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Biomedical subjects

D M Unwin

Publications and source records attributed to D M Unwin.

5 recordsLinked to original sources

Automatic nitrous oxide synchronization of mitotic human cell cultures.

Large numbers of human cells can be reversibly arrested in mitosis by high-pressure nitrous oxide. The optimum schedule for this arrest requires that the high-pressure block be started around midnight to provide a mitotic population that can be released the next morning to progress in synchrony through the next cell cycle. We describe a simple and safe device which can be set up at the end of the day and automatically exposes the cells to high-pressure nitrous oxide overnight.

Cells, Cultured↗

An automatic contact-counter for recording object play.

The paper describes a portable electronic device which automatically records a measure of object play behaviour, by counting the number of contacts made with a play manipulandum. The device is designed for recording a measure of object play in domestic cats, but is readily adaptable for use with other species.

Animals↗