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Biomedical subjects

D Man

Publications and source records attributed to D Man.

At least 19 recordsLinked to original sources
PubMed2001

3D H(aro)-NOESY-CH3NH and C(aro)-NOESY-CH3NH experiments for double labeled proteins.

Precision in the determination of the 3D structures of proteins by NMR depends on obtaining an adequate number of NOE restraints. Ambiguity in the assignment of NOE cross peaks between aromatic and other protons is an impediment to high quality structure determination. Two pulse sequences, 3D H(aro)-NOESY-CH3NH and 3D C(aro)-NOESY-CH3NH, based on a modification of a technique for simultaneous detection of 13C-1H (of CH3) and 15N-1H correlations in one measurement, are proposed in the present work. These 3D experiments, which are optimized for resolution in the 13C and 15N dimensions, provide NOE information between aromatic protons and methyl or amide protons. CH2 moieties are filtered out and the CH groups in aromatic rings are selected, allowing their NOE cross peaks to be unambiguously assigned. Unambiguous NOEs connecting aromatic and methyl or amide protons will provide important restraints for protein structure calculations.

Carbon Isotopes↗
PubMed2001

The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery.

The purpose of this study was to evaluate a new technique of harvesting and preparing autologous platelet gel and autologous fibrin glue (body glue) and to evaluate their effectiveness in stopping capillary bleeding in the surgical flaps of patients undergoing cosmetic surgery. A convenience sample of 20 patients ranging from 25 to 76 years of age undergoing cosmetic surgery involving the creation of a surgical flap were included in the study. The types of surgical procedures included face lifts, breast augmentations, breast reductions, and neck lifts. Platelet-poor and platelet-rich plasma were prepared during the procedure from autologous blood using a compact, tabletop, automated autologous platelet concentrate system (SmartPReP, Harvest Autologous Hemobiologics, Norwell, Mass.). The platelet-poor and platelet-rich plasma were combined with a thrombin-calcium chloride solution to produce autologous fibrin glue and autologous platelet gel, respectively. Capillary bed bleeding was present in all cases and effectively sealed within 3 minutes following the application of platelet gel and fibrin glue. The technique for making the solution and for evaluating its effectiveness in achieving and maintaining hemostasis during cosmetic surgical procedures is described. Autologous platelet gel and fibrin glue prepared by the automated concentrate system are compared with autotransfusor-prepared platelet gel and Tisseel (Baxter Healthcare Corp.), a commercially prepared fibrin sealant preparation.

Adult↗
PubMed2000

Sensitivity enhancement of HCACO by using an HMQC magnetization transfer scheme.

Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of (1)H(alpha)-(13)C(alpha)(R(MQ)) is, on average, 1.3 +/- 0.4 or 1.7 +/- 0.6 times slower than the single-quantum relaxation rate of (13)C(alpha)(R(C)) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D(2)O at 20 degrees C.

Algorithms↗
PubMed1999

Community-based empowerment programme for families with a brain injured survivor: an outcome study.

Rehabilitation of brain injured persons has been one of the challenges of the modern health care team. Brain injured persons' problems are termed complex and multiple, and may persist long after the acute management stage. Families caring for their brain-injured members are, however, ill prepared to face this long-term rehabilitation process. A previous study of an empowerment framework applicable among these Hong Kong Chinese families resulted in the development of a 52-item empowerment questionnaire with four interpretable factors (efficacy, knowledge, support and aspiration). This can also serve as a valid and reliable outcome measure of their empowerment efforts. As a follow-up of this development, an 8-week community-based empowerment programme was thus designed and implemented for a total of 50 family members in six repeated groups. Outcome indicators including empowerment questionnaire, psychological well-being, self-efficacy, subjective experience of the burdens in care-giving, and support systems were used to monitor changes in empowerment status during the periods of pre- and post-programme, and during the 3 month follow-up. The programmes were found to be effective in empowering family members in the four postulated empowering dimensions, and improving all other outcome measures. The follow-up studies reflect stability in empowerment, though there were no further improvement. From regression analysis, it was suggested that, for optimum empowerment to take place, important predictors included careers' education levels, age ranges and work status.

Adaptation, Psychological↗
PubMed1999

The influence of permanent magnetic field therapy on wound healing in suction lipectomy patients: a double-blind study.

The authors present their experience with the healing influence of permanent magnets on postoperative wounds. The responses of 20 patients who underwent suction lipectomy and postoperative negative magnetic field therapy were studied in a double-blind fashion. Magnets in the form of patches (10 patients) or sham magnet patches (10 patients) were placed over the operative region in each of the patients. Pain, edema, and discoloration (ecchymosis) were evaluated at 1, 2, 3, 4, 7, and 14 days postoperatively. Our results show that the treatment group had significant reductions in pain on postoperative days 1 through 7, in edema on days 1 through 4, and in discoloration on days 1 through 3 when compared with the control group. These results demonstrated that commercially available magnets have a positive influence on the postoperative healing process in suction lipectomy patients.

