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Biomedical subjects

D Micu

Publications and source records attributed to D Micu.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

The metabolic behaviour of the cellular populations in the pleural or peritoneal effusions from patients with malignant diseases.

Complex morphologic, biochemical and cyto-enzymochemical investigations have been performed in the pleural or peritoneal effusions which occurred in 80 patients with various neoplastic diseases (pleural, pulmonary, hepatic, genital or breast cancers). As shown by the morphologic and biochemical tests, the proportion of fluids with a malignant or benignant character varied widely in the different forms of neoplasias. In the same effusions, the authors have studied the metabolic behaviour of acid phosphatase activity, the glycogen and nucleolar RNA amounts in the lymphocytes, in the malignant cells and in the mesothelial ones. High values of these cyto-enzymochemical components were found in the lymphocytes and the malignant cells from fluids with a positive malignant character, although similar changes were also observed in many cells of the effusions with morphologically negative reactions, and in a lesser proportion in those from patients with adenocirrhosis. It is concluded that such investigations could contribute to a better understanding of the metabolic behaviour of the cells from neoplastic effusions thus increasing the possibility of detecting the malignant character of the cells in the pleural or peritoneal effusions with a morphologically negative character.

Acid Phosphatase↗
PubMedDate not supplied

Lymphocyte changes after long-term therapy of some neurological diseases.

Hematologic investigations were carried out in 50 patients with various forms of epilepsy, aged 20 to 50 years, who had been treated for different periods of time with antiepileptic and neuroleptic drugs such as primidon, luminal and prasine. All the cytomorphological and cytochemical studies concerned the white blood cells, especially lymphocytes, and their transformation into plasma cells and histiomonocytes. The cytochemical investigations referred particularly to nucleolar-RNA, glycogen and lipid cellular content. The results obtained showed that the lymphocyte count and mostly that of the large and medium lymphocytes, may reach twice the normal or more. The transition forms towards histiomonocytes and plasma cells were quite frequent. Cytochemically, a variable state of reactivity of the nucleolar apparatus was demonstrated, as well as certain changes of the carbohydrate and lipid metabolism. The influence of such changes on the cellular and humoral immunity of the respective patients is discussed. Emphasis is also laid on the utility of such investigations for the detection of the untoward effects of antiepileptic and neuroleptic drugs on hematopoiesis.

Adult↗
PubMedDate not supplied

Metabolic disturbances of the lymphoid cells and their significance for clinical pathology.

The presentation and discussion of the metabolic disturbances of lymphoid cells is preceded by some brief data on their nomenclature, philogeny, morphology and methodology (some regarding T and B lymphocytes). The most noteworthy results of cytochemical investigations are those concerning cellular biochemical components such as DNA, RNA, glycogen and lipids. Emphasis is layed on the results of optic and electronic cytoautoradiographic investigations. The cytoenzymologic explorations have shown the necessity of determining the activity of certain enzymes such as acid phosphatases, beta-glucuronidase, aryl-sulphatase, succin-dehydrogenase (SDH), lactic and malate dehydrogenases (LDH and MDH), glucose-6-phosphate-dehydrogenase (G-6-PD), DNA- and RNA-polymerases, certain proteases, dihydrofolate-reductase, a.o. The author insists on the value of the data obtained by the above mentioned investigations for the diagnosis, prognosis and therapy of acute and chronic leukemias, various lymphomas, Waldenström's macroglobulinemia, rheumatoid arthritis, chronic hepatitis, systemic lupus erythematosus, myasthenia gravis, a.o.

Cytoplasm↗
PubMedDate not supplied

HBsAg positive secondary autoimmune active chronic hepatitis. Immunogenetic aspects.

Observations were made in a group of 120 patients with active chronic hepatitis (ACH), using demonstration of HBsAg presence, structural study of the peripheral blood mononuclear cell population by means of scanning electron microscopy (SEM) and typing of HLA-A, B and C antigens. From the whole group 22 patients were proved to have an autoimmune form of ACH secondary to infection with hepatitis B virus (HBV). The authors consider that the diagnostic value of the ratio between B and T lymphocytes (studied by SEM) is relative but the demonstration of an increased percentage of monocytomacrophage cells and the presence of "killing phenomenon" clearly reflect a more severe hepatic inflammation. A higher incidence of the HLA-Bw35-Cw4 was observed particularly in the HBsAg positive forms of ACH and in the HBsAg positive secondary autoimmune forms. The authors discuss the utility of these observations when the opportunity of corticotherapy has to be established.

Adult↗
PubMedDate not supplied

Study of the relationship between the granulocyte LDH, alkaline phosphatase and Zn at the level of the leukocyte in patients with chronic myeloid leukemia.

Mixed leukocyte suspensions were prepared from heparinized blood collected from healthy subjects and from patients with chronic myeloid leukemia (CML). In all the suspensions determinations were made for: zinc, by atomic absorption; granulocyte alkaline phosphatase (GAP), using the method with p-nitrophenylphosphatase; granulocyte LDH, by means of the enzymatic autoanalyser LKD 8,600. In the patients with CML, the values of zinc and of granulocyte alkaline phosphatase activity were very low while the granulocyte LDH values were higher than normal. The chromatogram of the granulocyte LDH isoenzymes on DEAE-Sephadex A50 minicolumn (0.5 X 12 cm) showed an "alpha type abnormality" revealed by the increased activity of the isoenzymes with high electrophoretic mobility LDH2 and LDH1 specific for tissues with intense oxidative phosphorylation. In the normal subjects the chromatogram of the leukocyte LDH isoenzymes showed a type M (skeletal muscle) prevalence denoting intense anaerobic glycolysis. Therefore the low zinc concentrations (0.55 micrograms mg N2 as compared with the normal 1.24 micrograms mg N2) in these patients cause the decrease of GAP activity by the lack of zinc in the active center of the enzyme and the decrease of cellular permeability thus allowing the extracellular release of granulocyte LDH.

