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D N Nosik

Publications and source records attributed to D N Nosik.

At least 19 recordsLinked to original sources
PubMed2004

[Effect of novel natural hypolipidemic compounds on human immunodeficiency virus].

Target screening among microbial products resulted in isolation of hypolipidemic compounds tested for activity against HIV in culture of transferable lymphoblastoid cells MT-4. The majority of the compounds showed antiviral activity. The highest antiviral effect was observed when before exposure to the virus the cells were preincubated for 1 hour in the presence of the isolated compounds. The compounds showed no effect when added to the cell culture preliminarily infected by HIV.

Anti-HIV Agents↗
PubMed1994

[The dependence of the biological activity of metal complexes on the structural stability of the initial DNA].

The authors have formulated the basic principles of the creation of metallo-constructions with DNA which possess biological activity. Thus, the interaction of metal and DNA during formation of metallo-construction must not destabilize the structure of initial DNA. The studies conducted by the authors prove the hypothesis of the working mechanism of these complexes: pronounced activity against viruses which contain DNA and RNA.

Antiviral Agents↗
PubMed1992

[The immunoenzyme test system for detection of HIV-1 antigens based on using immune polyclonal anti-HIV serum and monoclonal antibodies against gene GAG HIV-1 proteins].

The paper describes the enzyme immunoassay system for detection of human immunodeficiency virus antigens, which is based on the use of rabbit anti-HIV antibodies and monoclonal antibodies to HIV-1 gene proteins gag. The system may be useful in the examination of laboratory and clinical samples to reveal both free and conjugated antigens in the composition of immune complexes. The sensitivity of the assay system under development is 0.5 ng/ml at 100% specificity.

Acquired Immunodeficiency Syndrome↗
PubMed1992

[The characteristics of the HIV isolated from HIV-infected persons and AIDS patients on the territory of the CIS].

HIV strains were isolated from HIV-infected patients and AIDS patients in CIS. A total of 81 HIV isolates were obtained. The isolates were identified by using immunofluorescence and enzyme immunoassay, by determining the activity of reverse transcriptase, immunoblot, electron microscopy, polymerase chain reaction. Of the 81 isolates 79 were HIV-1 and 2 HIV-2. The strains differed in their infectivity, the kinetics of virus antigen accumulation, and the spectrum of susceptible cell lines. The viruses isolated may be assigned as two groups: high and low infective. The biological properties of the national HIV isolates were shown to be similar to the prototype HIV strains isolated elsewhere.

Acquired Immunodeficiency Syndrome↗
PubMed1991

Karyological approach to the identification of true cell lines susceptible to the human immunodeficiency virus (HIV).

A karyological analysis of twenty-two variants of eight cell lines, which differed in their susceptibility to the human immunodeficiency virus (HIV) and which had been obtained from different sources, was carried out by means of differential chromosome staining for G and C bands. The karyotypes of eight T-lymphoblastoid cell lines were identified, including five (MT-4, Molt-3, CEM, H-9, and Hut-78) not previously studied by cytogenetic methods. Karyotyping confirmed the identity of seventeen variants of the eight cell lines, and five variants of four lines were found to be misidentified. Comparative analysis of the cytogenetic characteristics of the three CEM-line variants demonstrates the need for karyotype evaluation in the course of in vitro cell cultivation. Fourteen identical marker chromosomes were revealed in H-9 and Hut-78 cell karyotypes, confirming the common origin of these two lines. It was found that the cells of the HIV-susceptible lines had a tendency to undergo polyploidisation both during the initial stages after isolation and in the course of cultivation.

Cell Line↗
PubMed1988

[Biological activity of human alpha-interferons studied using specific antisera].

