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Biomedical subjects

D Ng

Publications and source records attributed to D Ng.

At least 37 records · Page 2Linked to original sources
PubMed1998

Enhancement of allergic inflammation by the interaction between diesel exhaust particles and the immune system.

There is growing evidence that fossil fuel combustion products act as adjuvants in the immune system and may lead to enhancement of allergic inflammation. Through this mechanism, particulate air pollutants may be an important contributor to the increased prevalence and morbidity of asthma and allergic rhinitis. In this communication we focus on the role of diesel exhaust particles (DEPs) in skewing the immune response towards IgE production and induction of allergic inflammation. We review experimental studies in animals and humans showing that DEPs enhance IgE production by a variety of mechanisms, including effects on cytokine and chemokine production, as well as activation of macrophages and other mucosal cell types. We discuss metabolic and cellular activation pathways linked to chemicals such as polycyclic aromatic hydrocarbons contained in DEPs and demonstrate how these molecular events may impact cytokine, chemokine, and accessory molecule expression in the immune system.

Animals↗
PubMed1997

Cone-rod dystrophy due to mutations in a novel photoreceptor-specific homeobox gene (CRX) essential for maintenance of the photoreceptor.

Genes associated with inherited retinal degeneration have been found to encode proteins required for phototransduction, metabolism, or structural support of photoreceptors. Here we show that mutations in a novel photoreceptor-specific homeodomain transcription factor gene (CRX) cause an autosomal dominant form of cone-rod dystrophy (adCRD) at the CORD2 locus on chromosome 19q13. In affected members of a CORD2-linked family, the highly conserved glutamic acid at the first position of the recognition helix is replaced by alanine (E80A). In another CRD family, a 1 bp deletion (E168 [delta1 bp]) within a novel sequence, the WSP motif, predicts truncation of the C-terminal 132 residues of CRX. Mutations in the CRX gene cause adCRD either by haploinsufficiency or by a dominant negative effect and demonstrate that CRX is essential for the maintenance of mammalian photoreceptors.

Adult↗
PubMed1995

Evaluation of an intelligent seat system.

This study was conducted to evaluate the effect of an intelligent seat system, a microprocessor-based interactive seat that automatically adjusts itself to fit a seated individual by making pressure-sensitive adjustments on its own. First, a standard American automobile seat ('baseline' seat) was assessed for comfort. Subjective ratings of comfort, pressure distribution and seated anthropometric measurements were recorded for 20 test subjects. These measurements were recorded while the subjects maintained a simulated driving position in a seat buck. The comfort scale was based on a rating of 1 to 10, with 1 corresponding to 'very poor' and 10 corresponding to 'very good.' Based on a nonlinear, multiple regression model that had been previously developed, the comfort rating of the seat was predicted based on the subjective ratings and the recorded values of 450 pressure measurements from 20 subjects. The predicted comfort value was 7.46 for the baseline seat. Following the baseline assessment, the intelligent seat system was installed into the standard American automobile seat. The objective and subjective assessments were then repeated for 17 subjects and the new predicted comfort rating was 8.06. A t-test performed on the subjective and objective measures indicated that this was a significant improvement in seat comfort. Overall, subjects felt the self-adjusting seat was more customized and more comfortable, providing a better fit.

Journal Article↗
PubMed1995

The prevalence of Helicobacter pylori infection in different countries.

The prevalence of Helicobacter pylori infection in a community is related to three factors: firstly, the rate of acquisition of infection with H. pylori--that is, incidence; secondly, the rate of loss of the infection; thirdly, the prolonged persistence of the bacterium in the gastroduodenal mucosa between infection and eradication. Variation in the prevalence of H. pylori is dominated by the great differences between communities in the incidence of H. pylori infection during childhood. The countries of the world form two groups: Group One is made up of those where the majority of children become infected with H. pylori during childhood and chronic infection continues during adult life; in Group Two only a minority of children are infected during childhood, but the prevalence of infection rises in proportion to age during adult life. Understanding the ages at which people acquire infection with H. pylori is crucial to the interpretation of H. pylori prevalence data.

Adult↗
PubMed1994

Protein tyrosine phosphorylation in Mycobacterium tuberculosis.

Crude cell extracts from three strains of Mycobacterium tuberculosis were analyzed for the presence of proteins possessing phosphorylated tyrosine residues. A protein migrating at approximately 55 kDa was detected using an antiphosphotyrosine monoclonal antibody. In addition, less predominant bands were observed between 50 kDa and 60 kDa. That M. tuberculosis contains specific tyrosine phosphorylated proteins implies that M. tuberculosis has tyrosine kinase activity. Examination of other, non-pathogenic mycobacterium species yielded no major antiphosphotyrosine reactive proteins. This suggests that the antiphosphotyrosine reactive protein is specific to M. tuberculosis strains. These results provide evidence that M. tuberculosis contains an antiphosphotyrosine reactive protein.

Antibodies, Monoclonal↗
PubMed1994

Initial evaluation of human recombinant interleukin-1 receptor antagonist in the treatment of sepsis syndrome: a randomized, open-label, placebo-controlled multicenter trial.

