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Biomedical subjects

D Mottet

Publications and source records attributed to D Mottet.

18 recordsLinked to original sources
PubMed2006

Non-linear analyses of heart rate variability during heavy exercise and recovery in cyclists.

We investigated the time course of RR interval variability during exercise and subsequent 50 minutes of recovery in seven well-trained male cyclists who performed an exercise with 3 successive 8 min stages at 40 %, 70 % and 90 % of their maximal oxygen uptake. The goal of the study was to check whether the decrease in the amplitude of heart rate variability during heavy exercise was accompanied by changes in the chaotic structure of the fluctuations. Heart rate variability was analysed in the temporal and frequency domain using traditional tools and using non-linear methods (Largest Lyapunov Exponent, Detrended Fluctuation Analysis, Minimum Embedding Dimension). When compared to rest, variability at the heaviest exercise intensity was significantly lower (RR: 0.94 +/- 0.22 vs. 0.34 +/- 0.01 ms; SDRR: 0.11 +/- 0.04 vs. 0.01 +/- 0.00 ms) due to a decrease in both LF (2101 +/- 1450 vs. 0.14 +/- 0.09 ms (2) . Hz (-1)) and HF spectral energy (1148 +/- 1126 vs. 7.88 +/- 9.24 ms (2) . Hz (-1)). Non-linear analyses showed that heart rate variability remained chaotic whatever the exercise intensity (the largest Lyapunov exponent was positive at 90 % of the maximal oxygen uptake), with a fractal organisation that tended towards white noise (DFA value close to 0.5) during heavy exercise. During recovery, temporal and spectral variables came back to their rest values within about 30 minutes following an exponential pattern. Non-linear analyses revealed that heartbeat dynamics were disorganised at the beginning of recovery, and involved more regulating systems than at rest, even after 50 minutes of recovery. We concluded that, during heavy exercise, heart rate variability was mainly influenced by other factors than autonomous nervous system, and suggest that mechanical or neurological couplings between the cardiac, locomotor and respiratory systems could play an important part in the observed changes.

Adult↗
PubMed2004

Stability and phase locking in human soccer juggling.

We explored a variant of juggling in which human adults were asked to rhythmically bounce a soccer ball with their dominant foot while standing on the other foot. Eight subjects performed the task at three prescribed heights and one free height condition. Kinematic analyses of foot movement at ball-foot impact showed that, for the smallest height, foot acceleration was positive or zero at impact, which indicates an active stabilization regime. Increasing juggling height resulted in foot acceleration becoming increasingly negative at impact, which is required for a passive dynamical stability regime. These results show that skilled soccer jugglers exploit the passive stability regime afforded by the task, but that similar stability can be achieved with an active stabilization strategy.

Adult↗
PubMed2001

Transduction pathways involved in Hypoxia-Inducible Factor-1 phosphorylation and activation.

Hypoxia-Inducible Factor-1 (HIF-1) is a transcription factor which is activated by hypoxia and involved in the adaptative response of the cell to oxygen deprivation. During hypoxic stress, HIF-1 triggers the overexpression of genes coding for glycolytic enzymes and angiogenic factors. To be active HIF-1 must be phosphorylated. HIF-1 is a substrate for various kinase pathways including PI-3K and the MAP kinases ERK and p38. Several transduction pathways have been proposed which act downstream of putative oxygen sensors and lead to the activation of these kinases. In this review, we summarize some of the latest advances describing the possible signaling pathways leading to HIF-1 phosphorylation and subsequent activation. The physiological relevance of these regulations is also discussed.

Angiogenesis Inhibitors↗
PubMed2001

c-JUN gene induction and AP-1 activity is regulated by a JNK-dependent pathway in hypoxic HepG2 cells.

Hypoxia is an important pathophysiological stress that occurs during blood vessel injuries and tumor growth. It is now well documented that hypoxia leads to the activation of several transcription factors which participate in the adaptive response of the cells to hypoxia. Among these transcription factors, AP-1 is rapidly activated by hypoxia and triggers bFGF, VEGF, and tyrosine hydroxylase gene expression. However, the mechanisms of AP-1 activation by hypoxia are not well understood. In this report, we studied the events leading to AP-1 activation in hypoxia. We found that c-jun protein accumulates in hypoxic HepG2 cells. This overexpression is concomitant with c-jun phosphorylation and JNK activation. Moreover, we showed that AP-1 is transcriptionally active. We also observed that AP-1 transcriptional activity is inhibited by a MEKK1 dominant negative mutant. Moreover, the MEKK1 dominant negative mutant as well as deletion of the AP-1 binding sites within the c-jun promoter inhibited the c-jun promoter activation by hypoxia. All together, these results indicate that, in hypoxic HepG2 cells, AP-1 is activated through a JNK-dependent pathway and that it is involved in the regulation of the c-jun promoter, inducing a positive feedback loop on AP-1 activation via c-jun overexpression.

Cell Hypoxia↗
PubMed2001

The dynamics of rhythmical aiming in 2D task space: relation between geometry and kinematics under examination.