Preparation of highly active rabbit antisera (AS) to human recombinant alpha 2-interferon and their use for studying biological properties of natural and plasmid alpha-interferons are described. By exhaustion of AS by alpha 3-interferon there were prepared practically monospecific AS not reacting with antigenic determinants of alpha 3-interferon. It was found that alpha 3-interferon represented a significant portion of human lymphoblastoid interferons and was included in PH-labile alpha-interferon from serum of patients with Kaposi carcinoma. AS to alpha 2-interferon completely neutralized antiviral and antiproliferative activity of the homologous subtype alpha-interferon and stimulation of cytotoxicity of human natural killer cells induced by it. It neutralized also the same effects of the heterologous subtypes (alpha 3 and alpha F/D) and leukocytic interferon, but the neutralization level was lower. The results of the study confirmed the polyfunctional nature of the interferon molecule.

Animals↗
PubMed1987

[Antibodies reacting with the antigens of the human immunodeficiency virus in systemic lupus erythematosus].

A study was made of the level of anti-HTLV-111 using an enzyme immunoassay (Emzygnost-anti-HTLV-111) in 75 donors and 143 patients with various diseases including SLE (57), rheumatoid arthritis (9), Sjögren's disease (3), Bekhterev's disease (2), polymyositis (2), dilatation cardiomyopathy (30), and acute lymphocytic leukemia (40). Anti-HTLV-111 were detected in 26 SLE patients and in one patient with polymyositis. A study of positive sera using immunoblotting showed no interaction with virus antigens. A conclusion was made that the detection of anti-HTLV-111 in systemic rheumatoid diseases was a false-positive result.

Antibodies, Viral↗
PubMed1986

[Diagnostic preparations based on monoclonal antibodies].

The specific activity of diagnostic preparations based on monoclonal IgM MAK-14-7 interacting with the antigens of the Venezuela equine encephalomyelitis virus, monoclonal IgG KAMA-51 interacting with the antigens of the forest spring encephalitis virus and monoclonal IgA OKA and IgM OKA binding to the antigens of the vaccine virus was studied by the enzymatic immunological and immunofluorescent tests. Both the ascitic preparations of the antibodies and the fluorescein isothiocyanate-conjugated reagents such as MAK-FITC, KAMA-FITC and OKA-FITC were characterized by high specificity and activity. The specific sensitivity and activity of the monoclonal immunoglobulins was completely maintained in the mixed and combined preparations.

Animals↗
PubMed1986

[Optimization of conditions for preparation of the solid phase of infected cells in the immunoenzyme analysis of monoclonal antibodies].

The conditions of immunoenzyme assay have been studied on the solid state phase of infected cells using the model of monoclonal antibodies MAK-14-7 to the virus of Venezuelan equine encephalomyelitis (VVEE) and monoclonal antibodies OKA-1 to vaccine virus in the systems of VNK-21 cells or 4647 cells infected by VVEE, or HeLa cells infected by vaccine virus. The titer of monoclonal antibodies detected grows with the dose of infected cells fixed in the holes of micropanel used for reaction and with the multiplicity of infection. The most intensive and contrasting dyeing of conjugate has been registered when the cells have been fixed with 0.25% glutaraldehyde 24 h after infection. The titers of ascytic preparations of monoclonal antibodies MAK-14-7 and OKA-1 under the optimal conditions of immunoenzyme assay reaction on the solid phase of infected cells present 1 : 10 000 and 1 : 100 000.

Animals↗
PubMed1983

[Determination of the species specificity of interferons in the translation of the their mRNA from various cell cultures].

Interferons obtained on induction of human lymphocytes with Newcastle viruses and staphylococcal enterotoxin A and diploid fibroblast cells of human embryos with poly (I).poly (C), as well as translation products of interferon mRNA obtained from these cells were analysed serologically. It was shown that the main type of interferon produced by the cells depended on the cell culture and inductor nature. It was defined at the level of the respective gene depression. Effective translation of mRNA of the interferons of the 3 types makes possible production of cDNA and creation of bacterial plasmids coding the genetic information for the synthesis of human interferon.

Cells, Cultured↗
PubMed1983

[Purification and identification of mRNA of immune (gamma) interferon from human splenocytes].

