Synthetic peptide combinatorial libraries.
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Antibodies, Monoclonal↗
Biomedical subjects
D Medynski
Publications and source records attributed to D Medynski.
Phage display: all dressed up and ready to role.
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Biotechnology↗
Genetic approaches to protein structure and function: point mutations as modifiers of protein function.
In this review, I summarize data in the biological literature which underscore the utility of a genetic approach to protein structure/function problems, with emphasis on binding phenomena, particularly of cytokine and growth factor/receptor interactions. Useful parallels or contrasts to chemical ligand/receptor systems and DNA binding protein interactions are examined where they simplify the analysis of protein ligand/receptor interactions. This approach was prompted by the fact that purely rational approaches, based on resolution of the three dimensional structure of proteins, are limited because such data is available for fewer than 3% of the 17,000 proteins for which the amino acid sequence has been deduced by molecular biology techniques.
Animals↗
Partial cDNA sequence of the gamma subunit of transducin.
A 151 bp cDNA segment that encodes the amino-terminal portion of the gamma subunit of bovine transducin was isolated from a retinal cDNA library constructed in the expression vector lambda gt11. The base sequence of this cDNA confirms the sequence of the first 39 amino-terminal amino acids reported for the native protein (McConnell et al. (1984) Fed. Proc. 43, 1585). Northern blot analysis indicates that the complete mRNA is approximately 650 bases long and that its expression is limited to the retina.
Amino Acid Sequence↗