Biomedical subjects

D L Odor

Publications and source records attributed to D L Odor.

At least 19 recordsLinked to original sources

PubMed1995

Morphological study of changes in the baboon oviductal epithelium during the menstrual cycle.

Oviductal epithelium of the baboon, Papio cynocephalus, was studied utilizing light, scanning, and transmission electron microscopy. Results of counts made of nonciliated, ciliated, and ciliogenic cells were analyzed statistically. The percentages of nonciliated cells of the fimbria and ampulla during the early proliferative and late secretory stages of the menstrual cycle were significantly greater than those during the mid-proliferative and late proliferative-early secretory stages, due to deciliation. This paper emphasizes previously unreported apical surface morphology as viewed by scanning and transmission electron microscopy. The microvillar pattern of the fimbrial secretory cells differs from that of the ampullar and isthmic cells in that the microvilli originate from thick apical protrusions and vary greatly in length and number as related to the cycle. A ridge demarcating the apical intercellular junction is composed of rows of microvilli during the early proliferative and late secretory stages. During the early proliferative and late secretory stages an increased degree of invagination of the basal and lateral plasma membranes occurs as the height and width of the cells decreases. The general numbers and distribution of the organelles of the various types of oviductal cells agree with that described for the ampulla and isthmus by Verhage et al. [(1990) Am. J. Anat., 187:81-90]; however, fimbrial epithelium was not included in that study. Other cyclic ultrastructural changes not examined previously include variation in the number of lipid droplets and their location, and in the number and relationships of glycogen particles to other structures.

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PubMed1991

Light and electron microscopic observations on ciliated vacuoles and cysts in the oviductal and endocervical epithelia of the rabbit.

Ciliated vacuoles and intraepithelial cysts have been observed in oviductal and endocervical epithelia of rabbits. In this study, rabbits under various hormonal conditions were studied by light and transmission electron microscopy and tissue culture in an attempt to determine their distribution and origin. Ciliated vacuoles most frequently lay in the basal cytoplasm, below or beside the nucleus, and very close to the basal lamina. A few were apically located. Their average diameter was 8.8 by 5.1 microns. Cilia and microvilli projected into the vacuolar lumen. These vacuoles were located intracellularly as evidenced first by the degeneration of both their cilia and microvilli and the moderately dense matrix that often filled the vacuolar lumen, as observed by electron microscopy. Secondly, phase microscopy of the living endocervical epithelium allowed us to observe the beating of the cilia within the vacuoles, not on the surface of such cells. Thirdly, ruthenium red stained the surface glycocalyx of ciliated and secretory cells, but not that of the cilia and microvilli within the vacuoles. The intraepithelial cysts were not observed in all tissue blocks. The largest numbers were found in ovariectomized animals treated for 3 and 5 days with estradiol. More were seen in the isthmus and cervix than in the fimbria and ampulla. The cysts were located most often within the epithelium along the sides of, and at the bases of, the mucosal folds. They were lined by flattened epithelium of various combinations of secretory and ciliated cells. An unusual cell type was associated with some of the cysts and ciliated vacuoles. Its cytoplasm contained aggregates of mitochondria and vesicles whose contents varied in density. Although the genesis of the ciliated vacuoles is not certain, our results indicate that they may arise from aberrant positioning of proliferating procentrioles or from a defect in targeting or transporting the centrioles to the apical plasma membrane to serve as basal bodies. Fusion of adjacent ciliated vacuoles with lumina lined by secretory cells having deep apical invaginations appeared to contribute to the formation of cysts.

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PubMed1989

Light and electron microscopic observations on the cervical epithelium of the rabbit: II.

