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Biomedical subjects

D M Ivnitskiĭ

Publications and source records attributed to D M Ivnitskiĭ.

6 recordsLinked to original sources
PubMed1991

[An electrochemical method of determining peroxidase in saliva].

Electrochemical method has been developed for measuring human salivary peroxidase. Salivary peroxidase levels were measured in donors in various hours of the day, in girls in various periods of the cycle, and in myocardial infarction patients. The results may be used to develop rapid methods for assessment of ovarian functional activity and for prediction of myocardial infarction outcome. Electrochemical analysis productivity is 360 samples per hour, the minimal peroxidase concentration detectable is 5.10(-12) M.

Adult↗
PubMed1991

[A highly sensitive immunoenzyme analysis using electrochemical detection].

The authors have developed a method for improving the sensitivity of electrochemical enzyme immunoassay, based on binding the enzymatic reaction volatile component to a complexone, followed by destruction of the complex in a flow-type amperometric detector. The lowest limit of the measured levels of blood serum antibodies and antigens is 10(-11)-10(-12) M. The method was tried in enzyme immunoassay of the blood serum IgE in patients with asthma and chronic obstructive bronchitis. Reduction of IgE level after therapy was found to be a regular feature; the authors claim that it may be considered as one of the criteria indicating the treatment efficacy.

Adult↗
PubMed1987

[Immunochemical study of modified forms of L-asparaginase].

Certain catalytic and immunological properties of L-asparaginase modified by polyglucin were studied. It was shown that the modified forms of L-asparaginase maintained high catalytic activity (Km 0.80.10(-5)-1.89.10(-5) M) and at the same time appeared to be more resistant to inactivation under the effect of antibodies.

Animals↗
PubMedDate not supplied

[Determining the peroxidase level using tubular coal electrodes in flow-type systems].

An amperometric transducer with tubular coal electrodes has been worked out to determine the microquantity of peroxidase. The hydrodynamic parameters of the transducer were studied as were the relationship of the output signal to the concentration of the electroactive substance, the length of the electrode, the speed of the background electrolyte movement and the diffusion coefficient. The line section of the relationship between the strength of current and the peroxidase concentration is observed within the interval of the enzymic concentration 1-32 ng/ml with the exposure time of the enzymic reaction of 5-10 min.

Coal↗
PubMedDate not supplied

[Amperometric sensor for recording the results of immunoenzyme analysis].

A technique for quantitative determining horseradish peroxidase was developed using an amperometric sensor that makes it possible to register up to 50 pM of the enzyme in the reaction of the peroxidase oxidation of iodide ions by hydrogen peroxide. The sensor, composed of combined carbon and Ag/AgCl electrodes, is intended for enzyme immunoassay and may be used for registering the results in the very wells of the polystyrene plates widely used in clinics and laboratories. The dependences of the initial rate of the enzymatic reaction on the substrate concentration and pH of the medium were determined, on the basis of which the conditions for the optimal operation of the sensor were found. The amperometric and spectrophotometric methods of registration on a model system for determining antibodies against X-virus of potato were compared in order to show a possibility of the sensor applying to enzyme immunoassay.

Electrochemistry↗