The isolation of total RNA from primary culture of human splenocytes and its physico-chemical and biological properties are described. Human splenocytes are characterized by a high content of mRNA of human immune interferon, low content of total RNA and an extremely high activity of RNAases. Therefore it was necessary to elaborate conditions for the isolation of mRNA without DNA contaminants in the presence of extensive inhibitors of the RNAase activity. These include cell homogenization, separation of cytoplasm at -10 degrees C and treatment by RNAase inhibitors--ribonucleoside-vanadyl complexes or a combination of aurin-tricarboxylic acid with dithiothreitol. The resulting preparations of total RNA were purified by chromatography on oligo (dT)-cellulose and translated in a cell-free system from rabbit reticulocytes. These preparations were free of nonspecific translation inhibitors which are normally present in the lymphoid cells mRNA. In a cell-free system mRNA of human splenocytes induced with staphylococcal enterotoxin A code the synthesis of biologically active interferon which was identified as immune (gamma) human interferon, using a serological analysis. The preparations of immune interferon mRNA obtained under the conditions described above can further be used for cloning of the corresponding gene in bacterial cells.

Chromatography, Affinity↗
PubMed1982

[Effect of remantadine and ribavirin on the production and antiviral action of human interferon types I and II].

The results of using human interferon of types I (alpha- and beta-interferons) and II (gamma-interferon) in combination with chemotherapeutic drugs, such as remantadin and ribavirin for the study in human cell cultures are presented. Moderate doses of the drugs (25 microgram/ml) did not eliminate the interferon production. In higher doses (50 microgram/ml) they lowered the interferon production levels 2--3 times. In the presence of ribavirin the level of the interferon production lowering was higher. On the whole the effect of the drugs on production of interferons of types I and II was of a similar character despite the different means of interferon induction. The combined use of interferons of types I and II with the chemotherapeutic drugs in human embryo cultures infected with Semiliki forest virus (SFV) revealed an additive character of the antiviral effect in all combinations tested. The level of the antiviral activity of alpha-, beta- and gamma-interferons against the SFV was practically the same.

Adamantane↗
PubMedDate not supplied

[Activity of "Ferrovir" preparation towards RNA and DNA viruses].

Ferrovir (trivalent iron in complex with native sturgeon milt DNA) is nontoxic, its 50% inhibiting concentration (IC50) is at least 4000 micrograms/ml, 90% effective concentration (EC90) towards HIV-1 is 800 micrograms/ml. These effects do not depend on the cell culture or individual biological characteristics and subtypes of 7 strains of HIV-1 used in our study. The chemotherapeutic index of the drug is more than 20. Combined therapy with ferrovir and retrovir had an additive antiviral effect. Ferrovir reduced the titer of human CMV in fibroblast culture by 1-2 Ig TCD50. Ferrovir protected mice after intracerebral inoculation with lethal herpes simplex virus (type 1) (survival 33.7%, protection 27.1%, which is close to the reference group treated with zovirax). These facts evidence antiviral activity of ferrovir towards RNA and DNA viruses and prompt further study of this drug with the aim of its clinical application.

Animals↗
PubMedDate not supplied

[The characteristics of human immunodeficiency virus strains isolated from HIV-infected persons on the territory of the USSR].

Fifty-seven HIV-1 strains were isolated from peripheral blood lymphocytes of 102 HIV-infected persons involved in epidemic outbreaks in different cities of the USSR. The effectiveness of isolation was 29.1% in asymptomatic infection, 51.7% in cases with generalized lymphadenopathy, and 82.6% in persons with severe clinical manifestations. Identification of the isolates by indirect immunofluorescence, ELISA, reverse transcriptase activity, Western blot, electron microscopy, and polymerase chain reaction showed them to belong to HIV-1 type. Reproduction of the isolates in cell cultures was accompanied by cytopathic effect and syncytium formation. The isolated strains can be divided into two groups: (1) the poorly growing isolates with low or negative RT and ELISA results and (2) the isolates with high infectivity, broad spectrum of cell tropism, and high levels of RT and ELISA. These data show the correlation of biological properties of HIV-1 strains isolated in the USSR with those of HIV strains previously isolated in Europe, USA, and Africa.

Cell Line↗