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Biomedical subjects

D Monroe

Publications and source records attributed to D Monroe.

At least 19 recordsLinked to original sources
PubMed1999

Dehydroepiandrosterone and related steroids alter 3T3-L1 preadipocyte proliferation and differentiation.

The purpose of the present study was to determine if the anti-adipogenic effects of dehydroepiandrosterone (DHEA) are mediated solely by DHEA or by one or more of its downstream metabolites. In Experiment 1, preconfluent proliferating cultures of 3T3-L1 preadipocytes were incubated for either 24 or 72 h with 0, 1, 5 or 25 microM DHEA, DHEA sulfate (DHEAS), testosterone, estrone and 17beta-estradiol. Pregnenolone, a precursor of DHEA(S), was also tested at these concentrations. After 24 h of incubation, DHEAS, 17beta-estradiol and estrone at the 1 microM level stimulated preadipocyte proliferation. In contrast, DHEA and 17beta-estradiol at the 25 microM level attenuated proliferation to a greater extent than all other steroids. After 72 h of incubation, DHEA and 17beta-estradiol at the 25 microM level attenuated proliferation to a greater extent than all other steroids. In Experiment 2, post-confluent cultures of differentiating 3T3-L1 preadipocytes were incubated for 6 days with 0, 5, 30, or 60 microM levels of these steroids. Preadipocyte differentiation, as assessed by lipid content and glycerol-3-phosphate dehydrogenase activity, decreased markedly when treated with 30 and 60 microM DHEA, 17beta-estradiol, estrone and pregnenolone. In contrast, DHEAS had no impact on preadipocyte proliferation or differentiation. These results suggest that the anti-adipogenic actions of DHEA in adipose tissue may be mediated, in part, by one or more of its distal metabolites, including 17beta-estradiol.

3T3 Cells↗
PubMed1990

Previous donor pregnancy as a risk factor for acute graft-versus-host disease in patients with aplastic anaemia treated by allogeneic marrow transplantation.

To determine if previous donor pregnancies influence the development of acute graft-versus-host disease (GVHD) we evaluated data from 136 patients with aplastic anaemia greater than 15 years of age and given marrow grafts from HLA-identical sibling donors. Of the 136 marrow donors, 30 were parous females (previous history of pregnancy), 30 were nulliparous females (no history of pregnancy or abortions), and 76 were males. The cumulative incidence of grade II-IV GVHD was 57%, 21% and 46% for patients with parous, nulliparous and male donors, respectively. A multivariate analysis of the data confirmed that the risk of grade II-IV acute GVHD was significantly increased among patients receiving marrow from parous females as compared to those from nulliparous females (relative risk = 2.5, P = 0.02). There was no statistically significant difference in the incidence of acute GVHD, however, between patients with parous donors and male donors (relative risk = 1.3, P = 0.26). Male patients given grafts from parous donors showed a higher incidence of acute GVHD (63%) than female patients (45%), though this difference was not statistically significant. The 5-year probability of survival was 47% for patients with parous donors, 68% for patients with nulliparous donors and 70% for those with male donors. We confirm that prior donor pregnancy represents an important factor in selecting marrow donors or designing clinical protocols for GVHD prophylaxis.

Acute Disease↗
PubMed1990

Amperometric immunoassay.

An exciting new analytical technique based on amperometric devices used to measure immunoassays is reviewed in this article. The utility, ease, speed, simplicity, and many other advantages of performing amperometric immunoassays (AIAs) are discussed throughout the review. Limitations are also described, with electrochemical comparisons being made between AIAs and other conventional analytical methods, including potentiometric immunoassays. The review essentially consists of a general AIA overview, followed by sections devoted to amperometric electrode types, assay design, basic principles, automation, application, and future use. Operational features of oxygen gas selective electrodes and oxidoreductase probes are discussed for a better understanding of AIA principles. Characteristics, limits, advantages, and disadvantages of these different devices are presented. A variety of homogeneous and heterogeneous AIAs are described, together with many applications of the different assay formats available. AIAs are classified according to the electrode type employed, enzyme labels required, or electrochemical components involved. Important related AIAs discussed include pulse agglutination reactions and bioaffinity or displacement assays.

Affinity Labels↗
PubMed1989

Potentiometric (bioselective electrodes) assay systems: utility and limitations.

Numerous potentiometric assays utilizing bioselective electrodes are fast revolutionizing many areas of biotechnology. Adequately discussing the utility and limitations of these electrochemical systems is the purpose of this review. A general overview introduces bioselective potentiometry by presenting basic concepts, historical background, and current developments. Essentially, the review consists of several sections describing electrode architecture, operational concepts, different biosensors, assay systems, applications, and future trends. Advantages and disadvantages of the different bioselective assay systems discussed are included throughout each section. Electrode design discussion covers conventional liquid probes and the newer solid-state transitor biosensors. Limitations and advantages of different chemoreceptors, biocatalysts, and potentiometric transducers are presented. Operational characteristics include: linear behavior, sensitivity, stability, specificity, response, recovery, and the influence of interfering factors. Enzyme, organelle, tissue, and microbial biocatalytic sensors are discussed. Bioligand systems include: affinity, immunoselective enzyme, and liposome sensors. Potentiometric bioselective drug, microbial, and immunoassay systems are also included.

Electrodes↗