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Biomedical subjects

D M Goldfarb

Publications and source records attributed to D M Goldfarb.

14 recordsLinked to original sources
PubMed1980

Plasmid pEA566 from Erwinia aroideae.

A new plasmid designated pEA566 was isolated from Erwinia aroideae. The molecular weight of the plasmid, as determined by neutral and alkaline sucrose gradient centrifugation, electron microscopy, and agarose gel electrophoresis, was 6.6 X 10(6). The plasmid replicated under relaxed control, had three cleavage sites for KpnI restriction endonuclease, and no sites for EcoRI, BamHI, SalI, PstI, and HindIII.

Erwinia↗
PubMed1978

Protection of foreign DNA against host-controlled restriction in bacterial cells. I. protection of F' plasmid DNA by preinfection with bacteriophages T3 or T7.

Foreign F'lac plasmid DNA which is introduced into potentially restricting E. coli recipient cells can be protected from restriction by preinfecting the recipient cells with UV-inactivated T3 or T7 bacteriophages which express the ocr gene function. The recipient cells survive and are able to replicate themselves as well as the newly acquired plasmid.

Coliphages↗
PubMed1978

F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K-12 HfrC.

Three related F'arg+ plasmids isolated by Guyer and Clark were used to analyze some properties of strain MG751, a recipient derivative of an HfrC mutant (MG7) carrying a previously described pleiotropic mutation in the integrated F plasmid. Strains MG7 and MG751 both failed to produce recombination-stimulating factor, were sensitive to monofunctional alkylating agents and UV irradiation, and were temperature sensitive for growth and DNA synthesis. It was shown that these phenotypes are controlled by F plasmids genes (designated rsf, prt, and rep) that can be separated by deletion mutations occurring on the F plasmid.

Chromosome Mapping↗