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Biomedical subjects

D M Vasudevan

Publications and source records attributed to D M Vasudevan.

At least 55 records · Page 3Linked to original sources
PubMed1985

Detection of herpes simplex virus type-2 antigen(s) in biopsies from carcinoma of the uterine cervix.

Indirect immunofluorescence technique was employed to detect herpes simplex virus type-2 (HSV-2) antigens in tumor biopsies from 215 patients with carcinoma of the uterine cervix. A total of 169 samples (79%) revealed brilliant nuclear fluorescence. Inflammatory cells infiltrating the tumor mass were positive to 60 of the 215 patients (28%). Samples showed no significant variation in the degree of fluorescence or proportion of cells binding HSV antibody with advancement in the clinical stage of the disease. Fluorescence was totally abolished when incubated with HSV-2 antiserum absorbed with a specific homologous virus. Among controls, there was fluorescence in 27% of cervical scrapings from normal women and 34% (42/124) among patients with gynecological disorders other than cervical malignancy. In cervical dysplasia 23 out of 40 patients (58%) expressed herpes virus-associated antigens. There was membrane fluorescence in live malignant cell preparations in 3 of 28 patients (11%). Normal cervix tissue from hysterectomy specimens and breast cancer cells were negative for herpes simplex virus-related antigens. Pre-immune serum and PBS showed nonspecific fluorescence in 25% and 23% of sera, respectively.

Antigens, Viral↗
PubMed1984

Leucocyte adherence inhibition assay (LAI) in cancer of the oral cavity.

Antitumor immunity to oral cancer was assessed in 46 patients suffering from squamous cell carcinoma of the oral cavity using a leucocyte adherence inhibition assay (LAI). The response shown by the leucocytes of oral cancer patients to the oral cancer antigen was compared to that shown by the leucocytes from 10 normal age- and sex-matched healthy controls and 43 patients suffering from other types of cancers. Seventy-six percent of the oral cancer patients showed a high degree of leucocyte adherence inhibition in the presence of the antigen. The specificity of this test was further assessed using combinations of the leucocytes from oral cancer patients with extracts from seven cancers occurring at various other sites and extracts of normal oral tissue. The test was highly specific and the leucocytes of oral cancer patients showed significant inhibition only in the presence of oral cancer extract. The inhibition was between 0 and 30% with most other cancer extracts except in the case of extract from cancer of the cervix, where 4/10 patients showed above 30% inhibition. Specific blocking of the LAI response was observed on addition of sera from oral cancer patients to leucocyte-antigen mixtures from oral cancer patients. This effect was not observed on addition of these sera to specific leucocyte-antigen mixtures from other cancer patients. These observations point towards the usefulness of this test in monitoring antitumor immunity in oral cancer patients.

Antigens, Neoplasm↗
PubMed1983

Quantitation of Fc receptor-bearing T-lymphocytes (TG and TM) in oral cancer.

The lymphocytes from the peripheral blood of 30 untreated patients with oral cancer and 15 apparently healthy normal controls were studied for quantitation of T-cell subsets. When compared to age-matched controls, the patients as a group showed a significant increase in the proportion and number of TG cells (IgG Fc receptor-bearing T-cells) and a significant reduction in the TM cells (IgM Fc-bearing T-cells). The levels of total lymphocytes, T-cells, and B-cells remained in the control range. The discrepancy in the TG subset was evident in the early stages of the disease, while in TM cells, it was evident only in the advanced stages.

Adult↗
PubMed1981

Renal involvement in leprosy.

Renal involvement in 13 non-lepromatous and 17 lepromatous leprosy patients were assessed by routine urinalysis, detailed biochemical analysis of blood and urine and by renal histopathological studies and compared with 10 normal healthy controls. The presence of RBC and pus cells were detected in the urinary deposit of only one lepromatous leprosy patient in reactional phase. A reversal of albumin/globulin ratio was observed in 17.6% of non-lepromatous and 15.3% of lepromatous patients. 24 hours urinary excretion of sodium, potassium, chloride and aminonitrogen of the patients were within the normal range. Forth seven percent of the non-lepromatous and 46% of the lepromatous patients had proteinuria. Even though the mean serum creatinine values of the patients showed no difference from that of the normals, the creatinine clearance was low in 82.3% of the non-lepromatous and in all of the lepromatous patients. Serum phosphorus, serum uric acid, urinary phosphate excretion and the renal tubular reabsorption of phosphorus of the patients were normal. Twenty one percutaneous renal biopsy specimens showed nonspecific pathological changes such as nephritis of various varieties in 71.4% of the specimens. Among the lepromatous group renal involvement was observed in 5 out of 9 cases (55.6%) and in the non-lepromatous group 10 out of 12 cases (83.3%). No acid fast bacilli, amyloid and granuloma were seen in any of the renal tissues studied. There was no definite correlation between the type of renal pathology and biochemical changes. None of the patient showed any clinical evidence of renal involvement.

Adolescent↗
PubMed1980

Liver in leprosy--I. Functional changes.

Liver function tests were carried out in 79 leprosy patients, out of which 28 patients were being treated and 42 patients were not receiving any treatment. The rest 9 patients were clinically quiescent. In the untreated group there were 28 tuberculoid and 14 lepromatus cases. The 28 patients receiving treatment were composed of equal number of lepromatus and tuberculoid groups. The liver function tests of 10 of the untreated tuberculoid patients were repeated after six months of specific therapy with D.D.S. The values of liver function tests of these patients were compared with that of 20 normal adults. The most important abnormality observed in the patients were a reversal of albumin/globulins ratio which was more common in the lepromatus group than in the tuberculoid group. Treatment with D.D.S. has resulted in the reduction of the serum globulin. There were no changes in transaminases and alkaline phosphatase activities in the untreated patients. But however a rise in the above enzymes were observed in patients on treatment. The mean serum cholesterol values of the patients were lower than that of the controls.

Alkaline Phosphatase↗