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Biomedical subjects

D Milo

Publications and source records attributed to D Milo.

26 records · Page 2Linked to original sources
PubMedDate not supplied

Effects of health information in youth on adult physical activity: 20-year study results from the Amsterdam growth and health longitudinal study.

In the Amsterdam Growth and Health Longitudinal Study (AGAHLS), a group of apparently healthy males and females (n = 200) were interviewed about their physical activities on eight separate occasions over a period of 20 years between 13 and 33 years of age (multi-measured group: MM). Information about their health was given based on their personally measured lifestyle (activity, diet, smoking) and biological risk characteristics for chronic diseases (medical check-ups). A comparable group of boys and girls (n = 200) was only measured on two occasions (bi-measured group: BM): at 13 and 33 years. Physical activity was estimated with a structured interview. Total physical activity and sports activity were estimated in three intensity levels (light, moderate, and heavy). It was hypothesized that the eight repeated medical check-ups with health information in the MM group would result in a healthier lifestyle with respect to the determinants and levels of habitual physical activity compared to the BM group. Contrary to the hypothesis, males and females in the BM group showed a significantly higher increase or a lower decrease in physical activities compared to the MM group. This negative effect on the physical activity pattern at 33 years in the MM group may have been caused by more underreporting of physical activities than in the BM group. In conclusion, there does not appear to be a significant effect of long-term (multi-measured) health information with medical check-ups during adolescence and young adulthood on level of physical activity in males and females at 33 years of age.

Adolescent↗
PubMedDate not supplied

Screening and confirmatory tests for antibody to HTLV-III/LAV retrovirus in individuals at risk for AIDS.

The prevalence of specific antibodies to HTLV-III/LAV retrovirus was investigated during 1985 in a large group of subjects at risk for AIDS living in the Florence area. Two hundred and thirty-two of 774 (29.9%) intravenous drug users (IDU), 40 of 164 homosexuals (24.3%), 43 of 147 hemophiliacs (29.2%), 7 of 10 children born from IDU mothers and 4 of 88 heterosexual partners of IDU were found to be seropositive using different ELISA kits. The positivity in all serum samples showing low antibody titres and/or discordant results with different sandwich ELISA kits was confirmed by a competition ELISA assay and the Western blot technique. Serum samples from 4 of 210 hemodialyzed and from one of 17 polytransfused patients also showed positive reactions in the assays based on the sandwich principle, but gave negative results in both the competition ELISA assay and the Western blot technique. In addition, all these sera showed positive reactions using immunofluorescence and ELISA procedures that control for reactivity with H9 human cell line material used for culturing the HTLV-III/LAV retrovirus. These data demonstrate that exposure to HTLV-III/LAV retrovirus is widespread in groups of subjects at risk for AIDS living in the Florence area. Furthermore, the results of the present study suggest that, in addition to the Western blot technique, the competition ELISA assay as well as assays that control reactivity with H9 human cell line material may be of value for detecting false positive reactions due to antibody cross-reactive with human cellular components.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Proctocolitis caused by Schistosoma mansoni in AIDS. A clinical and immunohistochemical study of a case].

An imported case of intestinal schistosomiasis in AIDS is analysed by the relationships between immune response, diagnostic methods, pathogenetic mechanisms. The inconclusiveness of negative results from both serology and stool parasitology is remarked whereas the efficacy of associated histological, histochemical and immunohistochemical methods is emphasized in order to get better data on aetiological diagnosis and on cell types and size of reactive granulomas. In the case studied, negative serology, absent fecal egg excretion and uneffective granulomatous response with scarcity of T and B lymphocytes have been documented by means of the above methods.

Acquired Immunodeficiency Syndrome↗