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Biomedical subjects

D L Huxsoll

Publications and source records attributed to D L Huxsoll.

At least 73 records · Page 4Linked to original sources
PubMed1975

Experimentally induced infection of dogs, cats, and nonhuman primates with Ehrlichia equi, etiologic agent of equine ehrlichiosis.

Dogs (German Shepherd Dogs and Beagles), cates, rhesus macaques (Macaca mulatta), and baboons (Papio anubis) were inoculated with Whrlichia equi, the etiologic agent of equine ehrlichiosis. Within 3 to 7 days after inoculation, morulae were observed in the eosinophils of cats, neurtrophils of macaques and baboons, and in both neutrophils and eosinophils of dogs. The severe disease produced in horses by this agent was not a feature of E equi infection in dogs, cats, macaques, and baboons. However, a susceptible horse, inoculated with the pooled blood of 2 infected macaques, developed severe clinical signs of equine ehrlichiosis. Infection with E equi did not alter the susceptibiltiy of dogs to infection with Ehrlichia canis and did not prevent development of signs of disease resulting from this infection. The broad experimental host range from this infection. The broad experimental host range of E equi distinguishes it from other ehrlichial agents which are characterized by a rather narrow host range. The susceptibiltiy of nonhuman primates to infection with E equi provides a basis for consideration of the potential transmission of ehrlichial agents to man.

Animals↗
PubMed1975

Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus).

Two strains of Rhipicephalus sanguineus acquired Ehrlichia canis by feeding as either larvae or nymphs on acutely infected dogs and, in subsequent instars, transmitted the agent to normal dogs. Three strains of R sanguineus transmitted E canis as adults after their larval and nymphal stages fed on infected dogs. More than 400 adult female ticks were fed on infected dogs as larvae or nymphs or both, but none transmitted E canis transovarially.

Alanine Transaminase↗
PubMed1975

Kinetics of antibody response to Ehrlichia canis assayed by the indirect fluorescent antibody method.

The kinetics of antibody production response to experimentally induced infection of dogs with Ehrlichia canis was determined by ion-exchange and molecularsieve chromatography and by indirect fluorescent antibody (IFA) test. The first IFA antibody at 7 days after inoculation resided in immunoglobulin M (IgM) and immunoglobulin A (IgA) classes. At approximately 21 days after inoculation, the antibody was in IgM, IgA, and immunoglobulin G (IgG) classes. Thereafter, antibody concentrations continued to increase in the IgG class; those in the other 2 immunoglobulin classes had a variable pattern. In 2 dogs which died 60 and 114 days after inoculation, a decrease of antibody concentration in the 3 immunoglobulin classes was evident at the time of death. In the carrier dog, however, which was killed 147 days after inoculation, antibody concentrations sustained increasing titers in the 3 immunoglobulin classes.

Animals↗
PubMed1975

Protection against scrub typhus infection engendered by the passive transfer of immune sera.

The passive transfer of convalescent sera did not protect the majority of mice against challenge with the homologous strain and was completely ineffective against challenge with strains unrelated by fluorescent antibody techniques. When the immune sera was incubated with the rickettsia in vitro and then inoculated into the mice a dramatic increase occurred in the number of surviving mice. The importance of these data in relation to published results with other species of rickettsia is discussed.

Animals↗
PubMed1973

Ultrastructure of Ehrlichia canis.

The ultrastructure of Ehrlichia canis was examined in both pulmonary mononuclear cells and in monocytes cultured from an infected dog. The cytoplasmic inclusions, or morulae, of E. canis consisted of a membrane-lined vacuole-containing elementary bodies which varied in size and number. The elementary bodies were bound by two trilamellar membranes. The organism shared morphological properties of both the genus Rickettsia and genus Chlamydia.

Animals↗
PubMed1972

Zoonotic infections in military scout and tracker dogs in Vietnam.

Significant levels of antibodies indicative of a variety of zoonotic infections were demonstrated in sera collected from 64 U.S. military scout and tracker dogs after service in the Republic of Vietnam (RVN). Scrub typhus antibodies were found in 45% of the specimens, melioidosis in 19%, group B arbovirus in 49%, and leptospirosis in 62%. Only 38% of the seropositive reactions for leptospirosis could be related to overseas infections. Tests were conducted on paired sera obtained from 32 of the dogs before service in RVN. Significant increases in titer were demonstrated with scrub typhus, melioidosis, leptospirosis, plague, group B arbovirus, and Rickettsia canada antigens. After 2 to 6 months of service in RVN, 8 of 19 dogs developed antibody titers to at least one of four zoonotic diseases. Only 3 of 45 dogs with 7 or more months of service failed to develop antibodies to one or more of the agents. The serological findings pose questions on the potential epidemiological importance and veterinary significance of scrub typhus, group B arbovirus, leptospirosis, and melioidosis infections in dogs.

Animals↗
PubMed1972

Serological diagnosis of tropical canine pancytopenia by indirect immunofluorescence.

An indirect fluorescent-antibody test for detection and titration of antibodies to Ehrlichia canis, the causative agent of tropical canine pancytopenia, has been described. The organism propagated by an in vitro technique in canine blood monocytes served as an antigen in the test. The specificity of the test was revealed by absence of cross-reactivity between the antigen and sera from dogs infected with various common pathogens and specific sera against eight rickettsial species. The accuracy of the test was ascertained by isolation of the organism from reactor dogs located in and outside the United States. Histopathological examination of nine reactor dogs revealed plasmacytosis of meninges and kidneys in eight of them.

Animals↗