Biomedical subjects

D Novopashina

Publications and source records attributed to D Novopashina.

3 recordsLinked to original sources

PubMed2004

Binary hammerhead ribozymes with high cleavage activity.

Binary hammerhead ribozymes consisted of two oligoribonucleotides capable of assembling into hammerhead structure (without loop II) on the RNA target were engineered. Catalytic activities of such ribozymes were investigated in comparison with their full-length analog and ribozyme where two strands were jointed by non-nucleotidic linker. Binary constructs were shown to be significantly more active than the parent full-length hammerhead ribozyme.

Catalysis↗

PubMedDate not supplied

2'-O-modified oligoribonucleotides with terminal 3'-3'-internucleotide linkage and their derivatives.

A high RNA binding affinity and nuclease resistance of 2'-O-modified (2'-O-methyl, 2'-O-tetrahydropyranyl) oligoribonucleotides containing the "inverted" T at the 3'-end have been shown. The synthesis and properties of new photoactivatable perfluoroarylazide derivatives of these oligoribonucleotides are discussed.

Kinetics↗

PubMedDate not supplied

Conjugates of oligo(2'-O-methylribonucleotides) with minor groove binders as new sequence-specific agents recognizing both grooves of double-stranded DNA.

Design, synthesis and physico-chemical studies of new pyrimidine oligo(2'-O-methylribonucleotide) conjugates with one or two oligo(pyrrolecarboxamide) minor groove binders (MGB) are described.

Base Sequence↗