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Biomedical subjects

D Laky

Publications and source records attributed to D Laky.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Experimental investigations in prolonged myocardial ischaemia. Note II. Biology of the myocardial fiber during permanent ischaemia.

The dynamics of morphologic and biochemical lesions of the myocardial fiber during the first ten days after an experimental myocardial infarction showed an unequal evolution of the lesional picture in the ischaemic area, the intervention of lysosomal hydrolases and the reactions of inflammatory fibrocollagen substitution. Morphologic aspects in the areas neighbouring the infarction and other myocardial areas are discussed.

Animals↗
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Experimental study of myocardial bridges.

Myocardial bridges were reproduced in dogs by transfer of a fragment of the left ventricular wall above the upper portion of the anterior interventricular artery. The viability of this bridge was shown by coronarography, anatomic, histologic and electron microscopic studies, illustrating the vascular patency and the integrity of blood vessels and neighbouring myocardium in the cases postoperatively heparinized. The experimental model efficiency was also proved by ECG examinations revealing ischemic distresses induced by compression of the bridge against the underlying artery.

Animals↗
PubMedDate not supplied

[Adaptation of the myocardium to ischemia: study of suspension of the mitochondria as related to the development of the collateral circulation].

The authors developed an experimental model by means of which they established in the dog the role of short duration ischaemia (1-5 minutes) in triggering local haemodynamic changes that led to the rapid development of a collateral circulation, with retrograde filling, able to furnish subsequently blood supply to the distal segment of a main coronary artery obstructed for 20 minutes. Histological study, electron microscopy, biochemical tests and mitochondrial suspensions revealed the value of the latter method, being the most reliable test for appraising the remaining postischaemic functional potential. The data obtained may serve as a basis for understanding the role of the coronary colateral circulation in man, in coronary obstruction, and the means of developing this circulation.

Animals↗
PubMedDate not supplied

Investigations on the morphophysiological basis of revascularization of the myocardium by the Vineberg method.

A study was carried out on the dynamics of vasculogenesis and the formation of mammary-coronary anastomoses within the first seven months after implantation of the internal mammary artery in the left ventricular myocardium, according to the Vineberg method. The efficiency of these anastomoses was demonstrated 4 to 7 months later by the absence of lesions 8 to 10 days after transitory and permanent myocardial ischaemia induced by coronary obliteration. The lesions could not be prevented in the case of thrombosis of the internal mammary artery or when ischaemia was produced concomitantly with the implant or before the mammary-coronary arteriolo-arterial anastomotic network developed.

Animals↗
PubMedDate not supplied

Ultrastructural and biochemical alterations induced by propranolol treatment in experimental myocardial ischaemia.

The effect of propranolol within the first ten days after a transient experimental myocardial ischaemia was investigated. As compared to the untreated control lot, the ultrastructural and biochemical lesions appeared to be much reduced and within reversible limits. Worthy of note was the slight affection of mitochondria revealed by the level of mitochondrial enzymes, the presence of lysosomal and phagolysosomal structures and capillaries of normal aspect, and the absence of cytolysis. The favourable effect of this beta-blocking drug might be attributed to its protective action on the cellular and capillary membrane systems.

Animals↗
PubMedDate not supplied

Pathogenesis aspects of arthrosis in histopathological and electronmicroscopical studies of articular cartilage, correlated to some immunological parameters.

Twenty-five biopsies of arthrosic cartilage with radiological correspondence, arthro, sic cartilage under study for Rheumatoid Arthritis (RA) and posttrauma cartilage as control-were examined using histopathological (HE, VG, PAS-Alcian, Gömöri, Safranine O) and electronmicroscopical techniques. The arthrosic cartilage with radiological correspondence shows superficial and deep fissures, perichondrocytic gaps and modified reticulino-collagenic network at the histopathological examination. At the level of synovia-cartilage junction, we found some villous aspects of the synovia desquamating in the proximity of the affected cartilage. The investigated arthroses for RA presented some destructive fibrous modifications of the synovia similar to rheumatoid synovitis and associated with some dystrophic chondrocytic alterations. The ultrastructural affection was severe leading to cellular degeneration. The immunologically-studied arthroses for RA had seric pathologic values regarding: circulating immune complexes (CIC) (mean = 67.08 +/- 1.45 U), Ig.M(mean = 358 +/- 3.02 UI/ml) and anti collagen antibodies (mean = 409.9 +/- 0.42 U). The synovial depletion of complementary fraction C3(mean = 42.3-1 mg%) as against the normal seric level (mean = 63.07 +/- 0.49 mg%) suggests an immune synovitis. Correlation of immunomorphopathological data emphasize that arthrosis coexists with a secondary synovitis following a primitive degenerative process and allows arthroses under study for RA to be separated from other degenerative rheumatism diseases.