The endocervical epithelium of long-term ovariectomized rabbits treated for 1-10 days with 5 micrograms of estradiol benzoate every 12 hr has been studied by light and electron microscopy. In addition, morphometric data on ciliated and nonciliated cells of rabbits treated for 2, 6, and 10 days are compared to those on untreated ovariectomized, estrous, and ovulatory rabbits. The percentage of ciliated cells increases after ovariectomy to 76.3% and that of secretory cells decreases to 23.7% as compared to estrous controls. Treatment of ovariectomized rabbits with estradiol results in a gradual increase in ciliated and secretory cell area, height, and nuclear area. After 10 days of treatment, cell areas are significantly larger than those in the ovulatory or estrous controls; cell height and nuclear areas have returned to preovariectomized levels; and the percentages of ciliated and secretory cells have reached those of estrous levels. Estradiol stimulates mitotic division of secretory cells but affects ciliogenesis minimally. In ciliated cells, estradiol treatment results in a modest increase in polysomes and granular endoplasmic reticulum and in striking increases in the size of the Golgi complex and in the number of lipofuscin bodies as compared to those in the ovariectomized controls. In secretory cells, estradiol treatment brings about an increase in the numbers of polysomes, Golgi complexes, and cisternae of the granular endoplasmic reticulum, in the sizes of the nucleoli, and in the amount of euchromatin. Secretory granules appear in some cells after 2 days of estradiol stimulation and increase in number through 10 days of treatment. Perinuclear granules are more pleomorphic and heterogeneous in structure and more numerous in the 6- to 10-day-estradiol-treated than in ovulatory animals, and they may function as lysosomes degrading excess secretory product. Deep apical concavities of the secretory cells occur most often after 2 and 6 days of treatment.

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PubMed1988

Light and electron microscopic observation on the cervical epithelium of the rabbit. I.

The light and electron microscopy of the cervical epithelium of ovulatory, estrous, and long-term ovariectomized rabbits have been studied to determine what structural changes occur under different hormonal conditions. The percentage of nonciliated secretory cells is 49.6 in ovulatory, 43.6 in estrous, and 23.7 in long-term ovariectomized rabbits, and of ciliated cells is 50.2 in ovulatory, 56.2 in estrous, and 76.3 in long-term ovariectomized animals. The values for the ovulatory and estrous rabbits are significantly different at the P less than 0.05 level from those of the ovariectomized animals. In all 3 groups the general ultrastructure of the normal ciliated cells is similar. Interestingly, the Golgi complex is very prominent in all. Glycogen bodies occur frequently only in ciliated cells of ovariectomized and occasionally of estrous animals. Abnormalities in ciliation are quite common in the ovariectomized rabbits. The structure of the nonciliated secretory cells varies appreciably within and between the 3 groups. In these cells from well-developed epithelia of certain ovulatory and estrous animals, the apical cytoplasm contains secretory granules of at least three types. In addition, very irregularly shaped, dense, perinuclear granules occur, which may be another type of secretory granule or lysosomes. As compared to ciliated cells, the secretory cells have less prominent Golgi complexes, more abundant bundles of intermediate filaments, a more extensive glycocalyx on their apical surface, and more heterochromatic nuclei. In comparison to the cells of well-developed epithelia, the nonciliated cells of some other ovulatory and estrous rabbits are less well differentiated with fewer or no secretory granules and less well developed organelles. In the nonciliated cells of the long-term ovariectomized rabbits, there are no secretory or dense perinuclear granules. There is a decrease in the number of organelles that are involved in secretion, in the size of the cells, and in the amount of nuclear euchromatin.

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PubMed1985

Observations on the solitary cilium of rabbit oviductal epithelium: its motility and ultrastructure.

Solitary cilia have been observed on rabbit oviductal epithelial cells. In tissue cultures of fimbrial epithelium of 3- and 4-day-old animals observed by phase microscopy, most of these single cilia exhibited a vortical or funnel-type movement while others had the usual to-and-fro motility. Primary cilia are usually considered immotile. Transmission electron microscopy of specifically identified single cilia revealed differences between the ciliary shafts and basal bodies of the single cilia as compared to those of mature oviductal ciliated cells. The basal body of the solitary cilium often had at least two triangular, striated, basal foot processes, lacked electron-dense satellite material around its basal end, and occasionally had striated rootlets. In contrast, the cilia of mature ciliated cells had only one basal foot, exhibited much electron-dense satellite material, and lacked rootlets. Cross sections of the single cilia showed patterns of microtubules different from the usual 9 + 2 axonemal complexes of normal cilia and included 9 + 0, 10 + 2 singlets, 7 + 2 doublets, and 8 + 1 doublet and 2 singlets; one did have the usual 9 + 2 arrangement. We postulate that the presence of more than one basal foot process may be responsible for the vortical motility observed. The primary cilia are shorter than normal cilia; the longest one measured was 1.86 micron in length, 0.28 micron in width at its base, and 0.14 micron at its tip. Based on the light-microscopic, scanning-electron-microscopic and transmission-electron-microscopic observations, such solitary cilia were observed more frequently in the oviductal tissues of the 3- to 4-day postnatal rabbits grown in tissue culture and in ovariectomized and ovariectomized/progesterone-treated adult animals than in estrous, ovulatory, or ovariectomized/estradiol-treated rabbits.