We explored a two-dimensional task space variant of the classical rhythmical Fitts' task in which participants were asked to sequentially cross four targets arranged around the extreme points of the major axes of an ellipse. Fitts' law was found to adequately describe the changes in movement time with the variations in task difficulty (ID), but the 1/3 power-law relating curvature and tangential velocity of the trajectory did not resist the increase in ID. Kinematic analyses showed that the behavioral adaptation to the ID resulted in an increase in the contribution of non-linear terms to the kinematics along the two axes of task space. Moreover, a limit cycle model (combining Rayleigh damping and Duffing stiffness, as in one-dimensional Fitts' task) captured such a behavior. In such a context, Fitts' law and the 1/3 power law appear as surface relations that emerge from parametric changes in a dynamical structure that captures the nature of Fitts' task.

Adaptation, Psychological↗
PubMed2001

Two-handed performance of a rhythmical fitts task by individuals and dyads.

The authors investigated 4 variants of a reciprocal Fitts task in which the pointer was moved to a stationary target, the target was moved to a stationary pointer, or both the pointer and the target were moved to each other bimanually; the bimanual task was carried out either by a single person or by a dyad. Fitts's law held in all 4 conditions, with only minor parametric changes. The kinematic organization varied with task difficulty but remained invariant in task space (i.e., in the mutual frame of reference of the pointer-target system) whatever the pointing condition. In the bimanual conditions, the 2 effectors were coordinated in antiphase with compensatory variability. The authors suggest that the observed chronometric and kinematic patterns emerge from an interplay between simple harmonic motion and the stabilizing influence of the informational flow generated by the closing of the gap between the pointer and the target interval.

Adult↗
PubMed2001

HIF-1 and AP-1 cooperate to increase gene expression in hypoxia: role of MAP kinases.

HIF-1 is the main transcription factor responsible for increased gene expression in hypoxia: VEGF, erythropoietin, GLUT-1, and glycolytic enzymes are such target genes and all participate in the adaptative response of cells to hypoxia. AP-1 activation by hypoxia has also been demonstrated in several cell lines and it cooperates with HIF-1 for increasing VEGF gene transcription in hypoxia. Both HIF-1 and AP-1 activation by hypoxia seems to involve members of the MAP kinase family. Here, we summarize the data indicating that ERK and JNK are needed for activation of HIF-1 and AP-1, respectively.

Animals↗
PubMed2000

The dynamics of human isometric pointing movements under varying accuracy requirements.

The goal of the present study was to explore the relation between speed-accuracy trade-off phenomena and action kinematics in the case of pointing under isometric conditions. Increasing task difficulty resulted in a linear increase in movement time (as predicted by Fitts' law) and in systematic changes in the spatio-temporal patterning of force production. The observed changes in motion topology were similar to those reported for isotonic tasks and adequately captured by a limit cycle model derived for the latter type of task. These results indicate that, for isometric force control as for isotonic position control, the reasons underlying the emergence of Fitts' law might be sought in dynamic trajectory formation processes.

Adult↗
PubMed2000

ERK activation upon hypoxia: involvement in HIF-1 activation.

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1alpha is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia.

Blotting, Western↗
PubMed1999

Hypoxia-induced activation of HIF-1: role of HIF-1alpha-Hsp90 interaction.

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear translocator (ARNT)-Sim (PAS) factors Sim and aryl hydrocarbon receptor (Ahr). The transcription factor hypoxia-inducible factor-1alpha (HIF-1alpha), involved in the response to hypoxia, also belongs to the bHLH-PAS family. This work was aimed to investigate the putative role of Hsp90 in HIF-1 activation by hypoxia. Using a EGFP-HIF-1alpha fusion protein, co-immunoprecipitation experiments evidenced that the chimeric protein expressed in COS-7 cells interacts with Hsp90 in normoxia but not in hypoxia. We also demonstrated that Hsp90 interacts with the bHLH-PAS domain of HIF-1alpha. Moreover, Hsp90 is not co-translocated with HIF-1alpha into the nucleus. At last, we showed that Hsp90 activity is essential for HIF-1 activation in hypoxia since it is inhibited in the presence of geldanamycin. These results indicate that Hsp90 is a major regulator in HIF-1alpha activation.

Animals↗
PubMed1999

The dynamics of goal-directed rhythmical aiming.

Based on the kinematics of goal-directed aiming movements in a reciprocal Fitts' task, a minimal limit cycle model is proposed that is capable of producing the behavior observed at levels of task difficulty ranging from 3 to 7. From graphical and statistical analyses of the phase planes, Hooke's planes and velocity profiles, we concluded that the minimal terms to be included in the model were (i) a nonlinear damping in the form of a self-sustaining, velocity-driven Rayleigh oscillator and (ii) a nonlinear stiffness in the form of a softening spring Duffing term. The model reproduced the kinematic patterns experimentally observed in rhythmical precision aiming, accounting for 95% of the variance. The coefficients in the model changed in a systematic way when distance and precision constraints were varied, and the meaning of these changes is discussed in the framework of the dynamical patterns approach.

