parenchyma had significantly heavier greasy fleece weights than the wethers and induced cryptorchids, which had similar fleece weights. The wether group suffered a higher prevalence of posthitis in spring and autumn than the hemi-castrate or induced cryptorchid groups. Those hemi-castrates with the largest amount of testicular parenchyma retained and the induced cryptorchids exhibited masculine behaviour patterns and had an increased incidence of fly strike to the head. The principle of hemi-castration and reduction of the testicular parenchyma is shown to be applicable in wool producing flocks with the potential to increase greasy wool production while minimising the management and marketing limitations previously associated with induced cryptorchids.

Animals

Ultrastructural study of Pierce's disease bacterium in grape xylem tissue.

The rod-shaped rickettsia-like bacteria of Pierce's disease measure about 0.25 to 0.50 mum in diameter and 1.0 to 4.0 mum long. The bacteria have a cell wall consisting of a trilaminar outer membrane and two intermediate low-density layers separated by a dense intermediate layer. A trilaminar cytoplasmic membrane is also present, resulting in a total wall complex thickness of 25 to 40 nm. A periodic infolding of the outer membrane and intermediate layers of the wall give the wall surface a ridged apperance. The ridges appear to go around the long axis of the cell, possibly in the form of spirals. Ribosomes and nuclear regions with easily visible deoxyribonucleic acid strands and clumps are distributed throughout the cytoplasm. Binary fission, during which the cell wall and cytoplasmic membrane folded inward to partition the cell, was observed. In the xylem of infected grapes, the bacteria are either distributed evenly throughout the lumen of the xylem vessel or appressed along the inner surface of the vessel walls in an electron-lucent matrix.

Cell Membrane