Alkaline Phosphatase↗
PubMedDate not supplied

Electrophoretogram of lysates deriving from mononuclear cells. Preliminary observation.

Electrophoresis of mononuclear lysates was applied for the first time in the study of the lymphocyte protein content as an additional method beside the rosette test and scanning electron microscopy. The specification of T and B lymphocytes in the presence of discordant results sometimes obtained by the latter methods is facilitated by the additional data supplied by the study of their endoplasmic protein contents. The electfophorogram contributes to a better knowledge of the immunologic status of patients with autoimmune and lymphoproliferative diseases as well as to a more precise determination of the effects following the administration of immunomodulating treatment.

B-Lymphocytes↗
PubMedDate not supplied

Identification and study of the various leukocytes, particularly of the leukemic ones. (Principles and some technical details).

Based on the main data from the specialty literature and on their own experience in the field, the authors present the principles and some details of the classical and the modern techniques used to identify and study the various leukocytes and especially the leukemic ones. These different methods of investigation are included into the wide categories of histologic, cytomorphologic, cytochemical, cytoenzymatic, cytoimmunological, cytogenetic, cell culture and other complementary techniques. The great progress achieved in this domain was favoured by the development of light, contrast phase and fluorescent microscopy and of the new techniques of transmission and scanning electron microscopy--whose utility for the study of the white blood cells, and particularly of the leukemic ones, is demonstrated in this paper by numerous suggestive illustrations.

Bone Marrow Cells↗
PubMedDate not supplied

Normal blood cell values in groups of population from various geographic areas of Romania.

The aim of this work was to determine the normal values of erythro-, leuko-, and thrombocytes in several groups of population situated in various geographic areas (hills, plain, sea-side), considering that such data could be useful for the delimitation between the normal conditions and the near-normal ones. Sixteen hematologic parameters have been investigated in 2,053 healthy subjects, insisting particularly on those most frequently required in the clinic, i.e., erythrocytes/microliter, hemoglobin (g%), hematocrit (%), leukocytes/microliter, and thrombocytes/microliter. No significant differences related to the geographic areas were recorded. The normal values of the hematologic parameters are presented in separate tables.

Altitude↗
PubMedDate not supplied

Examination of human and animal leukemic cells by high contrast scanning electron microscopy.

Investigation of human or animal (sheep and ox) leukemic cells by scanning electron microscopy enabled the identification and study of some ultrastructural details of the cell surface. A more detailed visualization by the high contrast technique of the same cell surface was obtained, in this work, for the first time in the biological field. This technique allows a better interpretation of any zone of the preparation examined, irrespective of the species from which the cells are collected.

Animals↗
PubMedDate not supplied

The value of some cytoenzymochemical investigations of the leukocytes and platelets in estimating the effects of occupational exposure to benzene, vinyl chloride and carbon disulphide.

Cytoenzymochemical investigations were performed on the leukocytes and platelets from 2,782 workers occupationally exposed to benzene, vinyl chloride or carbon disulphide, in view of detecting the eventual occurrence of cytologic metabolic disorders induced by these chemical substances. The cytomorphologic aspects and the cytoenzymochemical parameters studied, such as the leukocyte alkaline phosphatase, endolymphocytic glycogen, lymphocytic nucleolar RNA amounts (revealed by the lymphocyte nucleologram), as well as the platelet counts have shown some abnormal values, whose severity was related to the time of exposure to toxic chemicals. The results obtained demonstrate the utility of such tests for an early detection of some metabolic disorders of the endolymphocytic proteins, carbohydrates or lipids, in view of preventing the occurrence of more severe pathologic consequences.

Acid Phosphatase↗
PubMedDate not supplied

The metabolic behaviour of the lymphocytes from serous effusions in various benign internal diseases.

The functional status of the lymphocytes from pleural or peritoneal effusions occurring in 148 patients with various internal diseases such as tuberculosis, liver cirrhosis, heart failure and pneumonias was studied by laboratory investigations including morphologic examination, cytoenzymatic, cytochemical and biochemical tests for determining the scores of acid phosphatase activity and of endolymphocytic glycogen, as well as the presence of nucleolar RNA. These values were found increased in the fluids from tb patients and in some cases of liver cirrhosis, heart failure and metapneumonic pleurisies. The variations of these parameters are assumed to provide information on the metabolic behaviour of the lymphocytes present in these fluids and on their participation to the immune, inflammatory processes which occur in the course of some of the diseases investigated.

Adult↗
PubMedDate not supplied

Eosinophilia in the pleural and peritoneal effusions.

Of 248 patients with various benign or malignant chronic diseases who presented pleural or peritoneal effusions, the authors have selected 16 cases in which the proportion of eosinophils in these fluids exceeded 5% of the total cell count. High values of eosinophilia have been observed mostly in the serous membrane fluids from patients with tuberculosis and various neoplasms. In the authors' opinion, this suggests the participation of eosinophils in the immune processes occurring in the course of these pathologic conditions, in which the lymphocytes are also involved.

Ascites↗