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Biomedical subjects

D Málková

Publications and source records attributed to D Málková.

11 recordsLinked to original sources
PubMed1978

Arbovirus isolations from mosquitoes in South Slovakia.

In the years 1973 and 1975 mosquitoes and some other Diptera (Tabanidae, Simuliidae, Hippoboscidae) were tested for virus. 13,924 mosquitoes, 75 horseflies and 60 blackflies were processed in 1973. Five strains of Tahyna virus were isolated from mosquito species Aedes vexans. 3,378 mosquitoes and 12 sheep keds were tested for virus in 1975. Twelve strains of Calovo virus were isolated from Anopheles maculipennis and one strain of Tahyna virus was obtained from Aedes vexans mosquitoes.

Aedes↗
PubMed1977

Experimental infection of pheasants with Lednice (Yaba 1) virus.

Young pheasants (24-hour- and 7-day-old) are capable of producing viraemia and antibodies after experimental infection with Lednice virus even after low doses of virus (0.7-0.9 log mouse LD50/g). Viraemia lasts approximately 3 days, but the titres of virus in the blood are low. The possible role of young pheasants in the circulation of virus in nature is discussed.

Animals↗
PubMed1976

Influence of temperatures corresponding to those of the host animals on Tahyna virus.

The influence of temperature of 36.5 and 39.2 degrees C on uncloned low passage Tahyna virus in chick embryo cell cultures was studied in the course of 10 passages. At a high multiplicity of infection, the virus titres showed a considerable variability, especially at 39.2 degrees C. The cytopathic effect (CPE) apparent at both temperatures started later and was of lower intensity at 39.2 degrees C than at 36.5 degrees C. The temperature of 39.2 degrees C intensified the thermostable character of the virus. Changes in plaque size and virulence were connected mainly with the cultivation substrate of the virus.

Animals↗
PubMed1976

Influence of temperature corresponding to that of the vector on Tahyna virus.

The behaviour of uncloned, low-passage Tahyna virus in an Aedes albopictus (AA) cell line at 28 and 20 degrees C was studied in the course of 10 passages. The virus multiplied at both temperatures without any apparent effect on the host cell. At 28 and 20 degrees C reduction of plaque size, decrease of peripheral virulence and weakening of thermostability were observed. Differences between both temperatures were only in the intensity of these changes.

Aedes↗
PubMed1976

Cultivation of Lednice (Yaba 1) virus in goose, duck, and chick embryo cells.

Lednice (Yaba 1) virus was found to multiply in primary cultures of goose, duck and chick embryo cells. The size of inoculum and age of embryos used for preparation of primary cultures was decisive for the amount of multiplied virus. One strain (110) of Lednice virus showed reduced ability to induce a cytopathic effect in the first passage in chick embryo cells. Virus multiplication in all three kinds of cells was confirmed by immunofluorescence.

Animals↗
PubMed1976

Studies on viremia and antibody formation in ducklings and goslings after experimental infection with Lednice (Yaba 1) virus.

A very low virus level was found for a short time in the blood of ducklings and goslings after experimental Lednice (Yaba 1) virus infection by subcutaneous (scut) and intranasal (inas) route and in blood of ducklings also after infection by intracerebral (icer) route. Subsequently virus neutralizing antibody formation was examined. Antibody formation was found in most of animals. In goslings onset of antibody formation seems to be slower than in ducklings after the same virus infection dose; vice versa, in ducklings antibody titer decreased after 6 weeks. Using low infection virus dose, neutralizing antibody level in ducklings was lower than in the former case. On examining the blood and the organs, the virus was detected in a very low amount and for a very short time in blood and spleen of goslings and only in blood of ducklings.

Animals↗
PubMed1976

Experimental infection of Macaca mulatta monkeys with Lednice (Yaba 1) virus.

Macaca mulatta monkeys were inoculated with Lednice (Yaba 1) virus strain 6118. The animals developed no clinical signs and the body temperature and values of complete haematological examination remained within normal limits. In spite of positive immunofluorescence in regional lymph nodes, no virus could be recovered from them by passages in mice. Viraemia was not demonstrated. The antibody response was directly proportional to the amount of virus inoculated, i.e. it was significant after a higher virus dose or after repeated inoculation. The results obtained are discussed in respect of and in comparison with serological findings in man.

Animals↗
PubMed1975

Distribution of Lednice (Yaba 1) virus in the chick embryo.

Distribution of Lednice (Yaba 1) virus antigen (LVA) was followed by immunofluorescene (IF) in chick embryos inoculated into the yolk sacs. Positive fluorescence of LVA was observed in neurons and neuroblasts of the developing brain, spinal cord and spinal ganglia as well as in skeletal muscles, heart muscle, vascular endothelium and lung mesenchyma. In the yolk sac, foci of specific fluorescence were occasionally seen in endothelium cells of vessels and in islands of extraembryonic haematopoesis. At sites corresponding to the occurrence of LVA, extensive oedema was accompanied by extravazation of erythrocytes and accumulation of white blood cells. The nature of tissues in which the virus replicates was discussed from the point of view of LVA distribution and the morphological lesions observed.

Animals↗