Antigen-Antibody Complex↗
PubMedDate not supplied

Histopathological and ultrastructural modifications of the arthrosis articular cartilage.

Thirty samples of articular cartilage taken during the operation from patients with incipient arthrosis, arthrosis with radiological modifications and arthrosis under study for Rheumatoid Arthritis (RA) were investigated using histopathological (HE, VG, PAS-Alcian, Gömöri, Safranine O) and electronmicroscopic techniques. The control material was made of posttraumatic cartilage (Moore prosthesis). Histopathologically, the incipient arthrosis cartilage had superficial exfoliations associated with reduced saframinophilic tinctorial perichondrocytic activity. The arthrosic cartilage with typical radiological modifications was individualized at the synovia-cartilage junction by villous aspects of the synovia associated with perichondrocytic gaps, reduction of safraninophilia and modifications of reticuline-collagenic network. The arthrosic cartilage under study for RA revealed destructive fibrous modifications of the synovia and severe affection of the articular cartilage at synovia-cartilage junction. Electronmicroscopically, the ultrastructural affection was minimum in the incipient arthrosis cartilage developing to chondrocytic degeneration in arthrosis with radiological correspondent. Both histopathological and ultrastructural data emphasize the fact that arthrosis is associated with synovitis following a primitive degenerative process similar to rheumathoid synovitis in arthrosis under study for RA.

Biomarkers↗
PubMedDate not supplied

Histoenzymological and ultrastructural studies of the macrophage-type pulmonary cell modifications under different chronic stasis conditions.

The optical histoenzymological and electronomicroscopical investigations were carried out en 90 pulmonary biopsies taken from patients under different chronic stasis conditions. To investigate macrophage-type cells we studied the reactions of acid phosphatase and non-specific esterase like enzyme markers, indicating the metabolic state activation degree of this cellular compartment. The ultrastructural examinations revealed the existence of two types of activated pulmonary macrophages. The former is the alveolar macrophage with a prevailing phagolysosome apparatus that pleads for phagocytic function and the latter is the interstitial macrophage with subcellular aspect corresponding to the synthesis and secretion function. The presence of these activated alveolar and interstitial macrophages, as well as their extremely increased number, plead for their direct participation in the appearance and aggravation of the morphophysiopathological disorders in the chronic stasis lungs.

Enzyme Activation↗
PubMedDate not supplied

Interference of some enzymatic modulators in the hepatic aggression induced by xenobiotics.

The aim of the paper was to compare the erythrocyte serum and hepatic chomogenate antioxidative factors in order to assess their involvement in the detoxification events. The catalase and superoxiddismutase levels, important factors of the cellular defence, were sensitivity modulated in an acute experiment on Wistar rats. Carbofuran was administered in a non-lethal dose (7 mg/b.w.) single or in the presence of certain antioxidative agents (Vitamin E, Caffeine, Aspirin) EDTA and Cysteine for their role in protecting membranes against oxidative damage. The erythrocyte parameters (SOD, Catalase) were well related to seric factors, especially ceruloplasmin level, with varied magnitudes. GGT a marker of hepatotoxicity and G1-DH, a mitochondrial marker, were in a good correlation with erythrocyte factors. The changes seem to modulate a transmembranary disturbance process, as in hepatocyte pictures.

Animals↗
PubMedDate not supplied

Histopathological and histoenzimological investigations of the rheumatoid articular cartilage.

Twenty seven biopsies of articular cartilage taken intraoperatively from patients with Rheumatoid arthritis (RA) and from control patients with traumas were examined using histopathological techniques (HE, VG, PAS-Alcian, Gömöri, Safranine 0) and histoenzymological techniques (Acid phosphatase-lysomal marker, Chondroitinsulphatase, Peroxidase). Histopathologically, the rheumatoid articular cartilage appears with superficial and deep cartilaginous fissures, frequent perichondrocytic gaps associated with modification of the tinctorial activity. At the pannus synovia-cartilage junction we found invasive and destructive synovial inflammatory infiltrates penetrating and eroding the cartilage. Histoenzymologically, the rheumatoid chondrocytes have a high lysosomal potential (phosphatasic, chondroitinsulphatasic) and highly oxidative potential (peroxidasic) specific for lesion modifications.

