Biomedical subjects

D M Rocke

Publications and source records attributed to D M Rocke.

5 recordsLinked to original sources

PubMed1995

Permanent heterotopic ossification at the injection site after diphtheria-tetanus-pertussis immunizations in children who have fibrodysplasia ossificans progressiva.

In patients who have fibrodysplasia ossificans progressiva, routine childhood diphtheria-tetanus-pertussis immunizations administered by intramuscular injection caused a significant risk of permanent heterotopic ossification at the site of injection (p < 10(-8)), whereas measles-mumps-rubella immunizations administered by subcutaneous injection posed no significant risk. Intramuscular injections should be avoided, if possible, once a diagnosis of fibrodysplasia ossificans progressiva has been established.

Adolescent↗

PubMed1994

Extension of the four-parameter logistic model for ELISA to multianalyte analysis.

The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of immunoassay that use the four-parameter logistic curve.

Cross Reactions↗

PubMed1991

Robustness and balance in the mixed model.

When balanced data are analyzed by a robust method, different weights are applied to data points depending on their apparent status as outliers. This results in the effective unbalancing of the data. Concepts developed for the analysis of unbalanced data can help to suggest robust methods of analysis for balanced data.

Analysis of Variance↗

PubMed1990

Statistical design of ELISA protocols.

This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.

Enzyme-Linked Immunosorbent Assay↗

PubMed1984

On testing for bioequivalence.

A number of methods have been proposed for statistical analysis of bioequivalence trials. A new method is proposed and compared to three previously suggested techniques. This comparison clarifies the relationship among the four methods and yields practical suggestions regarding statistical tests.

Biological Availability↗