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PubMed1983

Secretory cells of the oviduct of the pig-tailed monkey, Macaca nemestrina, during the menstrual cycle and after estrogen treatment.

The secretory cells of the oviductal epithelium in the pig-tailed monkey, Macaca nemestrina, have been studied by light and electron microscopy. Changes during the menstrual cycle and after ovariectomy, with and without subsequent estrogen treatment, have been documented. During the early follicular phase the epithelium is recovering from deciliation and secretory cell atrophy that occur in the late luteal phase. A few fimbrial and a moderate number of ampullar and isthmic cells contain a few electron-dense, homogeneous secretory granules in their apical cytoplasm. During the late preovulatory and early post-ovulatory periods, secretory cell structure varies considerably. Fimbrial cells typically display apical protrusions that contain no or a few small, mainly homogeneous, secretory granules. The cytoplasm is crowded with elements of the Golgi complex, with granular endoplasmic reticulum profiles often intimately associated with mitochondria, and with variable numbers of polysomes and glycogen granules. In ampullar and isthmic cells secretory granules are more abundant than in fimbrial cells, and electron-lucent vacuoles appear. The granules are of two types: 1) those having an electron-dense, homogeneous matrix, and 2) those possessing lamellar structures within moderately dense matrices. The lamellae of the second type course in parallel arrays separated by a distance of approximately 15.5 nm and exhibit a periodicity of approximately 11.3 nm. Possible transitional stages between the lamellar granules and the vacuoles containing lamellar fragments are observed. Secretion occurs by exocytosis. During the late luteal phase no fimbrial cells have secretory granules. In the ampulla many of the cells have poor development of the organelles involved in secretory activity and have few or no secretory granules. In others, a moderate number of secretory granules are present; in one animal, exocytosis is observed. In the untreated ovariectomized animal no secretory granules occur, and the organelle content is much less than in the cycling and the estrogen-treated monkeys. In ovariectomized, estradiol-treated monkeys, some areas of all three oviductal segments are well stimulated whereas others display little or no secretory activity.

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PubMed1980

Cyclic variations in the oviuductal ciliated cells during the menstrual cycle and after estrogen treatment in the pig-tailed monkey, Macaca nemestrina.

The epithelium of the oviduct of the pig-tailed monkey, Macaca nemestrina was studied 1) to determine whether quantitative changes in the number of ciliated, deciliated, reciliating and nonciliated cells occur during the menstrual cycle and under certain experimental conditions and 2) to describe the ultrastructure of the ciliated and ciliogenic cells. The mean percentage of ciliated cells decreased from 48.2 in the fimbriae and 48.3 in the ampullae in the postovulatory stage to 7.7 and 18.8, respectively in the late luteal phase; these changes are significant as determined by Duncan's multiple range test. In the early follicular phase 3.9% of the cells in the fimbriae and 11.2% in the ampullae are ciliated, and the number of ciliogenic (deciliated and reciliating) cells is the highest of any time in the cycle in both the fimbrial (6.3%) and ampullar (8.4%) epithelium. In contrast, although the percentage of ciliated cells in the isthmus varies from 44.4 in the preovulatory phase to 34.3 in the early follicular phase, the differences between the various times in the cycle are not significant. However, in the late luteal phase, the values for the fimbriae and ampullae are significantly different from that of the isthmi. Ciliated cells constitute less than 1% of both the fimbrial and ampullar epithelium 2 3/4 years after ovariectomy, but 16.7 in the isthmic tissue. In ovariectomized monkeys treated for 7 or 12 days with estradiol benzoate reciliation occurs, but to a significantly lesser extent in the fimbriae and ampullae than in the pre- or postovulatory animals; the degree of reciliation in the isthmus is not different from the values noted during the cycle. The ultrastructure of ciliated, deciliated and reciliating cells is described. Of much interest is the finding of cytoplasmic protrusions containing variable numbers of ciliary axonemal complexes. It is postulated that such internalization of ciliary micotubules may represent one way in which deciliation may be accomplished.

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PubMed1978