Biomedical subjects

D M Chapman

Publications and source records attributed to D M Chapman.

4 recordsLinked to original sources

PubMed1978

The role of IgA in the prevention of bacterial colonization of the jejunum in the vagotomized subject.

The efficacy of three mechanisms of defence against jejunal bacterial colonization (i.e. gastric acid, vagal and pyloric integrity and intestinal IgA) has been investigated. Only in subjects with disturbance of all three mechanisms did significant jejunal colonization occur, and this was invariably associated with severe diarrhoea. Clearly normal intestinal IgA is of critical importance in the prevention of jejunal bacterial colonization in the vagotomized subject.

Diarrhea↗

PubMed1976

Association of HLA-A9 and HLA-B5 with Buerger's disease.

Eighteen patients who satisfied stringent criteria for the diagnosis of Buerger's disease, healthy controls, and patients with atherosclerosis were tested for various HLA antigens. The incidence of HLA-A9 and HLA-B5 was significantly greater among those with Buerger's disease. This finding supports the concept that Buerger's disease is a distinct clinicopathological condition.

Adult↗

PubMed1976

IgA deficiency and severe post-vagotomy diarrhoea.

Between January, 1969, and December, 1973, 2058 truncal vagotomies were performed in the Merseyside Regional Health Authority area. 14 of these patients subsequently developed severe post-vagotomy diarrhoea and were extensively investigated. 6 were found to have IgA deficiency. It is suggested that antecedent IgA deficiency may account for the varied reported incidence of severe post-vagotomy diarrhoea and that preoperative screening could reduce the incidence of this complication.

Adult↗

PubMed1975

Dichromatism of bromphenol blue, with an improvement in the mercuric bromphenol blue technic for protein.

Staining of protein in sections using the mercuric bromphenol blue technic is improved by staining with 1% HgCl2 and 0.05% bromphenol blue in 2% aqueous acetic acid for 15 min at room temperature. Rinse slides 20 min in 2 changes of 0.5% aqueous acetic acid. Blot and give 2 fast changes in absolute ethanol with agitation before transferring to xylene. Transfer slide to 0.5% n-butylamine in xylene for a few seconds until the section is blue, then, after 2 changes of xylene, mount in DPX. Spectrophotometric analysis of this blue dye at different concentrations and with or without heparin showed that the reddish hues are due to dichromatism and not metachromasia.

Animals↗