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Biomedical subjects

D Mlynarcík

Publications and source records attributed to D Mlynarcík.

33 records · Page 2Linked to original sources
PubMed1981

The effect of quaternary ammonium compounds and amine oxides on spores of Bacillus cereus.

The effect of 1-dodecylpiperidine 1-oxide and N,N'-bis(dodecyldimethyl)-1,2-ethane diammonium dibromide on the spores of Bacillus cereus. particularly their binding to intact spores and spores with reduced cystine bonds, was investigated. The Langmuir type of binding is involved in both cases. Both compounds decreased the thermoresistance of spores. DPNO decreased the fraction of non-germinating spores, the effect of the drug increasing with increasing concentration. This phenomenon was associated with a faster release of dipicolinic acid to the medium. Only microgermination proceeded in the presence of BDED and dipicolinic acid was released only in substantially lower amounts. Both compounds also influenced respiration.

Bacillus cereus↗
PubMedDate not supplied

Immunomodulatory activity of amphiphilic antimicrobials on mouse macrophages.

A quaternary ammonium salt (1-methyldodecyl)-trimethylammonium iodide, a structurally analogous amine oxide (1-methyldodecyl)-dimethylamine-N-oxide and the amine oxide lacking long alkyl chain trimethylamine-N-oxide were tested for their immunomodulatory activity. Inbred mice strain C57/BL6 were pretreated for 7 days by the compounds under study. The activity of elicited peritoneal macrophages was also tested. Both compounds have a long alkyl chain. In concentrations of 10(-6) M there was a significant increase of the phagocytic, candidacidal and lysozyme activities of the cells. We also observed a suppressed peroxidase activity. The colicidal activity of both the peritoneal and spleen cells were not affected. The amine oxide lacking the long alkyl chain has the same effect at high concentration. A similarity between the effects of the amphiphilic compounds on the macrophages and their antimicrobial efficacy elicits the conclusion that both activities are caused by their ability to interact with the cell membranes.

Adjuvants, Immunologic↗
PubMedDate not supplied

Antimicrobial activity of quaternary ammonium salts of some saturated heterocycloalkyl amines [1].

Antimicrobial activity of N-alkyl-N-dodecylpiperidinium bromides and N-ethyl-N-dodecylheterocycloalkyl ammonium bromides (pyrrolidine, morpholine, perhydroazepine) determined on grampositive and gramnegative bacteria, yeasts and moulds, presented as minimum inhibition concentration (MIC). Comparison of the effect of change of structure: lengthening of alkyl chain, change of heterocyclic ring. Change in the length of alkyl chain markedly affects the antimicrobial activity, change of heterocyclic ring has no substantial effect. The most active compounds were N-heptyl-and N-hexyl-N-dodecylpiperidinium bromides.

Anti-Bacterial Agents↗