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D Nachmansohn

Publications and source records attributed to D Nachmansohn.

At least 19 recordsLinked to original sources
PubMed1977

Nerve excitability: transition from descriptive phenomenology to chemical analysis of mechanisms.

The electrical activity by which impulses are conducted along nerve and muscle fibers, is carried by Na-and K-ions moving across the excitable membranes due to increased ion permeability. -- A biochemical approach, initiated to elucidate the mechanism of the permeability changes, centered around the analysis of the properties and functions of the proteins, including enzymes, directly associated with the role of AcCh, in the excitable membrane. The results necessitated a fundamentally reformed concept of the role of AcCh. The four proteins specifically associated with the function of AcCh form a cycle which controls the rapid ion permeability changes of the membrane and permits the ion fluxes through dynamic gateways. A model has been elaborated that integrates biochemical, biophysical, and thermodynamic data; it permits the interpretation of many electrophysiological data in molecular terms. AcCh has basically the same function in conducting and synaptic parts of excitable membranes. The new concept has replaced the purely descriptive phenomenology of nerve impulse propagation by the analysis of the chemical mechanisms of nerve excitability and bioelectricity.

Acetylcholine↗
PubMed1976

Transduction of chemical into electrical energy.

The paper recalls some fundamental notions, developed by Otto Meyerhof, which were used in the analysis of the transduction of chemical into mechanical energy during muscular contraction. These notions formed the basis of the approach to the analysis of the transduction of chemical into electrical energy, i.e., the very principle underlying nerve and muscle excitability and bioelectricity. Instrumental for this purpose was the use, since 1937, of electric organs of fish, a tissue highly specialized for bioelectrogenesis.

Acetylcholine↗
PubMed1975

Nerve excitability--toward an integrating concept.

Although numerous experimental data have been accumulated in the various fields of research on bioelectricity, the mechanism of nerve excitability is still an unsolved problem. Many mechanistic interpretations of nerve behavior cover only a part of the facts, are thus selective and unsatisfactory. An attempt at an integral interpretation of basic data well-established by electrophysiological, biochemical, and biophysical investigations was inspired by the late Aharon Katchalsky and a first attempt had been made previously (Neumann et al., 1973). The present account is a further step toward a quantitative physiochemical theory of bioelectricity. We have further explored the previously introduced notion of a basic excitation unit in excitable membranes. This notion is of fundamental importance for modeling details of sub- and suprathreshold responses, such as threshold behavior and strength-duration curves, in terms of kinetic parameters for specific membrane processes. Our integral model of excitability is based on the original chemical hypothesis for the control of bioelectricity (Nachmansohn, 1959, 1971b). This specific approach includes some frequently ignored experimental facts on acetylcholine-processing proteins in excitable membranes. According to the integral model, acetylcholine ions are continuously processed through the basic excitation units within excitable membranes: axonal, presynaptic, and postsynaptic parts. Excitability, i.e., the generation and propagation of nerve impulses, is due to a cooperative increase in the rate of AcCh translocation through the cholinergic control system.

Acetylcholine↗
PubMed1973

An attempt at an integral interpretation of nerve excitability.

A qualitatively consistent integral interpretation of biochemical, electrophysiological, and biophysical data on nerve activity is given in terms of a basic excitation unit. This operational term models a dynamically coupled assembly of membrane components accounting for graded and all-or-none responses upon stimulation. The analysis contains a series of suggestions linking controversial interpretations and is aimed at stimulation of experimental studies providing the basis for a quantitative integral theory of nerve excitation.

Acetylcholine↗
PubMed1971

Chemical events in conducting and synaptic membranes during electrical activity.

Evidence has accumulated in recent years for the central role of proteins and enzymes in the function of cell membranes. In the chemical theory proposed for the generation of bioelectricity, i.e., for the control of the ion permeability changes of excitable membranes, the protein assembly associated with the action of acetylcholine plays an essential role. Support of the theory by recent protein studies in which the excitable membranes of the highly specialized electric tissue were used will be discussed. A scheme is presented indicating the possible sequence of chemical reactions that change ion permeability after excitation. A sequence of chemical events within the excitable membranes of the synaptic junctions, i.e., within the pre- and postsynaptic membranes, similar to that proposed for the conducting membranes, is presented in a second scheme as an alternative to the hypothesis of the role of acetylcholine as a transmitter between two cells.

Acetylcholine↗
PubMed1969

Photoregulation of biological activity by photochromic reagents. 3. Photoregulation of bioelectricity by acetylcholine receptor inhibitors.

The photochromic compounds N-p-phenylazophenyl-N-phenylcarbamylcholine chloride and p-phenylazophenyltrimethylammonium chloride inhibit the carbamylcholine-produced depolarization of the excitable membrane of the monocellular electroplax preparation of Electrophorus. The trans isomer of each predominates in the light of a photoflood (420 mmu) lamp; they are stronger inhibitors than the cis isomers, which predominate under ultraviolet (320 mmu) irradiation. The potential difference across the excitable membrane may be photoregulated by exposing an electroplax in the presence of a solution of carbamylcholine and either of the two compounds to light of appropriate wavelengths, since light shifts the cis-trans equilibrium. The system may be considered as a model illustrating how one may link a cis-trans isomerization, the first step in the initiation of a visual impulse, with substantial changes (20-30 mv) in the potential difference across an excitable membrane.

Acetylcholine↗