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Biomedical subjects

D Neumeier

Publications and source records attributed to D Neumeier.

70 records · Page 4Linked to original sources
PubMed1977

Radioimmunoassay for subunit B in isoenzymes CK-MB and CK-BB of creatine phosphokinase.

We report the development of a double-antibody system for radioimmunoassay of CK-B subunit in isoenzymes CK-MB and CK-BB of creatine phosphokinase. With our method, 4.1 ng/ml of isoenzyme CK-BB can be detected. Within-run and between-run coefficients of variation are respectively 5.3% and 19.6% Analytical recovery of added antigen is 98.9 +/- 14.7%. The CK-B concentration in sera from 20 healthy adults was less than 75 ng/ml. In sera from 25 patients with a rise in CK-total activity, but without CK-MB or CK-BB activity tested with the previous reported immunological methods, we found CK-B concentrations in the range 0--86 ng/ml. In contrast, in 23 sera from patients with acute myocardial infarction within the last 48 hours CK-B concentration was in the range 104--225 ng/ml.

Antibody Specificity↗
PubMed1977

Quantitative determination of creatine kinase isoenzyme catalytic concentrations in serum using immunological methods.

For the determination of creatine kinase isoenzyme catalytic concentrations in serum two methods based on immunological reactions are presented: One method uses inhibiting antibodies, which selectively block the activity of creatine kinase M subunits ("Inhibition Test"). This test is used for routine measurements of creatine kinase MB catalytic concentration; Another method uses precipitating antibodies, which allows a quantitative differentiation of creatine kinase isoenzymes MM, MB and BB ("Precipitation Test"). This test is used as a control for the Inhibition Test for the possible presence of creatine kinase BB activities in doubtful cases. Procedures, specificity, correlation and application of these methods are discussed.

Creatine Kinase↗
PubMed1977

[The use of primate tissue homogenates for quality control in the creatine kinase-MB determination with inhibiting antibodies (author's transl)].

The creatine kinase isoenzymes CK-MM, CK-MB and CK-BB from Rhesus monkey heart and brain tissue homogenates were separated chromatographically. Thereafter it could be demonstrated that the activity of the simian subunit CK-M is completely inhibited by anti-inhibitory-CK-M serum. Thus control sera from simian tissue are in principle suited for quality control in an immunological determination of creatine kinase-MB. The intra-assay variance and interassay variance were n = 56, -/x = 29.2 U/1, SD = 3.2 U/1, CV = 11.1% and n= 12, -/x = 166.7 U/1, SD = 5.0 U/1, CV = 3.0% respectively. It is desirable to develop control sera with catalytic concentrations of creatine kinase-MB in a lower range.

Animals↗
PubMed1976

[Determination of creatine kinase-MB in serum using inhibiting antibodies (author's transl)].

A new method for the determination of creatine kinase-MB activity in the serum is presented. The principle of this method is the direct measurement of the activity of creatine kinase M subunits by inhibiting antibodies. The total test procedure takes 15 min. In the sera of all the 83 patients tested, who have clinically proven myocard infarction, creatine kinase-MB activity can be measured between the 6th and 28th hour after infarction. At the time of maximum total creatine kinase activity the percentage of creatine kinase-MB activity is between 6 and 17%, the mean value being 8%. In cases of emergency this method can be used for the differential diagnosis of elevated total creatine kinase activities of unknown origin.

Clinical Enzyme Tests↗
PubMed1976

[Multiple myeloma with monoclonal IgE gammopathy (author's transl)].

In a 48-year-old female patient with monoclonal gammopathy and histologically proven plasmocytoma IgE could be demonstrated in bone marrow plasma cells by means of direct immunofluorescence. Immunoelectrophoresis showed a light-chain type chi. Radiographically diffuse osteolytic skeletal lesions were found. Bence-Jones proteinuria and plasma cell leukaemia were absent. This patient represents the fourth recognized case of IgE myeloma. The chi/lambda ratio in IgE myeloma is 1:1 according to present knowledge.

Bone Marrow↗
PubMed1976

Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction.

This is a new method for the determination of creatine kinase isoenzyme MB activity in serum. The method uses direct activity measurement of creatine kinase B subunit activity after blocking of CK-M subunit activity by inhibiting antibodies. The test takes no longer than 15 min. The method yields an intra-serial C.V. of 2.0-12.9%, and a C.V. from day to day of 5.5%. The detection limit is 3.4 U/l creatine kinase MB. In the 95 cases with proven myocardial infarction several types of creatine kinase MB activity kinetics could be determined. The percentage of creatine kinase MB of peak CK-total is 6-25%, with a mean of 11.1%. The amount of creatine kinase MB with respect to total CK activity after reinfarction is higher than the amount after initial infarction.

Antigen-Antibody Reactions↗
PubMed1975

[Determination of creatine phosphokinase-MB in the serum of patients with myocardial infarction by an immunological method (author's transl)].

The immunological method of determining creatine phosphokinase-MB in the serum of patients with myocardial infarction described here is based on the differential measurements of CK-activities before and after a specific immuno-precipitation of the CK-MB type. The minimum activity of the CK-MB-type which it is possible to determine with this method is 4% of the total activity. In patients with clinically confirmed myocardial infarctions 1-15% (mean 7.8%) of the total activity can be calculated as CK-activity of the MB-type on the first/second day(s) after the infarction. In patients with increased total CK-activity and without verified infarction the CK-MB content does not differ significantly from zero. The differences between the two groups are statistically significant. In patients with myocardial reinfarction the CK-MB-activity is higher than that after the first infarction. The immunological method to determine creatine-phosphokinase isoenzyme MB is of differential-diagnostic value in myocardial infarction.

Clinical Enzyme Tests↗