Histochemistry of alkaline phosphatase in kidney tissue culture cells: a case for enzyme induction.
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Biomedical subjects
Publications and source records attributed to D N Kamat.
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Five fish species living in different habitats, i.e. fresh water, estuarine and marine, were studied for the distribution of PAS-positive materia in various regions of the kidney, 10 minutes' oxidation with 0-5 per cent. HIO4 being employed prior to treatment with Schiff's reagent. PAS-positive material was detected in different sites of the kidney, i.e. brush border of proximal tubules, proximal tubule cells' cytoplasm, distal tubule cells' cytoplasm, glomerulus, basal cell border of proximal tubules and the interstitial cells. Of these sites, the brush border of the proximal tubule of Scoliodon sorrakowah showed the presence of PAS-positive lipids. Elsewhere the PAS-positive reaction was due to carbohydrates. Free aldehyde groups were absent. In Tilapia mossambica and Labeo rohita, PAS staining was enhanced after chloroform-methanol extraction, particularly in the brush border of the proximal tubule. The significance of these findings is discussed.
Six fish species living in different habitats such as fresh water, estuarine, and marine were studied for the distribution of phospholipids in various regions of the kidney. Well known histochemical methods, including those for choline-containing phospholipids were employed. The distribution pattern of phospholipids in different parts of the kidney such as the brush border, basal border and cytoplasmic granules of the proximal tubule cells, the cytoplasmic granules of the distal tubule cells, glomeruli and interstitial cells is given. Choline-containing phospholipids are present in the brush border of the proximal tubules of all the species studied and in the basal border of some. In the discussion an attempt at correlation of this histochemical pattern with the excretory functions and osmoregulation in these fishes is given.
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