Pulse-labeled components in the replication of mitochondrial deoxyribonucleic acid.
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Animals↗
Biomedical subjects
D L Robberson
Publications and source records attributed to D L Robberson.
Covalent coupling of ribonucleic acid to agarose.
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Adenine Nucleotides↗
Replication of mitochondrial DNA in mouse L cells and their thymidine kinase - derivatives: displacement replication on a covalently-closed circular template.
Thymidine kinase(-) lines of mouse L cells incorporate thymidine exclusively into mitochondrial DNA. This fact permits assessment of labeled mitochondrial DNA components in ethidium bromide-cesium chloride gradients. Contaminating nuclear DNA is unlabeled and need not be removed. Elimination of the DNase treatment of purified mitochondria reveals that the replicative forms that exhibit displacement replication up to full genome size are covalently-closed circular molecules. Denaturation followed by brief renaturation of these larger replicating molecules produces closed-circular DNA with a deficiency of Watson-Crick turns, appearing as single-strand loops. This result suggests that displacement replication proceeds with nicking and rapid closure of the covalently-closed circular template.
Animals↗
Replication of mitochondrial DNA. Circular replicative intermediates in mouse L cells.
The frequency, composition, and structure of circular replicating forms of mitochondrial DNA, including two new forms described here, suggest a scheme for the mode of replication of this DNA.
Animals↗
A novel closed-circular mitochondrial DNA with properties of a replicating intermediate.
A fraction of the covalently closed mitochondrial DNA in mouse L cells contains a replicated heavy-strand segment that is hydrogen bonded to the circular light strand. The inserted single strand is dissociable from the circular duplex at an elevated temperature.
Animals↗