An investigation into the causes of massive splenomegaly at the University Teaching Hospital, Lusaka, Zambia.
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Adolescent↗
Biomedical subjects
D Levitt
Publications and source records attributed to D Levitt.
A study of the diurnal variation of plasma cortisol in normal subjects and hospitalised patients in Zambia.
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Adolescent↗
Control of chondrogenesis in limb-bud cell cultures by bromodeoxyuridine.
Initial exposure of cultured limb-bud cells (stage 23-24) to 5-bromo-2'-deoxyuridine (BrdU) irreversibly inhibits differentiation to cartilage under three different culture conditions. The inhibition of chondroitin sulfate synthesis is partially reversed by D-xylose in limb-bud cells after treatment with BrdU. The activities of four enzymes involved in chondroitin sulfate production were reduced in BrdU-treated cultures, but the magnitude of decrease was far less than the decrease in glycosaminoglycan synthesis. The slight increase in the turnover rate of sulfated glycosaminoglycans in BrdU-treated mesenchyme was not sufficient to account for the marked decrease in chondroitin sulfate content. The results suggest that BrdU treatment interferes with normal synthesis of chondroitin sulfate core protein in cultured limb-bud cells, but does not greatly diminish enzyme activities or UDP-sugar levels necessary for production of polysaccharide chains.
Acetates↗
The irreversible inhibition of differentiation of limb-bud mesenchyme by bromodeoxyuridine.
Growing freshly dissociated chick-limb bud cells (stage 24) over agar for 48 hr permits differentiation into cartilage upon monolayer culture even when initial plating and subculture densities are well below confluency. Addition of 5-bromodeoxyuridine (BrdU) during the initial (48-hr) period over agar irreversibly inhibits chondrogenic differentiation, as characterized by morphology, metachromasia, and sulfate incorporation into acid mucopolysaccharide. Simultaneous, but not subsequent, addition of excess thymidine will prevent the effect of the analogue. Collagen synthesis is not depressed in BrdU-treated cells. Radioautographic studies demonstrate the specific localization of BrdU in the nucleus. Treatment of trichloroacetic acid-precipitable material containing tritiated bromodeoxyuridine with deoxyribonuclease solubilizes 90% of the radioactivity. The loss of the analogue from this precipitable material upon prolonged culture of limb-bud cells is more rapid than can be expected from cell division alone. 5-Bromodeoxyuridine may affect a fraction of DNA involved in stabilization of the differentiated cell phenotype.
Animals↗
Chronic peritoneal dialysis in the management of chronic renal failure.
Three patients with indwelling Tenckhoff catheters were maintained on peritoneal dialysis using an automated reverse-osmosis dialysis machine. All showed striking improvement. Peritonitis developed in two and necessitated catheter replacement in one. Strict aseptic technique in the dialysis procedure is emphasised, prevention of peritonitis being the key to success. Long-term peritoneal dialysis offers three advantages: it is a practical alternative to haemodialysis in selected cases, especially where trained personnel are few; it is simpler and cheaper.
Adult↗
Serum immunoglobulin levels in the Zambian African.
Serum immunoglobulin concentrations were determined in 121 Zambian in-patients suffering from different acute and chronic disorders. The immunoglobulin response was similar to that observed in other patient populations.
Humans↗