PubMed Health⌕ Search

Biomedical subjects

D Milosavljević

Publications and source records attributed to D Milosavljević.

10 recordsLinked to original sources
PubMed1980

Interaction between adenosine triphosphate and noradrenaline in the isolated vas deferens of the guinea-pig.

1 An interaction between adenosine triphosphate (ATP) and noradrenaline (NA) was investigated in the isolated vas deferens of the guinea-pig. 2 ATP (1.7 to 50 microM) enhanced contractions due to transmural electrical stimulation (ES; parameters: 30 Hz, 0.2 to 0.3 ms, 10 to 30 V; 1 s train duration). Responses to exogenous NA (12 microM) were also potentiated by ATP while contractions to acetylcholine (6 microM) were inhibited. 3 NA (1.2 microM) potentiated ATP-induced contractions and prevented the development of tachyphylaxis to ATP (510 microM). 4 Phentolamine (12.5 microM) prevented the potentiation by NA of ATP-induced contractions; these contractions were insensitive to phentolamine (up to 25 microM). 5 Removal of potassium chloride from the Tyrode solution for 10 min abolished the potentiating actions of both ATP and NA. 6 The present results suggest that the effect of ATP may be functionally closely related to that of NA at alpha-adrenoceptors in the vas deferens of the guinea-pig.

Adenosine Triphosphate↗
PubMed1977

Influence of pyridylisatogen tosylate on contractions produced by ATP and by purinergic stimulation in the terminal ileum of the guinea-pig.

2-2'Pyridylisatogen tosylate (PIT), which antagonizes the inhibitory action of ATP in the taenia caeci, did not antagonize the excitatory effects of exogenous ATP and of purinergic stimulation of the terminal guinea-pig ileum. PIT (0-5--2-5 muM) potentiated the ATP-induced contractions and also the contractions produced by potassium chloride, though the potentiation could not be related to the dose in every experiment. The responses to noradrenaline, adrenaline and histamine were slightly inhibited. PIT also potentiated the contractions produced by electrical stimulation of intramural purinergic nerves when either an alternate or uniform stimulation pattern was used. The present results in which the preparation is contracted by ATP are opposite to those obtained with PIT on the taenia caeci, which is relaxed by ATP. This raises a question of duality or plurality of receptors for ATP.

Adenosine Triphosphate↗
PubMed1976

Influence of adenosine, cAMP and db-cAMP on responses of the isolated terminal guinea-pig ileum to electrical stimulation.

Adenosine, cAMP and db-cAMP were found to inhibit nerve-mediated contractions of the terminal ileum elicited by electrical stimulation (ES). Responses to ES at 30 Hz, which are believed to be of adrenergic origin, were considerably more affected than the responses to ES at 3 Hz, which may be of purinergic orign. The following order of relative inhibitory potencies was established: adenosine greater than cAMP greater than db-cAMP. Adenosine, cAMP and db-cAMP did not affect the contractile responses of the preparation to histamine, potassium and noradrenaline. Therefore, the observed inhibition of nerve-mediated contractions was thought to be due to an action on presynaptic sites, i.e. on the neural network within the intestine. This action was antagonized by an increase in external calcium and by aminophylline, which is known to increase intracellular calcium. The present experiments suggests that the inhibitory action of adenosine, cAMP and db-cAMP results from a reduction of calcium fluxes across the neural membrane and to a subsequent reduction in the release of neurotransmitter(s). The inhibitory effect of cAMP appeared to be nonspecific and was presumably related to adenosine in its molecule.

Adenosine↗
PubMedDate not supplied

[Personal experience in the treatment of pulmonary tuberculosis in mentally retarded persons].

The results of the therapy of pulmonary tuberculosis in 90 mentally retarded patients in various forms and degrees of retardation are presented. General and clinical condition of 62 patients was very bad prior to the onset of antituberculotic therapy and most of them were immobile due to the mental retardation and dissemination of pulmonary tuberculosis. In 30 patients advanced form of tuberculosis, in 54 patients mild form and in 6 patients a minimal degree of tuberculosis were found. In hospitalized patients all clinical forms of pulmonary tuberculosis were found. The therapy started with triplicate antituberculotic therapy and the patients were also administered other drugs due to their mental retardation. During the first two months of antituberculotic therapy a high mortality rate was registered (27 or 30%) especially in younger patients. The reason for such high rate cannot be attributed only to pulmonary tuberculosis but also to the primary disease as was the main reason of patients' hospitalization at the institute for mentally retarded persons. Many complications in antituberculotic use could not be registered since many of the basic data could not be obtained from mentally retarded subjects. The complete recovery or improvement of pulmonary tuberculosis was achieved in 63 patients.

Adult↗