Controlling TRAIL-mediated caspase-3 activation.
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Adenoviridae↗
Biomedical subjects
D Mérino
Publications and source records attributed to D Mérino.
Structural and biochemical properties of the rat myocardium after 21 days of head-down suspension.
The effects of 21 d of head-down suspension on the biochemical and structural properties of the myocardium were determined in male rats (HDS, n = 10), and compared with control non-suspended animals (C, n = 10). HDS rats were prepared using Morey's tail-suspension model, and maintained at 45 degrees tilt. At the end of the conditioning period, hearts were excised and dissected into right (RV) and left plus intraventricular septum (LV). We observed that HDS rats had lower LV- and RV-absolute weights than C animals (-8.5%, p < 0.05, and -12%, p < 0.05, respectively). The relative ventricle weights (ventricle weight/body weight, mg.g-1) were unaffected by HDS. Native myosin isoform analysis revealed that HDS did not alter myosin expression in both LV and RV. The capillary bed, examined using histochemical methods, was found to be unaffected by HDS. A significant decrease in the lactate dehydrogenase activity was detected in LV after 21 d of HDS (-16%, p < 0.05). Collectively, these results suggest that the early neurohumoral changes occurring in response to HDS-induced hemodynamic overload are sufficient to prevent any alteration in the biochemical and structural properties of the myocardial tissue.
Animals↗
Duration of streptozotocin diabetes influences the response of hypothalamic serotonin metabolism to immobilization stress.
Neurochemical and metabolic effects of acute (immobilization for 2 h) and chronic (immobilization for 2 h/day for 4 consecutive days) stress were investigated in diabetic female rats either pretreated 1 week or 5 weeks earlier with streptozotocin (STZ). Hypothalamic serotonin (5-hydroxytryptamine, 5-HT) metabolism was estimated by measuring the respective levels of 5-HT precursor, the amino acid tryptophan (TRP), 5-HT and the 5-HT metabolite, namely 5-hydroxyindoleacetic acid (5-HIAA). To assess the respective metabolic effects of stress and diabetes, plasma total TRP, insulin, glucose and corticosterone levels were measured. Short- and long-term STZ treatment triggered marked decreases in plasma total TRP and hypothalamus TRP levels but the diabetogenic agent diminished 5-HT metabolism in the 1-week ST-treated rats only. Acute stress promoted a marked decrease in plasma total TRP in the vehicle-treated rats and in the 1-week-diabetic rats, which was associated with significant increases in hypothalamic TRP and 5-HIAA levels. In the 5-week-diabetic rats, a single restraint affected neither peripheral and central TRP levels nor hypothalamus 5-HT metabolism. Acute stress triggered hypercorticosteronemia in all groups of rats but it promoted hyperglycemia and hypoinsulinemia in the vehicle-injected rats only. Twenty-four hours after the fourth immobilization, plasma total TRP was reduced in the vehicle-injected rats only with no effect on hypothalamic levels of TRP. On the other hand, chronic restraint was found to reduce exclusively hypothalamus 5-HT and 5-HIAA levels in the 5-week-diabetic rats.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Feeding responses to a high dose of 8-OH-DPAT in young and adult rats: influence of food texture.
The present study was undertaken to investigate the hyperphagic responses to the 5-HT1A receptor agonist, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), in young and adult rats fed either a powder diet or pellets. In the young rats, 8-OH-DPAT (500 micrograms/kg s.c.) increased the consumption of pellets--but not powder--during the 2 h following drug administration. On the other hand, 8-OH-DPAT did not promote hyperphagia in adult rats presented with either pellets or a powdered diet. The influence of the 5-HT1A agonist on midbrain serotonin (5-hydroxytryptamine, 5-HT) turnover was examined. Administration of 8-OH-DPAT (500 micrograms/kg s.c.) induced similar decreases in 5-HT turnover, as reflected by the ratio of 5-hydroxyindoleacetic acid (5-HIAA) to 5-HT, in young and adult rats 1 h after administration. Nevertheless, some metabolic responses to 8-OH-DPAT were found to be influenced by age. Young and adult rats were injected with a low dose of 8-OH-DPAT (50 micrograms/kg s.c.) to specifically test the presynaptic regulation of 5-HT turnover. Again, midbrain 5-HIAA to 5-HT ratios were decreased to the same extent in both young and adult rats. The results suggest that (i) gnawing may be an important parameter in the food consumption that is triggered by a high dose of 8-OH-DPAT, (ii) analysis of the presynaptic effects of 8-OH-DPAT on 5-HT turnover cannot solely explain the influence of the agonist on feeding behavior.
8-Hydroxy-2-(di-n-propylamino)tetralin↗