Acid Phosphatase↗
PubMedDate not supplied

Extensive cutaneous lymphoma.

The case of a 35-years-old patient with a progressive and densely developed subcutaneous tumour comprising the whole right cervical and subcutaneous region is presented. The tumour was painless of pale brown and light red colour, with no adherence on the epidermis and subjacent muscles. The patient has no pathological symptoms. After the histopathological examination we found the dermal infiltration with small, round or oval hyperchromatic cells and the histoenzymological investigation revealed intense positive markers CLA and MB2 pleading for their leukocytic nature while L26 reaction attested the B-cell nature of these lymphocytes and the diagnosis of polymorphous centroblastic malignant lymphoma (K type Working Classification).

Adult↗
PubMedDate not supplied

Aspects of ankylosing spondylarthritis immunopathogenesis correlated with some immunoseric- and synovial parameters in the investigation of histopathological and electronmicroscopic alterations of the articular cartilage correlated with some immunoseric and synovial parameters.

Eighteen biopsies of articular cartilage taken intraoperatory from patients with Ankylosing Spondylarthritis (AS) and from others with traumatisms (controls) were investigated using histopathological (HE, VG, PAS-Alcian, Gömöri, Safranin 0), electronmicroscopic and histoenzymamologic techniques. Histopathologically, the synovitis in AS is characterized by abundant synovia lymphoplasmocytic infiltrates associated with aspects of vascular hyperplasia and fibrosis. At the pannus synovia-cartilage junction we found the invasive synovia lymphoplasmocytic infiltrates. The proteoglycan (PG) depletion is confirmed histopathologically by diminishing the Safranin 0 staining, then ultrastructurally by the existence of collagen revealing areas, whereas biochemically, by the presence of glycosaminoglycans (GAG) in serum and synovial fluid (SF). The morphological data were related to some immunological parameters involved in pathogenesis. In this way, we found pathological values of the immune circulating complexes (ICC) (serum, mean = 73.5 U; SF mean = 81.80 U) and of anti Collagen II antibodies (serum mean = 410 U; SF mean = 436 U). The reactive protein C acting in the phase (CRP) showed high pathological values both in serum (mean = 5.01 mg%) and in SF (mean = 3.6 mg%) of the patients with AS, emphasizing the inflammatory characteristics of the rheumatic disease. The presence of ICC, anticollagen II antibodies and GAS as well in synovia suggests that the inflammatory articulation in AS is a local potential antigen of collagen and proteoglycan nature.

Cartilage, Articular↗
PubMedDate not supplied

Aspects of immunopathogenesis of rheumatoid arthritis correlated with some immunological parameters in histopathological and electronmicroscopical investigations of the articular cartilage.

The histopathological (H. E., V. G., PAS-Alcian, Safranine 0, Gömöri) and electron-microscopical investigations were carried out on twenty samples of articular cartilage taken during operations from patients with Rheumatoid Arthritis (R. A.) and from others with traumatism, as controls. Histopathologically, the rheumatoid synovial membrane is characterized by synovitis with abundant perivascular lymphoplasmocytic infiltrates. At the pannus synovia-cartilage junction we found the invasive and destructive inflammatory infiltrates penetrating and eroding the cartilage. The histopathological characteristics of the rheumatoid articular cartilage lie in alteration of tinctorial activity, affection of reticuline collagen network and the presence of superficial and deep cartilaginous fissures. The histopathological alterations were confirmed ultrastructurally. Immunologically we found pathological serum values regarding the immune circulating complexes (I. C. C.) (mean = 104 +/- 1.04 U), anticollagen II antibodies (mean = 538 +/- 5 U), reactive Protein C (mean = 16.75 +/- 1.95 mg%) and orosomucoid (mean = 151.1 +/- 4.91 mg%), in seropositive R. A. The corroboration of histopathological, electronmicroscopical and immunological data show the inflammatory and autoimmune feature of this rheumatic disease.

Arthritis, Rheumatoid↗