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D L Warkentin

Publications and source records attributed to D L Warkentin.

11 recordsLinked to original sources
PubMed1988

Development and performance of a fully automated method for assay of C-reactive protein in the aca discrete clinical analyzer.

A quantitative immunoassay for C-reactive protein (CRP) has been developed for use in the Du Pont aca discrete clinical analyzer. Particle-enhanced turbidimetric immunoassay (PE-TIA) technology is used. The method has a CV of less than 10% in the range 2 to 120 mg/L. Neither hemolyzed samples (Hb less than 5 g/L), icteric samples (total bilirubin less than 300 mg/L), lipemic samples (triglyceride less than 15 g/L), nor some commonly used drugs interfere. Dithioerythritol is used to eliminate interference from rheumatoid factor. Good correlation was seen when the Du Pont CRP method was compared with the Beckman ICS, Syva EMIT, TDx, and Behring methods for CRP. The normal reference interval is 0 to 9 mg/L. The method, which is fully automated, is fast, requires only a few microliters of serum, and is well suited to emergency-room requirements.

Autoanalysis↗
PubMed1985

Three techniques compared for detecting bacteriuria in symptomatic patients.

We wanted to determine whether the microscopic evaluation of urinary sediment could be replaced by either a biochemical determination (Chemstrip-9) or a colorimetric staining procedure (Bac-T-Screen), and to evaluate the feasibility of omitting from urinalyses attempts to culture urines. Cultures were considered positive when colony counts were greater than or equal to 10(3) for catheterized patients and greater than or equal to 10(4) for noncatheterized patients. The results of three separate studies on symptomatic patients showed a progressive decline in the sensitivity of the Chemstrip-9, which is a test for leukocyte esterase activity, and a difference in the sensitivity of the Bac-T-Screen between two of the studies. Neither test was consistently more sensitive or more predictive of a positive culture than was urine microscopy. By the end of the third study, we were convinced that the three methods are comparably sensitive and specific. Because 13 to 36% of positive cultures would be missed by these techniques, urine from symptomatic patients should routinely be cultured.

Bacteriuria↗