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D L Osheim

Publications and source records attributed to D L Osheim.

9 recordsLinked to original sources
PubMed1985

Carbofuran toxicosis in cattle: case history and analytical method.

Thirteen head of crossbred Brahman cattle were exposed to carbofuran which had been mixed with rice to control blackbirds. Three animals died within 20 minutes after eating the contaminated grain, and the other 10 exhibited clinical signs associated with carbamate toxicosis. The 4 most severely affected animals were treated with a subtherapeutic dose of atropine sulfate; they did not respond and subsequently died. The remaining 6 recovered. Samples of rumen contents from the animals that died contained carbofuran concentrations ranging from 2 to 51 mg/kg.

Animals↗
PubMed1983

Atomic absorption determination of serum copper: collaborative study.

Preliminary to conducting a collaborative study on a method for copper in serum, methods used by a selected group of laboratories were surveyed. The responding laboratories were supplied with a Youden pair of bovine serum samples and requested to use their current method for serum copper. Results of the analyses and the methods used were evaluated; hypotheses were developed in our laboratory to explain some of the interlaboratory variation. For the AAS method chosen, each of 12 collaborating laboratories analyzed one blind duplicate and 2 Youden pair of serum samples. A commercially available external control serum with a certified level of copper and a 1000 mg copper/L standard were also submitted. The method requires the serum to be diluted 1 + 1 with distilled water and the standards to be diluted with 10% glycerin to approximate the viscosity of the diluted serum. The intralaboratory coefficients of variation (CV) ranged from 2.24 to 4.40% and the interlaboratory CV ranged from 2.56% to 6.05%. The method has been adopted official first action.

Animals↗
PubMed1980

Toxaphene residues in the bovine after oral exposure.

Toxaphene was administered orally to 19 mixed-breed heifers at levels of 50, 100, or 150 mg/kg. Thirteen of 19 died with deaths in all 3 dosage levels. Liver, kidney, and brain samples were collected and analyzed for toxaphene residues by GLC. Liver residues varied logarithmically with dosage from 1.2 to 38 ppm, kidney residues were from 0.8 to 12 ppm, and brain residues were from 0.3 to 7.0 ppm. No detectable amounts of toxaphene (< 0.1 ppm) were observed in tissues collected from 2 control calves which received no toxaphene.

Administration, Oral↗
PubMedDate not supplied

Atomic absorption spectrophotometric determination of liver copper: collaborative study.

Eleven collaborating laboratories conducted replicate analyses on 4 blind duplicate pairs of bovine liver samples that either had naturally acquired copper levels or were spiked with one of 3 copper levels. A National Bureau of Standards Bovine Liver sample (SRM 1577, 193 +/- 10 mg copper/kg) and a 1000 mg copper/L standard were also submitted to the collaborators. The method requires the tissue to be digested with concentrated HNO3 at 60 degrees C, diluted to volume with water, and analyzed by atomic absorption spectrophotometry. The intralaboratory coefficients of variation (CVo) ranged from 5.6 to 19%; the interlaboratory CVx values ranged from 7.1 to 21%. The lower limit of detection was estimated to be 1 mg copper/kg tissue. The method has been adopted official first action.

Animals↗
PubMedDate not supplied

Determination of fluoride in bovine urine.

Fluoride concentration in bovine urine is determined by using a selective ion electrode. Urine is buffered and measured against known fluoride (F) standards. The method is applicable to 2 to 40 mg F/L without further dilution. Typical normal urine contains less than 10 mg F/L. Concentrations greater than 10 mg/L support a clinical diagnosis of fluorosis in cattle. Repeatability coefficient of variation (CV) ranged from 1.6 to 3.5% for F concentrations of 3.2-43 mg/L. Reproducibility CV ranged from 0.3 to 7.3% for F concentrations of 7.0-17.2 mg/L.

Animals↗