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Biomedical subjects

D Lange

Publications and source records attributed to D Lange.

At least 19 recordsLinked to original sources
PubMed1990

[Elements of the early diagnosis and staging of prostatic cancer. Functional results of radical surgery. Apropos of 50 complete prostatectomies].

The authors report a series of 50 radical prostatectomies and analyse and discuss the elements of: early diagnosis of adenocarcinoma: 63% of patients did not have a strictly normal digital rectal examination, 70% had abnormal ultrasonography, 73% has a PSA assay greater than 10. Suspicious signs were absent in 17% of cases, but present in 83% of cases (one sign), 63% of cases (two signs) and 40% of cases (three signs). Ultrasound guided biopsy of suspicious zones (on rectal examination or on ultrasonography) and in adjacent zones by dividing the prostate into quadrants has an increasing diagnostic yield (77% of the last thirty cases, 100% of the last fifteen cases). Staging frequently underestimates the exact volume of the tumour and the state of the prostatic capsule. MRI using a high power magnetic field apparatus seems to improve the accuracy of preoperative staging. Operative results were excellent in terms of mortality (nil), morbidity (6% of cases) and functional results for continence (recovered within one month) and sexual activity (satisfactory in 73% of cases) when Walsh's technique was able to be applied.

Adenocarcinoma↗
PubMed1990

[A clinical trial of pefloxacin (Abaktal) in the treatment of acute pyelonephritis].

The therapeutic effect of Pefloxacin in the treatment of acute pyelonephritis was examined in a randomized clinical trial. The patients in the control group have been treated by gentamycin. With a success rate of 80% in the treatment of acute pyelonephritis caused by various bacteria (E. coli, Pseudomonas, Klebsiella, Staphylococcus aureus, Proteus mirab.), a side frequency of side effects and a good compliance Pefloxacin is recommended as an effective antimicrobiell drug.

Acute Disease↗
PubMed1987

Localized injections of botulinum toxin for the treatment of focal dystonia and hemifacial spasm.

Medical treatment of dystonia usually results in an incomplete response and is frequently unsuccessful. Peripheral surgical therapy is available for some focal dystonias, but may only offer temporary relief and may have unacceptable complications. We have used local injections of botulinum toxin into the appropriate muscles for treatment of disabling focal or segmental dystonia in 93 patients with torticollis, blepharospasm, oromandibular dystonia (OMD), limb dystonia, lingual dystonia, and dystonia adductor dysphonia, in addition to four patients with hemifacial spasm. Significant relief of motor symptoms was seen in 69% of the patients with blepharospasm and 64% of patients with torticollis; 74% of the latter group with pain experience relief. Relief of symptoms was noted in most patients with OMD and limb dystonia, and all with lingual dystonia, dystonic adductor spastic dysphonia, and those with hemifacial spasm. Benefit averaged 2 1/2-3 months initially; however some patients experienced longer relief with subsequent injections. Adverse effects were transient, although 2 patients developed antibodies against the toxin, and we documented evidence for distant effects in others. This approach of chemically weakening contracting muscles in focal dystonia offers many advantages over pharmacotherapy and surgical therapy. Additional experience is needed to explore the proper doses, and potential for long term adverse effects.

Adult↗
PubMed1982

Restriction patterns of adult chicken globin genes at early and late stages of embryonic development.

Globin mRNA isolated from anemic chicken was transcribed into cDNA and integrated into the Pst I cleavage site of plasmid pBR 322. After cloning in E. coli strain HB 101 and colony hybridization with 125I-labelled globin mRNA the plasmids of individual clones were characterized by hybrid arrested cell-free translation. Thus we could isolate clones containing alpha or beta globin chain nucleotide sequences. DNA was isolated from chicken blastoderms incubated for 18-20 h and from 11 d chicken embryos. A comparison of the restriction maps of the DNA from the two developmental stages with labelled nick translated plasmids and labelled cDNA did not indicate any globin gene rearrangements between these two stages of embryonic development. We conclude, that the adult chicken globin genes show a constant genomic organization during embryonic development. However, the restriction patterns of the globin gene family of the chicken strain investigated revealed some differences after 2 generations of propagation.

Animals↗
PubMed1979

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization.

The genes for ribosomal RNA of chicken are present in about 200--240 copies per haploid genome. The ribosomal genes were separated from the bulk of DNA by different methods. In CsCl-actinomycin D gradients the GC-rich ribosomal DNA is shifted to the lower density side of the bulk of DNA. After hybridization with iodinated ribosomal RNA under conditions where R-loops are formed ribosomal DNA has an increased density. The R-loops can be separated from the bulk of DNA in Cs2-SO4 gradients.

Animals↗
PubMed1978

Radiation dose and labeling of hippuran.

Renal morphology and renal function were studied with iodine-123-hippurate in 98 patients with various renal diseases. Sequential scintigraphy with iodine-123-hippurate demonstrates much better renal blood flow, morphology and function than iodine-131-hippurate. A method of labeling hippurate in a short time (2 h) with high efficiency (95--100%) is described. Radiation exposure to various organs was calculated and significant differences in radiation exposure to the thyroid are demonstrated.

Hippurates↗
PubMed1977

[Estimation of regional cerebral blood flow with the gamma-camera (author's transl)].

Using a computerized gamma camera regional cerebral blood flow and regional cerebral blood transit time are measured after injection of Xenon 133 and Technetium 99m respectively into the internal carotid artery. Equipment, technical procedures and methodology are described. Typical cases are presented to describe the usefullness of this procedure.

Brain Edema↗
PubMed1976

Molecular weights, poly(A)--content, and partial separation of chick globin mRNAs.

Poly(A)-containing 9S RNA from chick reticulocytes was electrophoresed on formamide-polyacrylamide gels. The molecular weight was determined to be 211 000 +/- 10 000 daltons. The RNA was separated into three different fractions with respect to molecular weight. These RNAs were translated in a wheat germ cell-free system. The lower molecular weight RNA directed up to 95% alpha-chain synthesis, compared to 60% for the higher molecular weight RNA. This was accompanied by a relative increase for beta-chain synthesis with increasing molecular weight. It could also be shown by hybridization with labelled poly(U) that the average poly(A) length decreased from about 83 nucleotides for fraction I to 36 nucleotides for fraction III. Our results suggest that fractionation of avian 9 S globin mRNA by electrophoresis on formamide-polyacrylamide gels is dependent upon two parameters, namely differences in the lengths of the non-poly(A)-containing portion of the alpha and beta mRNAs and differences in the poly(A) lengths.

Animals↗
PubMed1976

[Ca and P metabolism in the skeletal system of the dairy cow as shown in histometric follow-up studies on the substantia spongiosa of the tuber coxae].

1. Histometric follow-up checks of the Substantia spongiosa of Tuber coxae in dairy cows have shown the trabecular percentage of the skeleton to decline continuously from the onset of lactation and to reach its lowest point in the last third of the lactation period. This was followed by some rise up to the next lactation, but the original values were not restored. 2. The percentage of spongiosa trabeculae, consequently, drops along with growing age, and so does the absolute trabecular surface. 3. No unambiguous dependence of trabecular surface on milk yield was established. 4. The results obtained from the above checks are discussed with reference to the physiological dynamics of the bone system as a mobile store of minerals. Some of the aspects mentioned are of greater relevance to practice.

Animals↗