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Biomedical subjects

D M Pugh

Publications and source records attributed to D M Pugh.

39 records · Page 3Linked to original sources

An in vitro demonstration of oestrogenicity with potential for exploitation as a quantitative assay for oestrogenic potency.

The pre-implantation embryo of the mouse undergoes a histochemically detectable change in the properties of its trophoblastic cell-surface coat in the immediate pre-implantation period. This change is oestrogen-dependent in vivo and can be induced in vitro in a concentration-dependent manner by oestradiol-17 beta. There is evidence that this coat change is of functional importance in the process of implantation, and its demonstration is of potential value as the basis of an in vitro assay of oestrogenicity.

Animals↗

Plasma and buffy coat vitamin C concentrations in the dog.

In dogs, the mean plasma vitamin C concentration was found to be 0.88 mg/100 ml plasma while the mean buffy coat concentration of simultaneous blood aliquots was 42.98 mg/10(8) cells. A significant day-to-day variation was recorded for both parameters.

Animals↗