Adolescent↗
PubMed1996

A new addition to face lift surgery: the argon gas surgical unit.

In the quest to improve the technique of performing face lift surgery, we have encountered a device, the argon gas surgical unit, that offers a number of advantages over current techniques. These advantages include significant reductions in the amount of both intraoperative and postoperative blood loss, depth of tissue damage, amount of postoperative swelling, and amount of postoperative pain. This paper describes our experience with the use of the argon gas surgical unit compared with conventional electrocautery in 30 patients undergoing face lift. On histologic examination of the skin specimens, there was a consistent decrease in the depth of tissue damage with the argon gas surgical unit. With regard to blood loss, as a whole there was a marked decrease in the argon gas surgical unit group. Additionally, there is a clinically significant decrease in postoperative swelling in the patients in whom the argon gas surgical unit was employed that contributes to improved wound healing and an overall improved postoperative course.

Argon↗
PubMed1989

Stretching and tissue expansion for rhytidectomy: an improved approach.

Intraoperative expansion of the skin of the face supplies additional tissue that permits closure of the face lift incision with minimal tension. This paper presents the findings in rhytidectomy patients over the last 3 years using both intraoperative stretching and intraoperative stretching combined with tissue expansion utilizing the Man face lift expander. Sixty-seven patients underwent rhytidectomy surgery, of whom 50 were treated with stretching techniques alone and 17 were treated with the combined stretching and expansion method. The patients' ages ranged from 28 to 78 years. Results indicate that the patients treated with combined stretching and expansion had significantly more skin removed. This new technique appears to offer significant clinical advantages over usual face lifts.

Adult↗
PubMed1985

Neutral and acidic species of human intestinal mucin. Evidence for different core peptides.

Highly purified human mucins from postmortem intestinal tissue were fractionated on anion exchange columns to generate separate neutral and acidic species. The neutral mucin (less than 1.0 mol % sialic acid) was the major species (greater than 80% by weight) and contained a higher molar proportion of fucose, galactose, and N-acetylglucosamine, and a lower proportion of sialic acid and N-acetylgalactosamine than the acidic species (greater than 10 mol % sialic acid). Amino acid analyses revealed a highly significant enrichment in serine, aspartate, and alanine in the neutral species and proline, threonine, and glycine in the acidic species. Thiol reduction of each species to remove their integral 118,000-dalton component did not alter the essential interspecies differences. Differences in threonine, proline, and serine also remained after removal of all "naked" or pronase-susceptible peptide regions from each species. These results indicate that neutral and acidic mucins contain glycopeptide segments exclusive of their 118,000-dalton and naked peptide components, which differ in amino acid composition. The key amino acid markers are similar to those observed for fuco- and sialoglycopeptides obtained after proteolytic digestion of human colonic mucin by Gold et al. (Gold, D.V., Schochat, D., and Miller, F. (1981) J. Biol. Chem. 256, 6354-6358). The oligosaccharide composition of small intestinal and colonic mucin may therefore depend upon transcriptional control of the synthesis of specific mucin peptides as well as the post-translational activity of glycosyltransferases. These findings may have significance for the quality and functions of mucus produced in a variety of pathological states.

Amino Acids↗
PubMed1984

Abnormal mucus: nominated but not yet elected.

Mucus secretions of the intestine may contribute to plug formation in CF, through several mechanisms acting alone or simultaneously. Excessive mucin secretion and relative dehydration of secretions would elevate mucin concentration and enhance mucus gel formation. Deficient pancreatic enzymes combined with intrinsic hyperglycosylation of mucin or an increase in covalently bound fatty acid groups on mucins, would retard normal proteolytic degradation of mucins. Excessive calcium ions in the ducts would tend to decrease mucin solubility, while excessive hydrogen ions and protein (especially albumin) together with mucins, may be responsible for the appearance of proteinaceous ductal 'plugs' characteristic of CF pathology.

Albumins↗
PubMed1981

Desmoplastic malignant melanoma: a case report.

A further case of desmoplastic malignant melanoma of the cheek is presented, bringing the total number of reported cases to 15. Its clinicopathological features and treatment are discussed.

Cheek↗
PubMed1981

The biceps femoris myocutaneous advancement flap: a useful modification for ischial pressure ulcer reconstruction.

We describe a modification of the biceps femoris myocutaneous flap for reconstruction of ischial pressure ulcer defects. In this modification, all neural and vascular pedicles are preserved and the flap is advanced into the ischial defect rather than transposed as described in the literature. With this method, no transferred tissue is lost. Besides ensuring good tissue survival, the flap preserves a maximum number of reconstructive options in the event of ischial pressure ulcer recurrence. These advantages make the biceps femoris myocutaneous advancement flap our first choice for reconstruction of ischial pressure ulcer defects.

Humans↗