Adult↗
PubMed1998

Trajectory formation and speed-accuracy trade-off in aiming movements.

The trade-off between speed and accuracy and the patterning of movement kinematics have been central issues for theories of human movement for almost a century. In the present contribution experimentally obtained kinematics of reciprocal aiming movements, performed under different task conditions, are modelled as resulting from a single non-linear dynamical system whose parameters vary so as to respond to the task demands. Providing a unified account of speed-accuracy trade-off and trajectory formation phenomena, the model offers a theoretical framework in which both discrete and continuous movements, performed along one or more dimensions can be understood.

Biophysical Phenomena↗
PubMed1996

Energy cost and stride duration variability at preferred transition gait speed between walking and running.

The aim of this study was to explore the relationship between energy cost of locomotion and stride duration variability in the region of the walk-run transition speed. Ten subjects participated in this experiment during four treadmill sessions. The first session was used to habituate subjects to treadmill walking or running. During the second session the treadmill speed was increased from 6 km.h-1 to 10 km.h-1 in steps of 0.2 km.h-1 to determine the freely chosen transition speed between walking and running (ST). The last two sessions consisted of five walks or five runs conducted at five intensities selected to represent respectively ST-1 km.h-1; ST-0.5 km.h-1; ST; ST + 0.5 km.h-1; and ST + 1 km.h-1. Exhaled gases were collected during the last two sessions, and stride duration was continuously recorded. The results indicated a significant increase in stride duration variability before ST for the freely chosen gait condition only. These findings point to an alternative hypothesis to that classically proposed to describe a transition as an energy saving mechanism.

Adaptation, Physiological↗
PubMed1994

Fitts' law in two-dimensional task space.

A traditional continuous Fitts' task may be described as a one-dimensional oscillation between two targets. The combination of two such oscillations along intersecting axes gives rise to a two-dimensional aiming task, allowing the study of the speed-accuracy trade-off in two-dimensional task space. In two experiments subjects were asked to draw as many ellipses as possible while passing through four targets, arranged around the extreme points of the two major axes of a model ellipse. In the first experiment, task difficulty was manipulated simultaneously along the two axes of the ellipse. Regardless of ellipse eccentricity and orientation, movement time (MT) was found to depend linearly on Fitts' index of difficulty (ID), which combines between-target distance and target width. In the second experiment, ID was manipulated independently for the short and the long axes of the ellipse. There was a strong linear relation between MT and ID averaged over the two axes, with the two independent measures of task difficulty exerting interactive effects on MT: the higher the ID on one axis, the smaller the effect of the ID on the other. The present results demonstrate that Fitts' law, only examinated so far in one-dimensional aiming tasks, generalises to two-dimensional task space.

Adult↗
PubMed1994

A note on data smoothing for movement analysis: the relevance of a nonlinear method.

The goal of this experiment was to test a potentially useful nonlinear method for smoothing noisy position data, which often is encountered in the analysis of data. This algorithm (7RY) uses a nonlinear smoothing function and behaves like a low-pass filter, automatically removing aberrant points; it is used prior to differentiation of time series so that usable acceleration information can be obtained. The experimental procedure comprises position data collection along with direct accelerometric data recording. From the position-time data, (a) 7RY and (b) Butterworth algorithms have been used to compute twice-differentiated acceleration curves. The directly recorded acceleration measurements were then compared with the acceleration computed from the original position data. Although the results indicated an overall good fit between the recorded and the calculated acceleration curves, only the nonlinear method led to reliable acceleration curves when aberrant points were present in the position data.

Journal Article↗
PubMed1992

[Training in motor skills and psychopathology. Climbing with psychotic children].

The author investigates learning skills in psychotic and borderline children through the physical activity of climbing. Motor skills are considered as an interaction between the subject and his/her environment. Motor learning skills have been studied throughout 6 training sessions in rock-climbing. Etho-clinical methods of investigation allow to assess behaviors and the meaning given by the subject to different forms of action. The author emphasizes here the pleasure/anxiety dimensions of this activity. Results show remarkable learning skills in these children. Significant increase in motor activity and in the height reached point to an obvious integrity of the mechanisms necessary to motor acquisitions. These results are closely linked with the characteristics of rock climbing, a sport involving clinging behaviors and leading to a mastering of the primary anxiety of loosing support.

Child↗
PubMed1990

[Prevention of thromboembolism using low molecular weight heparin and hemodilution in traumatologic geriatric surgery of the femur neck].

A routine method of prophylaxis of thromboembolic disease is evaluated on 181 elderly patients undergoing post-traumatic hip surgery. A low incidence of proximal thrombosis and fatal embolism is noted and rated as a beneficial effect of the association of low molecular weight heparin therapy and moderate hemodilution; it is also connected with a precocious diagnosis of the thrombotic complications--before the proximal extension of silent thromboses--by the measurement of specific fibrin derivatives used as a screening technique in order to select the patients who will have to undergo venographical examination.

Aged↗