OBJECTIVES: To evaluate the safety, pharmacokinetics, and efficacy of human recombinant interleukin-1 receptor antagonist (IL-1ra) in the treatment of patients with sepsis syndrome. DESIGN: Prospective, open-label, placebo-controlled, phase II, multicenter clinical trial using three different doses of human recombinant IL-1ra. SETTING: Twelve academic medical center intensive care units in the United States. PATIENTS: Ninety-nine patients with sepsis syndrome or septic shock who received standard supportive care and antimicrobial therapy, in addition to infusion with escalating doses of IL-1ra or placebo. INTERVENTIONS: Patients received an intravenous loading dose of either human recombinant IL-1ra (100 mg) or placebo, followed by a 72-hr intravenous infusion of either one of three doses of IL-1ra (17, 67, or 133 mg/hr) or placebo. All patients were evaluated for 28-day, all-cause mortality. MEASUREMENTS AND MAIN RESULTS: A dose-dependent, 28-day survival benefit was associated with IL-1ra treatment (p = .015), as indicated by the following mortality rates: 11 (44%) deaths among 25 placebo patients; eight (32%) deaths among 25 patients receiving IL-1ra 17 mg/hr; six (25%) deaths among 24 patients receiving IL-1ra 67 mg/hr; and four (16%) deaths among 25 patients receiving IL-1ra 133 mg/hr. A dose-related survival benefit was observed with infusion of IL-1ra in patients with septic shock at study entry (n = 65; p = .002) and in patients with Gram-negative infection (n = 45; p = .04). Patients with an increased circulating interleukin-6 (IL-6) concentration of > 100 pg/mL at study entry demonstrated a dose-related survival benefit with IL-1ra treatment (p = .009). In patients with an increased IL-6 concentration at study entry, the magnitude of the decrease in IL-6 concentration 24 hrs after the initiation of therapy was correlated with increasing the IL-1ra treatment dose (p = .052). A significant dose-related reduction in the Acute Physiology and Chronic Health Evaluation (APACHE II) score was achieved by the end of infusion (p = .038). A renal elimination mechanism for IL-1ra was suggested by the positive correlation between IL-1ra plasma clearance and estimated creatinine clearance (p = .001; r2 = .51). Human recombinant IL-1ra was well tolerated. CONCLUSIONS: This initial evaluation suggests that human recombinant IL-1ra is safe and may provide a dose-related survival advantage to patients with sepsis syndrome. A larger, definitive clinical trial is needed to confirm these findings.

Adult↗
PubMed1994

Twenty-five years of national service--changes in height, weight and body mass index.

The height and weight of 18-year-old Singapore National Service enlistees were extracted from computer records spanning 25 years (1967-1991). These were analysed for mean height, weight and body mass index (BMI) which overall show increases of 4.12 cm, 10.13 kg and 2.60 kg/m2 respectively. All ethnic groups (Chinese, Malay, Indian and Others) show a similar rising trend. While mean height appears to have stabilised in recent years, mean weight continues to rise. This is reflected in an increasing mean BMI. The proportion of 18-year-old male recruits with a BMI in the overweight range (> or = 25 kg/m2) increased from 1.37% in 1967 to 12.07% in 1991.

Adolescent↗
PubMed1993

Oral pilocarpine for post-irradiation xerostomia in patients with head and neck cancer.

BACKGROUND AND METHODS: We evaluated pilocarpine hydrochloride for the treatment of radiation-induced xerostomia, a common complication of irradiation of the head and neck. A prospective, randomized, double-blind, placebo-controlled trial was undertaken to test the safety and efficacy of pilocarpine, particularly in reversing the decrease in the production of saliva and other manifestations of xerostomia. Patients received either placebo or pilocarpine (5 mg or 10 mg orally three times a day) for 12 weeks and were evaluated at base line and every 4 weeks. RESULTS: We studied 207 patients who had each received > or = 4000 cGy of radiation to the head and neck. In the patients receiving the 5-mg dose of pilocarpine, oral dryness improved in 44 percent, as compared with 25 percent of the patients receiving placebo (P = 0.027). There was overall improvement in 54 percent of the 5-mg group as compared with 25 percent of the placebo group (P = 0.003), and 31 percent of the 5-mg group had improved comfort of the mouth and tongue, as compared with 10 percent of the placebo group (P = 0.002). Speaking ability improved in 33 percent of the 5-mg group as compared with 18 percent of the placebo group (P = 0.037). Saliva production was improved, but it did not correlate with symptomatic relief. There were comparable improvements in the group receiving the 10-mg dose. The primary adverse effect was sweating, in addition to other minor cholinergic effects. Six and 29 percent of the patients in the 5-mg and 10-mg groups, respectively, withdrew from the study because of adverse effects. There were no serious adverse effects related to pilocarpine. CONCLUSIONS: Pilocarpine improved saliva production and relieved symptoms of xerostomia after irradiation for cancer of the head and neck, with minor side effects that were predominantly limited to sweating.

Administration, Oral↗
PubMed1993

Fine mapping of the three rRNA operons on the updated genomic map of Campylobacter jejuni TGH9011 (ATCC 43431).

The three rRNA gene loci of Campylobacter jejuni TGH9011 (ATCC 43431) were cloned. All three rRNA operons were shown to possess a contiguous 16S-23S structure and contain intercistronic tRNA(Ala) and tRNA(Ile). The three RNA operons and additional 14 genetic markers were mapped in the updated genomic map of C. jejuni TGH9011, which now has a total of 24 genetic markers.

Campylobacter jejuni↗